Cooking characteristics,chemical composition and protein quality of newly developed genotypes of pigeonpea (Cajanus cajan L)

Eight newly developed pigeonpea genotypes (ICPL 87, ICPL 151, ICPL 270, ICPL 366, ICPL 87051, ICPL 87063, ICPL 87067, and ICPV 1), and the two controls (BDN 2 and C 11) were analysed for cooking quality parameters and chemical composition, including amino acids and minerals. Protein quality was evaluated by determining the true protein digestibility, biological value, net protein utilisation (NPU), and utilisable protein. These genotypes differed significantly (P < 0–01) in the dhal cooking time. Sensory properties of dhal of these genotypes were found to be within the acceptable range, even though there were considerable differences among genotypes. Dhal protein, calcium, magnesium, zinc, and iron contents of these genotypes showed noticeable differences. Calcium content of ICPL 87067 was the highest (85-6 mg per 100 g) and of ICPL 87 the lowest (54-4 mg per 100 g) indicating large differences among the newly developed genotypes. No noticeable differences in sulphur-containing amino acids of these genotypes were observed. NPU was the highest (65–4%) for ICPL 366 and the lowest (56–6%) for ICPL 270 and ICPL 87067 indicating significant (P < 0–01) differences among genotypes studied.     

木豆小曲白酒发酵工艺的研究

采用小曲固态发酵工艺,通过正交实验和单因子水平实验,确定了以木豆为主要原料,高粱为辅助原料,酿造小曲白酒的最佳发酵工艺。实验结果表明,木豆小曲白酒的最佳发酵工艺为:采用直接入罐发酵的方法,木豆与高粱配比为1∶1,酒曲用量为1.2%,糖化酶用量为0.4%,高活性酵母用量为0.5%,发酵时间为30d,出酒率以50%(v/v)计可达到54.8%。     

Field infestation of pigeonpea (Cajanus cajan (L.) Millsp.) by Callosobruchus chinensis (L.) in Uganda

Studies were conducted in Uganda to determine the importance of field infestation of pigeonpea by bruchids, and whether or not the susceptibility of the pigeonpea pod to infestation changes during pod and seed development. A survey of farmers’ fields in the major pigeonpea growing areas in northern Uganda showed that bruchids did infest pigeonpea in the field. In all three districts surveyed (Apac, Gulu and Lira), infestation levels were similar and by one species only, Callosobruchus chinensis. Once harvested, infestation of stored seeds was four times greater than stored pods. In a separate study, caged pigeonpea pods were infested at seven different developmental stages by C. chinensis. The number of eggs laid increased with development, from pod formation to the mature yellow stage, but declined thereafter. When pods were incubated, there was no adult emergence from pods infested after they had matured and started to dry. The frequency of C. chinensis adult emergence varied significantly over time; a bimodal frequency pattern of emergence was observed from pods infested at mature green stage and, to a lesser extent at the preceding late pod filling state, suggesting the occurrence of polymorphism.     

Effects of nitrogen supply on cassava/pigeonpea intercropping with three contrasting cassava cultivars

Three cassava cultivars of contrasting canopy size were grown as a sole crop and in intercropping with pigeonpea under two N supply levels to examine whether the effect of N fertilizer was different in sole crop cassava and in intercropping, and whether it depended on the canopy type of cassava cultivar. Application of N promoted plant height and canopy width of cassava in both cropping systems, and this resulted in increased competitiveness of cassava in intercropping. However, when the most vigorous cassava cultivar was used in intercropping, N supply caused excessive top growth with no increase in tuber yield. The yield of pigeonpea on the other hand was always reduced with N supply in intercropping with any cassava cultivar.     

香酥木豆的加工工艺研究

研究了香酥木豆的加工工艺.对浸泡工序、分选工序及油炸工序进行详细探讨,结果确定了浸泡工艺参数:先用0.1%～2.0% NaOH溶液浸泡8～16h,再用3%～15%明矾溶液浸泡8～16h;分选工艺参数:0.5～0.7cm孔径的网筛,沥水1～3h;油炸工艺参数:油温190～220℃,油炸时间8～11min,所制成品达到国家标准GB16565-1996.     

木豆叶球松素的超声提取工艺及其抗氧化活性研究

在考察单因素(甲醇浓度、提取时间、提取温度和料液比)实验条件对木豆叶中球松素提取率影响的基础上,采用正交实验设计法优化得到球松素的最佳提取工艺条件,同时通过DPPH自由基清除实验评价了提取物的抗氧化活性.结果表明,木豆叶中球松素超声提取的最佳工艺条件为,甲醇提取浓度80％,提取时间60min,提取温度50℃,料液比1∶40(g/mL),球松素的提取率可达到1.832mg/g;甲醇提取物清除自由基的IC50值为0.281 mg/mL.本研究建立的木豆叶中球松素的超声提取工艺具有提取效率高、操作简便等优点,适合木豆叶中球松素的提取,且甲醇提取物具有良好的抗氧化活性.     

Comparative TMT Proteomic Analysis Unveils Unique Insights into Helicoverpa armigera (Hübner) Resistance in Cajanus scarabaeoides (L.) Thouars

Pigeonpea [Cajanus cajan (L.) Millspaugh] is an economically important legume playing a crucial role in the semi-arid tropics. Pigeonpea is susceptible to Helicoverpa armigera (Hübner), which causes devastating yield losses. This pest is developing resistance to many commercially available insecticides. Therefore, crop wild relatives of pigeonpea, are being considered as potential sources of genes to expand the genetic base of cultivated pigeonpea to improve traits such as host plant resistance to pests and pathogens. Quantitative proteomic analysis was conducted using the tandem mass tag platform to identify differentially abundant proteins between IBS 3471 and ICPL 87 tolerant accession and susceptible variety to H. armigera, respectively. Leaf proteome were analysed at the vegetative and flowering/podding growth stages. H. armigera tolerance in IBS 3471 appeared to be related to enhanced defence responses, such as changes in secondary metabolite precursors, antioxidants, and the phenylpropanoid pathway. The development of larvae fed on an artificial diet with IBS 3471 lyophilised leaves showed similar inhibition with those fed on an artificial diet with quercetin concentrations with 32 mg/25 g of artificial diet. DAB staining (3,3′-diaminobenzidine) revealed a rapid accumulation of reactive oxygen species in IBS 3471. We conclude that IBS 3471 is an ideal candidate for improving the genetic base of cultivated pigeonpea, including traits for host plant resistance.     

木豆豆沙的加工工艺研究

对木豆豆沙不同的加工方法进行了初步研究,确定了豆沙的最佳加工工艺,研制出具特殊风味、营养丰富的木豆豆沙。