Effect of microwave heating time on the gel properties of chicken myofibrillar proteins and their formation mechanism

The present work was conducted to illustrate the mechanism of gel formation of myofibrillar proteins (MPs) under different microwave heating times. The results showed that the denaturation enthalpy (ΔH) of the MPs significantly decreased when the heating time increased from 3 to 9 s and then completely disappeared as the heating time progressed, indicating that the MPs gradually denatured and subsequently aggregated with increasing heating time, which was further verified by the changes in the secondary structure, electrophoretic bands, and gel properties (e.g., water holding capacity and textural profiles) of the MPs. Microstructural images indicated that the MP gel formed under 12 s had the most compact network, indicating that extended microwave heating time could induce quality deterioration of MP gels. Moreover, the hydrophobic forces, electrostatic forces, and disulphide bonds of the MPs gradually intensified with increasing microwave heating time, suggesting that both non-covalent and covalent bonds could promote molecular denaturation and subsequent aggregation of MPs. In addition, correlation analysis revealed that the changes in the molecular conformation of MPs induced by different microwave heating times could effectively regulate the formation of MP gels and their related properties.     

爆破工程费用计算方法探讨

爆破工程具有周期短、自成完整体系、技术管理严格的特点,但在费用管理上需进一步规范与整顿。本文阐述了工程费用的构成、调整及爆破工程费用的计算方法。     

Photochemical Reactions in Supramolecular Assemblies of Gels: Dimerizations and Polymerizations via Pericyclic Reactions

Gel state reactions offer new direction for the reactivity of the organic molecules or metal‐organic materials upon photoirradiation with shorter reaction times and high yields compared to solid and solution states. The restricted molecular movement among the molecules in the soft solids control the stereoselectivity of the photoproducts in the gel state reactions. To date, most of the strategies based on self‐assembly have been demonstrated in the solid state, in particular for [2+2] reactions of olefins and polymerization reactions of diacetylenes via 1,4 addition. The soft materials are of emerging materials in recent days given their many applicative day‐to‐day aspects. This review gives a glimpse of recent reports on pericyclic reactions in the gel state that are designed based on the self‐assembly concept. Also it highlights how such reactions accompany the physical changes in the structure of the gels and stereo controlled products with high yields.     

Structures and properties of chicken myofibrillar protein gel induced by microwave heating

Structures and properties of myofibrillar protein gel prepared at different power (300–800 W) were evaluated. Amino acid analysis demonstrated that changes in microwave power did not alter primary structure of gel. However, an increase in microwave power could change higher structures of gel. As microwave power increased, α-helix content decreased and β-sheet content increased. Increased microwave power probably facilitated protein to unfold and expose the internal groups, causing surface hydrophobicity and the formation of disulphide bonds were enhanced, which indicated changes in tertiary and quaternary structures of protein. At 500 W, gel had the best ultrastructure where surface morphology, springiness and water holding capacity reached the optimum. Our findings suggested that microwave at an appropriate power (500 W) could change higher structures of myofibrillar protein gel to achieve desired processing and quality protein gel characteristics.     

Determination of molecular weight and molecular sizes of polymers by high temperature gel permeation chromatography with a static and dynamic laser light scattering detector

Flow-mode static and dynamic laser light scattering (SLS/DLS) studies of polymers, including polystyrene, polyethylene, polypropylene and poly(dimethylsiloxane) (PDMS), in 1,2,4-trichlorobenzene (TCB) at 150 °C were performed on a high temperature gel permeation chromatography (GPC) coupled with a SLS/DLS detector. Both absolute molecular weight (M) and molecular sizes (radius of gyration, R_g and hydrodynamic radius, R_h) of polymers eluting from the GPC columns were obtained simultaneously. The conformation of different polymers in TCB at 150 °C were discussed according to the scaling relationships between R_g, R_h and M and the ρ-ratio (ρ=R_g/R_h). Flow-mode DLS results of PDMS were verified by batch-mode DLS study of the same sample. The presented technique was proved to be a convenient and quick method to study the shape and conformation of polymers in solution at high temperature. However, the flow-mode DLS was only applicable for high molecular weight polymers with a higher refractive index increment such as PDMS.     

阴,阳离子聚合物地层内凝胶化改善水驱效果的研究

研究了一杆阴离子聚合物（Ａｃ５３０）和一种阳离子聚合物（Ｍｂ５８１）在水溶液中形成凝胶的条件和过程、凝胶化学结构、形态、稳定性和力学性能。在模拟地层的二维微观模型内观测了两种聚合物驱替渗流、相逼、形成凝胶、凝胶封堵大孔道的机理。在亲水填砂模型内测定了阴、阳离子聚合物凝胶体系降低渗透率的能力。     

超细水泥封堵技术

注水开发后期日益出现的地质问题及油套管的严重损坏,制约了油田的开发效果。针对河南油田注水开发中存在的上述问题,通过对超细水泥堵剂配方、堵剂性能、适应性等方面的研究,可实现对油井的高含水层、注水井的停注无用层、油水井套管外窜槽、穿孔、丝扣处的漏失等方面的封堵,取得了显著效果。自1998年8月-2001年12月在河南油田应用61井次,有效率85.3%,其中油井36井次,年度累计增油1.441×104t,降水7.6123×104m3;水井封堵25井次,累计增注14.32×104m3,控制无效注水16.95×104m3,创效益1800多万元,投入产出比达1:11.36,具有广泛的推广应用价值。     

Comparative study of the colorants monascus and cochineal used in the preparation of gels made with various gelling agents

The possibilities of using monascus to colour gels were studied by comparing it with cochineal. Gels were prepared with four gelling agents—carrageenan, gellan, gelatine and xanthan—and their colour was measured with a Hunter Lab colorimeter. The equations relating L^* and h with the concentrations of sugar [SU] and colorant [COL] were deduced, and it was found that [COL] had more influence on colour than [SU]. In general, for these four gelling agents the colour of monascus gels is orange-red and the colour of cochineal gels is purplish red. The gels made with xanthan were always the lightest, whether they were made with cochineal or with monascus.     

DST压恢法在大港油田的应用

非自喷井解释方法一直是困扰试井界的难题之一。自1995年底应用DKS2．0软件中DST压恢分析法先后处理了大港油田200多层实测资料以来，发现一部分井与常规分析法的处理结果完全不同。这些井多出现在均质无限大油藏，且其压力及压力导数曲线开口小、导数曲线驼峰较小。通过对DST压恢分析法在大港油田应用情况的分析，认为此方法适用于均质非自喷井，尤其是处理低渗且地层受污染井的结果比较符合地层的实际地质情况，其成果的应用对地层改造有一定的指导作用。     

内管注水泥固井工具的研究与分析

内管注水泥技术是针对大尺寸套管和表层套管固井的特点及特殊要求而采用的一种固井技术，具有省时、替量少等特点，在现场得到大量推广应用。内管注水泥器是内管固井技术的关键工具，其性能好坏直接影响到固井质量。对各种内管注水泥固井工具的结构及特点分别进行了分析研究，并就其发展方向提出建议。