Electrochemical Quantification of Neurotransmitters in Single Live Cell Vesicles Shows Exocytosis is Predominantly Partial

Exocytosis plays an essential role in the communication between cells in the nervous system. Understanding the regulation of neurotransmitter release during exocytosis and the amount of neurotransmitter content that is stored in vesicles is of importance, as it provides fundamental insights to understand how the brain works and how neurons elicit a certain behavior. In this minireview, we summarize recent progress in amperometric measurements for monitoring exocytosis in single cells and electrochemical cytometry measurements of vesicular neurotransmitter content in individual vesicles. Important steps have increased our understanding of the different mechanisms of exocytosis. Increasing evidence is firmly establishing that partial release is the primary mechanism of release in multiple cell types.     

Knowledge-Based Virtual Modeling and Simulation of Manufacturing Processes for Industry 4.0

Abstract

Industry 4.0 aims at providing a digital representation of a production landscape, but the challenges in building, maintaining, optimizing, and evolving digital models in inter-organizational production chains have not been identified yet in a systematic manner. In this paper, various Industry 4.0 research and technical challenges are addressed, and their present scenario is discussed. Moreover, in this article, the novel concept of developing experience-based virtual models of engineering entities, process, and the factory is presented. These models of production units, processes, and procedures are accomplished by virtual engineering object (VEO), virtual engineering process (VEP), and virtual engineering factory (VEF), using the knowledge representation technique of Decisional DNA. This blend of the virtual and physical domains permits monitoring of systems and analysis of data to foresee problems before they occur, develop new opportunities, prevent downtime, and even plan for the future by using simulations. Furthermore, the proposed virtual model concept not only has the capability of Query Processing and Data Integration for Industrial Data but also real-time visualization of data stream processing.     

基于开源技术的城市地理信息平台构建方法研究

摘 要：针对城市地理信息平台构建过程中存在的数据获取困难、研发成本较高等问题， 引入开源技术，对基于开源数据、开源开发平台与开源工具的城市地理信息平台构建技术进行 研究。所提出的城市地理信息平台由数据模块与可视化模块 2 部分组成。为构建数据模块，由 开源地理信息项目(OSM)获取了建筑与区划数据，并设计了建筑合并算法以提升数据质量。建 筑数据随之基于行政区划分级存储，通过由 ASP. NET 构建的数据接口提供给具体应用。可视 化模块则基于开源地理信息系统(GIS)项目 Cesium 实现，并设计了完善的用户界面。以北京市 为例，完成了原型系统的开发与测试，证实了该技术体系的可行性，通过开源技术降低平台研 发成本，推进城市地理信息平台的研究与应用。     

大牛地气田气井无阻流量的确定方法研究

大牛地气田气井试井大多采用一点法，由于气层渗透性差，测试时间短，流压很难达到稳定，计算的无阻流量往往偏高，且无法获得正确的产能方程。通过气田多口井修正等时试井数据进行统计分析，获得了一点法试井在不同生产时间下计算无阻流量的经验公式；利用气田修正等时试井的二项式方程系数曰，计算惯性阻力系数并经过曲线回归处理获得了经验关系式，很好地解决了一点法试井计算产能方程的问题。     

农村饮水解困工程持续利用的探讨

分析了尧都区农村饮水工程存在的问题，阐述了农村饮水解困工程持续利用的管理措施，提出建立新的农村供水市场理念。     

有线电视工程建设流程再造设计

从理论与实际工作相结合的角度出发,以淄博广电青鸟公司为研究对象,对有线电视网络工程建设流程的现状、存在的问题和原因进行细致深入分析,然后按照提出的再造方案的设计原则,设计了工程建设流程的再造方案,并对新旧流程进行比较。     

电信企业信息化统一模型构建策略

电信企业信息化的实质就是赋予电信企业精细化的企业资源整合能力，本以此认识为出发点，通过定义基本的信息化业务元素，需求参数和基本信息处理机制，构建出带有行业共性的电信企业信息化统一模型，该模型针对目前电信企业信息化中常见的和潜在的问题提供了有效的解决措施。     

深井长裸眼跨隔测试新工艺的应用

为减少勘探费用，缩短施工周期，取全取准各项地质资料，采取中途测试工艺。随着深井中途测试不断增多，特别是跨隔支撑测试具有一定施工风险和难度，要求测试质量不断提高。结合油田生产实际，合理选配了地层测试工具仪表，不断改进测试管串和优化施工设计，经盐22井中途跨隔测试应用，获得成功。     

车古201潜山油藏完井方式研究

研究了国内外潜山油藏完井方式应用及其使用条件、井身结构的应用 ,针对车古 2 0 1油藏特点 ,论证了其对裸眼完井、筛管完井、尾管射孔完井、管外封隔器完井的适应性 ,通过对比研究 ,从更好地满足油藏工程、采油工艺、增产措施出发 ,选择出车古 2 0 1潜山油藏最合理的完井方式为尾管注水泥射孔完井 ,并给出了应采用的井身结构