Evaluating Cell Disruption Strategies for Aqueous Lipid Extraction from Oleaginous Scenedesmus obliquus at High Solid Loadings

Among various renewable energy sources, the production of biofuels derived from algal lipids holds bright prospects. One of the major roadblocks in the successful commercialization of microalgal biofuels is the existing energy‐intensive lipid extraction. In the present investigation, an attempt is made to assess aqueous lipid extraction strategies from oleaginous Scenedesmus obliquus at a high solid loading of 15% (w/v). In this study, four surfactants and five enzymes are evaluated for cell disruption of S. obliquus. It is the first report citing cetyl pyridinium bromide as the most suitable cationic detergent for surfactant‐assisted extraction, with a lipid recovery as high as 31.4%. However, during the evaluation of enzyme‐based cell disruption, neutral protease emerges as the best biocatalyst resulting in a lipid recovery of ≈75%. Total lipid extraction is accomplished using a two solvent system comprising of water‐immiscible ethyl acetate, followed by chloroform addition. The study revalidates the fact that the biochemical composition of Scenedesmus sp. plays a vital role while identifying and formulating an efficient and green process for microalgal cell disruption for enhanced lipid extraction under aqueous conditions. Practical Applications: The results of the present study demonstrate that if the biochemical composition of any oleaginous algal cell wall is known, aqueous enzymatic lipid extraction can be employed rather than taking up the conventional route of drying followed by Soxhlet extraction. The combination of using the cheap sources of enzymes and water‐immiscible green solvents like ethyl acetate can be lucrative downstream procedures for the lipid recovery from wet algal biomass when compared to traditional procedures.     

提高数控切割机的切割能力

通过对现有数控切割机加工能力的分析,结合单位生产实际需要,对数控切割机的有效切割长度进行加长;采用CCAP编程软件进行下料件的程序编制,减少生产时的辅助时间,提高生产效率,进而提高设备利用率。     

一种集成式CMC一体机在HFC接入网中的应用

C-DOCSIS相关技术标准的发布极大地促进了其在HFC网络中的部署。但C-DOCSIS系统头端设备覆盖规模和光纤深化的趋势之间存在矛盾。本文从带宽模型、带宽容量、频率资源三个方面进行分析，提出了一种集成式的CMC一体机的设备架构，兼顾了覆盖规模和投资成本，为C-DOCSIS在HFC网络中的大规模部署提供了有效的解决方案。     

C-DOCSIS技术应用和发展展望

本文详细介绍了C-DOCSIS技术的架构和性能特点,并分析了目前C-DOCSIS技术在应用过程中面临的不同方面的问题。并在此基础上,根据DOCSIS技术的最新发展,概括了下一代C-DOCSIS技术需要重点投入资源开展研究的技术方向。