Effect of solar UV‐B exclusion on the phytoplankton community in a sub‐tropical mountain reservoir: a mesocosm study

A mesocosm experiment was conducted over a 4 week period to investigate the response of a phytoplankton community in a mountain freshwater reservoir to solar UV‐B exclusion. The mesocosms were filled with water taken from the depth of 0.30 m in the reservoir, and exposed to near‐ambient solar UV‐B (+UV‐B) and solar UV‐B exclusion (?UV‐B). The resulting chlorophyll‐a (Chl‐a), carotenoids, soluble sugars, algal composition and algal abundance were analysed. The study results indicated that carotenoids, carbohydrates and species distribution were affected under the ?UV‐B treatment. The carotenoid concentration was generally higher under the +UV‐B treatment than under the ?UV‐B treatment. The sucrose and glucose concentrations were affected differently by the UV‐B treatments, although both sugars strongly decreased under the +UV‐B treatment by the end of the experiment. The sucrose and glucose concentrations exhibited similar distribution patterns to those of carotenoids for the +UV‐B treatment, suggesting that these sugars could be directly involved in secondary metabolism. In contrast, the fructose content did not exhibit significant differences between UV‐B treatments. The Chl‐a concentration exhibited a progressive decrease during the experiment for both UV‐B treatments. Although no significant impact of UV‐B exclusion on the Chl‐a concentration was observed in this study, a slightly higher concentration was observed for the ?UV‐B treatment. The total phytoplankton biovolume (BV) exhibited a pattern similar to that of the Chl‐a concentration. Analysis of the phytoplankton composition revealed 52 species. However, because some species and classes were extremely difficult to identify, only 46 species from seven classes (Cyanophyceae, Chlorophyceae, Bacillariophyceae, Euglenophyceae, Charophyceae, Dinophyceae, Xanthophyceae) were unambiguously identified and considered for the UV‐B treatments. Chlorophyceae, Bacillariophyceae and Cyanophyceae comprised about 78% of the identified species. Aulacoseira granulata and Cyclotella meneghiniana, followed by Ceratium hirundinella, were the most abundant species. Principal component analysis, using sampling periods and algal densities (PCA‐1), indicated a heterogeneous phytoplankton assemblage, whereas PCA‐2, with algal densities and biochemical variables (soluble carbohydrates, Chl‐a and carotenoids), indicated that biochemical parameters could provide qualitative information about La Angostura phytoplankton community responses to quality of the sunlight.     

Feasibility studies on in‐situ biological treatment of drilling muds at an abandoned site in Sicily

The ongoing production and bioaccumulation of drilling fluids and muds has resulted in an increased concern for reducing the environmental impact of contaminated sites. The use of bioremediation as a treatment option is an important focus of research. Objectives of this study were: (i) to assess initial toxicity of drilling mud from an agricultural setting in Galliano, Sicily (Italy) and (ii) to develop a low intervention bioremediation approach to ensure that US and international soil/ground water quality standards have been met. Total petroleum hydrocarbon content of the drilling mud was reduced to 617.0 ± 176.0, 446.0 ± 195.0, and 533.0 ± 138.0 mg kg^?1, respectively, from 5000.0 ± 530.0 mg kg^?1 after treatment via conventional ex‐situ soil slurry reactor mixing (84.2–95% reduction). Using an in‐situ approach with bioreactors placed vertically into the zone of contamination, hydrocarbon concentrations went from 217.12 ± 43.38 and 149.68 ± 45.51 mg kg^?1 to 15.16 ± 3.35 and 34.27 ± 15.86 mg kg^?1 for control test beds (85.1 ± 11.2% reduction), and from 89.20 ± 67.42, 141.71 ± 64.80, and 197.87 ± 77.38 mg kg^?1 to 5.24 ± 6.15, 15.02 ± 10.20, and 9.65 ± 9.37 mg kg^?1 for experimental test beds (92.9 ± 3.0% reduction), respectively. Cadmium (Cd) and Selenium (Se) had exceeded Italian and state soil leachability standards (0.005 mg dm^?3 Cd) and (0.010, 0.050 mg dm^?3 Se), at the conclusion of the study. In‐situ treatment of drilling mud provided a framework for monitoring the fate of organic and residual metals toxicity in soils. Copyright © 2003 Society of Chemical Industry     

The influence of seasonality and duration of flooding on zooplankton in experimental billabongs

The regulation of Australian lowland rivers such as the River Murray has resulted in changes to the flooding characteristics of many associated wetlands. It has been suggested that these changes in flooding have changed the pattern of response of many wetland biota. The substantial variability in the size and shape of these wetlands makes limited field observations difficult to interpret. To overcome this variability 16 experimental billabongs were constructed in which factors that may cause changes to populations within billabongs could be manipulated. In this paper we report on experiments that test the hypothesis that changing the pattern of flooding alters the density and diversity of rotifers and microcrustaceans in billabongs. The experimental billabongs were sampled fortnightly for two years. During this time four flood events were imposed. Flooding of the experimental billabongs resulted in increased microcrustacean abundance. Rotifer abundance rarely increased following flooding. Changing the time of flooding did not modify this response. Observed changes in community structure following flooding result from changes in the relative densities of taxa already present rather than changes in the community composition. Copyright © 2002 John Wiley & Sons, Ltd.     

Experimental evolution gone wild

Because of their large population sizes and rapid cell division rates, marine microbes have, or can generate, ample variation to fuel evolution over a few weeks or months, and subsequently have the potential to evolve in response to global change. Here we measure evolution in the marine diatom Skeletonema marinoi evolved in a natural plankton community in CO₂-enriched mesocosms deployed in situ. Mesocosm enclosures are typically used to study how the species composition and biogeochemistry of marine communities respond to environmental shifts, but have not been used for experimental evolution to date. Using this approach, we detect a large evolutionary response to CO₂ enrichment in a focal marine diatom, where population growth rate increased by 1.3-fold in high CO₂-evolved lineages. This study opens an exciting new possibility of carrying out in situ evolution experiments to understand how marine microbial communities evolve in response to environmental change.     

Spatial and temporal variability in the structure of invertebrate assemblages in control stream mesocosms

Outdoor stream mesocosm studies conducted between 1992 and 1996 at two facilities enabled the investigation of structural variability in invertebrate assemblages within and between studies. Temporal variability of benthic invertebrate assemblages between eight replicate streams within a study was assessed in a 28-day mesocosm study without chemical treatment. Cluster analysis, non-metric multidimensional scaling, and principal component analysis each showed the untreated assemblages as structurally distinct groups on the three sampling days. The assemblages between the eight replicate streams showed >88% Bray-Curtis similarity at any one time during the study. In addition, pre-treatment data from a series of four studies conducted at one facility were used to examine structural variability in the starting benthic invertebrate assemblages between studies. Invertebrate assemblages were structurally distinct at the start of each mesocosm study conducted in different years at the same facility and the taxa responsible for differences in the assemblages were also different each year. The implications of temporal and spatial variability in benthic invertebrate assemblages within and between mesocosm studies with regards to species sensitivity and study repeatability should be considered when results of such studies are used in risk assessment.     

Experimental evidence for density‐dependent effects and the importance of algal production in determining population growth rates of riverine zooplankton

Food limitation effects on life history traits of lake zooplankton have been well documented but few studies have examined linkages between population growth rates and food resources in riverine environments. In rivers, allochthonous inputs of particulate organic matter may mitigate food limitation effects allowing density‐independent mechanisms associated with washout (discharge) and feeding interference (turbidity) to assume greater importance. We experimentally manipulated densities of commonly occurring riverine zooplankton (Bosmina longirostris and cyclopoid copepods) within 2000 l mesocosms containing ambient or algal‐enriched food resources. The experiment was repeated through time (July, August, September) to represent the range of zooplankton densities and food resource levels observed in the Ohio River during warm‐water, low‐flow conditions. High growth rates and low sensitivity to density‐dependent effects were observed during July when particulate organic carbon (POC) and chlorophyll concentrations were highest. Lower growth rates and stronger response to density‐dependent effects were observed during August and September experiments when POC and chlorophyll concentrations were lower. Direct manipulations of algal abundance resulted in higher growth rates when gains in chlorophyll were accompanied by increases in the edible size fraction (September experiment). Algal C concentrations were found to be a significant predictor of variation in population growth rates for Bosmina but not cyclopoids. Algal C concentrations in the Ohio River rarely fell below experimentally derived minimum food thresholds but were often below saturation thresholds suggesting that population growth rates were constrained by autochthonous food resources despite the prevalence of allochthonous carbon. Copyright © 2005 John Wiley & Sons, Ltd.     

Understanding the combined influence of fine sediment and glyphosate herbicide on stream periphyton communities

Pesticides and deposited fine sediment have independently been associated with changes in relative abundance and species richness in aquatic ecosystems, but the interplay between these two stressors in agricultural streams is poorly understood. A 28-day experiment in outdoor stream mesocosms examined the effects of four levels each of fine sediment coverage (0, 25, 75, 100%) and glyphosate-based herbicide concentration (0, 50, 200, 370 μg/L) on periphyton communities (algae and bacteria) in a fully factorial, repeated-measures design. Our aims were to determine whether (i) increased levels of sediment and glyphosate had individual and/or additive effects, (ii) increased sediment reduced the toxicity of glyphosate (antagonistic multiple stressor interaction), or (iii) sediment-adsorbed glyphosate prolonged the effects of exposure (synergistic interaction). We also assigned all algal taxa to three ecological guilds (low-profile, high-profile and motile growth forms) and separately determined their responses to the treatments. As individual stressors, sediment addition affected all algal community-level metrics, whereas glyphosate addition only affected algal community evenness. Bacterial taxon richness was unaffected by either stressor. In combination, however, significant overall sediment by glyphosate interactions were detected, demonstrating synergistic (algal evenness, high-profile and motile guilds) or antagonistic effects (low-profile guild). Our experiment underscores the importance of considering both structural and functional indicators, including algal guild representation, when assessing the mechanisms by which periphyton communities respond to multiple stressors.