Inhibitory Monoclonal Antibodies and Their Recombinant Derivatives Targeting Surface-Exposed Carbonic Anhydrase XII on Cancer Cells

Monoclonal and recombinant antibodies are widely used for the diagnostics and therapy of cancer. They are generated to interact with cell surface proteins which are usually involved in the development and progression of cancer. Carbonic anhydrase XII (CA XII) contributes to the survival of tumors under hypoxic conditions thus is considered a candidate target for antibody-based therapy. In this study, we have generated a novel collection of monoclonal antibodies (MAbs) against the recombinant extracellular domain of CA XII produced in HEK-293 cells. Eighteen out of 24 MAbs were reactive with cellular CA XII on the surface of live kidney and lung cancer cells as determined by flow cytometry. One MAb 14D6 also inhibited the enzymatic activity of recombinant CA XII as measured by the stopped-flow assay. MAb 14D6 showed the migrastatic effect on human lung carcinoma A549 and renal carcinoma A498 cell lines in a ‘wound healing’ assay. It did not reduce the growth of multicellular lung and renal cancer spheroids but reduced the cell viability by the ATP Bioluminescence assay. Epitope mapping revealed the surface-exposed amino acid sequence (35-FGPDGENS-42) close to the catalytic center of CA XII recognized by the MAb 14D6. The variable regions of the heavy and light chains of MAb 14D6 were sequenced and their complementarity-determining regions were defined. The obtained variable sequences were used to generate recombinant antibodies in two formats: single-chain fragment variable (scFv) expressed in E. coli and scFv fused to human IgG1 Fc fragment (scFv-Fc) expressed in Chinese Hamster Ovary (CHO) cells. Both recombinant antibodies maintained the same specificity for CA XII as the parental MAb 14D6. The novel antibodies may represent promising tools for CA XII-related cancer research and immunotherapy.     

钨合金圆筒爆破试验破片分布的统计分析

采用圆筒爆破试验方法研究了易碎型钨合金的破片分布特征,并与93W合金的结果进行了对比.对破片的数量和质量分布特征进行了统计分析,发现易碎型钨合金的破片率明显高于93W合金,而且破片的均匀性也明显好于93W合金,但易碎钨合金的破片回收率低于93W合金,而且受装药量的影响较大.     

Estimating and eliminating redundant data transfers over the web: a fragment based approach

Redundant data transfers over the Web, can be mainly attributed to the repeated transfers of unchanged data. Web caches and Web proxies are some of the solutions that have been proposed, to deal with the issue of redundant data transfers. In this paper we focus on the efficient estimation and reduction of redundant data transfers over the Web. We first prove that a vast amount of redundant data is transferred in Web pages that are considered to carry fresh data. We show this by following an approach based on Web page fragmentation and manipulation. Web pages are broken down to fragments, based on specific criteria. We then deal with these fragments as independent constructors of the Web page and study their change patterns independently and in the context of the whole Web page. After the fragmentation process, we propose solutions for dealing with redundant data transfers. This paper has been based on our previous work on ‘Web Components’ but also on related work by other researchers. It utilises a proxy based, client/server architecture, and imposes changes to the algorithms executed on the Proxy server and on clients. We show that our proposed solution can considerably reduce the amount of redundant data transferred on the Web. Copyright © 2004 John Wiley & Sons, Ltd.     

破片式战斗部优化设计技术研究

侧重研究了破片式战斗部装药外形的优化设计技术。提出了装药外形与破片飞散特性（如破片飞散角，分布带宽等）之间的数学描述；建立了圆弧和对数螺线装药外形母线与战斗部其他设计参数间的数学模型。在假定约束条件下，优化设计了破片了聚焦式战斗部的结构参数；静爆试验结果表明，破片分布与理论计算吻合较好。     

小型预制桩基础承载特性数值模拟研究

本文建立硬化土本构模型,针对江苏省软土地区的土质条件选取数值计算参数,通过PLAXIS 2D软件对小型预制桩在的单桩水平受荷及竖向受荷进行数值模拟,得到桩身弯矩沿桩长的分布以及荷载-位移曲线,分析小型预制桩基础桩身弯矩的分布特点,并探求其在水平及竖向荷载作用下的承载特性,进而能够为小型预制桩基础工程设计以及计算方面提供一定的参考依据。     

4-(α-吡啶甲酰基)-PMP的合成与谱学特性研究

合成了新吡唑啉酮类试剂1-苯基-3-甲基-4-(α-吡啶甲酰基)-吡唑啉-5-酮(简称4-(α-吡啶甲酰基)-PMP,下同)。在表征分子结构的同时,对其红外、紫外吸收光谱,核磁共振氢谱、碳谱,质谱等谱学特性和质谱裂解规律进行了分析与研究。     

基于文档文字特征的碎纸机碎片拼接算法

针对经碎纸机粉碎的文档碎片,提出拼接复原的模型并研制了相应的算法。首先研究文档碎片边界像素特征,据此提出碎片横向拼接的两种算法;然后,研究行高、字体间间距以及字体大小等文本特征,据此提出针对经横向拼接形成的水平残条的纵向拼接算法。最后根据算法编写Matlab程序,并进行实验,结果表明该算法效果良好。     

Viscoelastic and Processing Effects on the Fiber-Matrix Interphase Strength. Part I. The Effects of Loading Rate, Test Temperature, Fiber Sizing and Global Strain Level

The effects of loading rate, fiber sizing, test temperature and global strain level on the adhesion strength between carbon fibers and a thermosetting epoxy (Epon 815) are studied using the single fiber fragmentation test procedure. Analytical methodology describing the viscoelastic behavior observed is also presented. The possibility of rate-temperature-interphase thickness superposition for the interfacial strength function is illustrated based on the analytical models discussed. Experimental data are discussed using Weibull statistics and also presented in the form of percent relative frequency histograms for the fiber fragments in a collective fashion. The use of histograms allows for interpretation of the skewness in the data population.     

基于BIM技术的装配式建筑设计阶段协同度评估

为评估BIM技术对装配式建筑设计阶段协同能力的影响程度,基于协同论序参量原理,把软件协同、专业协同作为协同度测度模型的一级指标,在一级指标下选取22个序参量分量指标构建协同度模型。结合具体案例,以装配式建筑设计阶段为研究对象,运用专家打分法将测量指标进行更明确的量化,并借助功效函数对构建的指标体系的协同能力进行评估计算。结果表明：由于BIM软件之间数据共享与转换格式的不统一,仍出现了信息丢失、传递效率低的现象;专业协同与理想状态有一定的差距,BIM技术有待完善。     

悬臂梁段不同拼接方式下延性节点静力性能分析

为了解决装配式钢结构的抗震问题,提出3种适用于模块化装配式钢框架的带Z字形悬臂梁段拼接的梁柱节点,通过拼接区的滑移或者削弱梁段的塑性变形实现节点良好的延性性能.为减少强震作用对梁柱连接焊缝的破坏,对3种不同的节点提出了各自的抗震设计要求.在对3组试件进行足尺静力试验的基础上,对节点进行了单调加载有限元分析.获得了节点的弯矩-转角曲线以及节点的破坏模式,并和试验结果进行了对比,得到了接触面摩擦力和螺栓拉力的变化规律.通过推导的简化计算公式对节点的初始转动刚度、滑移荷载和屈服荷载进行了计算.结果表明:节点静力加载的破坏模式为靠近拼接区域的悬臂梁下翼缘或者削弱梁段单独或者同时发生较大的局部屈曲变形,节点在破坏前经历了充分的塑性变形,属于延性破坏,3种试件都有良好的延性性能和塑性转动能力.节点简化计算公式得到的结果和试验结果吻合良好.