螺杆泵专用抽油杆试验研究

以往大庆油田螺杆泵井所用抽油杆柱大多都是沿用抽油机井上使用的普通连接结构抽油杆,这种抽油杆适合于上下往复运动,抗拉强度是抽油杆的主要抗力指标。而螺杆泵井所用抽油杆,其主要运动形式为旋转运动,承力方式也由抗拉变成抗扭,使得普通连接结构抽油杆应用在螺杆泵井上以后,经常出现断脱事故。针对这些现象,研制了插接式螺杆泵专用抽油杆和锥螺纹式螺杆泵专用抽油杆。文章介绍了2种结构抽油杆的防断脱原理、优化设计方法、室内检测及在大庆油田的使用情况,并针对现场出现的典型事故进行了分析,提出了相应的改进意见。     

AUTOCONER-338型自动络筒机的接头工艺与切疵有效性分析

介绍了AUTOCONER-338型自动络筒机空气捻接器的接头要求与影响接头质量的因素,并且就不同品种的纱线选用不同接头工艺进行了简单分析;介绍了如何评价络筒切疵有效性与影响切疵率的因素.通过以上2方面的分析介绍,及时调整设计与工艺,提高了生产效率,改善了布面质量.     

带肋钢筋套筒挤压连接的质量控制

针对太原可口可乐综合厂房主筋采用φ28、φ32三级钢存在的可焊性差的问题，提出了在施工中采用套筒挤压连接的方式，并对该方法的操作要点及质量控制措施进行了总结。     

Tau Exon 10 Inclusion by PrPC through Downregulating GSK3β Activity

Tau protein is largely responsible for tauopathies, including Alzheimer’s disease (AD), where it accumulates in the brain as insoluble aggregates. Tau mRNA is regulated by alternative splicing, and inclusion or exclusion of exon 10 gives rise to the 3R and 4R isoforms respectively, whose balance is physiologically regulated. In this sense, one of the several factors that regulate alternative splicing of tau is GSK3β, whose activity is inhibited by the cellular prion protein (PrP^C), which has different physiological functions in neuroprotection and neuronal differentiation. Moreover, a relationship between PrP^C and tau expression levels has been reported during AD evolution. For this reason, in this study we aimed to analyze the role of PrP^C and the implication of GSK3β in the regulation of tau exon 10 alternative splicing. We used AD human samples and mouse models of PrP^C ablation and tau overexpression. In addition, we used primary neuronal cultures to develop functional studies. Our results revealed a paralleled association between PrP^C expression and tau 4R isoforms in all models analyzed. In this sense, reduction or ablation of PrP^C levels induces an increase in tau 3R/4R balance. More relevantly, our data points to GSK3β activity downstream from PrP^C in this phenomenon. Our results indicate that PrP^C plays a role in tau exon 10 inclusion through the inhibitory capacity of GSK3β.     

Antisense Oligonucleotide-Based Rescue of Aberrant Splicing Defects Caused by 15 Pathogenic Variants in ABCA4

The discovery of novel intronic variants in the ABCA4 locus has contributed significantly to solving the missing heritability in Stargardt disease (STGD1). The increasing number of variants affecting pre-mRNA splicing makes ABCA4 a suitable candidate for antisense oligonucleotide (AON)-based splicing modulation therapies. In this study, AON-based splicing modulation was assessed for 15 recently described intronic variants (three near-exon and 12 deep-intronic variants). In total, 26 AONs were designed and tested in vitro using a midigene-based splice system. Overall, partial or complete splicing correction was observed for two variants causing exon elongation and all variants causing pseudoexon inclusion. Together, our results confirm the high potential of AONs for the development of future RNA therapies to correct splicing defects causing STGD1.     

15～60°钢丝帘布裁断机的性能及特点

介绍了15~60°钢丝帘布裁断机的几个主要机构,导开、输送、拼接、纵向切割、包边等的特点,列举了整机的主要技术参数,以说明该设备的先进性。     

钢筋套筒灌浆连接技术有限元分析

对于预制装配混凝土结构关键技术之一的钢筋套筒灌浆连接技术,已进行了不少试验研究,但试验不确定因素较多,离散性大.为研究这种连接在各种作用下的受力性能,拓展模型试验研究的成果,有必要建立可靠正确的有限元模型.本文在已有试验的基础上,采用ABAQUS软件,模拟钢筋套筒灌浆连接的受力性能.数值模拟结果与试验吻合良好,说明所建立的有限元模型正确,可以用于进一步参数分析和机理研究.     

基于超像素和光流引导的正射影像拼接方法

影像拼接是生成大规模数字正射影像的关键技术之一,但现有的影像拼接方法在进行多个影像拼接时存在拼接线穿过明显地物导致的鬼影现象。光流是观察者和场景间相对运动引起的影像边缘等的相对运动,其中,大光流对应影像间的变化区域,可用于检测正射影像间的明显地面区域。提出一种基于光流引导的新型影像拼接方法,通过超像素的密集光流提取影像中明显的地物信息,以避免接缝穿过明显的地面物体。采用由粗到细的接缝线优化策略,并在超像素级别上利用Dijkstra算法进行最佳拼接区域检测,从而提高接缝线检测的效率。在此基础上,结合归一化互相关成本函数在像素级别上进行拼接线的像素级优化,获得最优的接缝线。实验结果表明,该方法从主观视觉上能够生成高质量的接缝线,在保证拼接效率的情况下,SSIM质量评价指标较Dijkstra方法、图割方法以及商业软件OrthoVista得到明显提高。     

一种改进的Affymetrix外显子芯片原始数据分析方法

选择性剪切与许多人类疾病有关,基因以及基因异构体水平的表达分析是揭示选择性剪切变化情况的常用研究方法,Affymetrix外显子芯片为测量基因以及基因异构体表达水平提供了一种重要方法.由于外显子芯片基于杂交技术进行设计,实验数据中存在大量噪声,并且选择性剪切导致一个探针往往对应多个剪切异构体,这些给剪切异构体表达水平的计算带来了挑战.为此在先前提出的基于伽玛分布的概率模型(Gamma model for exon array data,GME)基础上,提出了iGME模型,进行基因以及异构体表达水平的计算.该模型利用已知的基因剪切异构体与探针的对应关系,模拟了条件独立的探针特性.通过采用真实实验数据进行验证,并与传统方法进行比较,结果表明iGME模型获得了较高的计算精度和更快的计算速度.     

光子晶体与单模光纤熔接对超连续谱展宽的影响

针对光子晶体光纤之间直接熔接损耗较大的问题.文中采用纳秒激光器作为泵浦源,通过光子晶体光纤与单模光纤HI-1060低损耗熔接的方法,研究了超连续谱的展宽过程,分析了超连续谱的产生机理.实验结果表明：泵浦源在重复频率为150kHz、泵浦功率为2.2W时,利用20m的光子晶体光纤与1m的单模光纤的熔接实现了输出功率为0.48W、光谱范围超过1100nm的超连续谱输出.