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排序方式: 共有137条查询结果,搜索用时 46 毫秒
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Xiang Jim; Sha Yuhua; Prasad Lata; Delbaere Louis T.J. 《Protein engineering, design & selection : PEDS》1996,9(6):539-543
Structural analysis derived from the crystallographic studyof the chimeric B72.3 antibody illustrated some major atomicinteractions between complementarity determining region (CDR)residues. For example, hydrogen bonds are formed between H35/H95,L50/H97, H53/H55 and H96/L96 respectively. These CDR residuesmay play important roles in the B72.3TAG72 (antibody-antigen)interaction either by direct interaction with the TAG72 antigenor by maintaining a CDR loop conformation through atomic interactionsbetween CDR residues. In order to confirm these assumptions,we altered these CDR residues by site-directed mutagenesis anddetermined binding affinities of these mutant chimeric antibodiesfor the TAG72 antigen in a solid-phase radioimmunoassay. Wefound that H55, H95, H97 and L96 are important CDR residuesfor the B72.3TAG72 interaction. Single amino acid substitutionsof aspartic acid and serine by alanine at H55 of CDR2 and atH95 of CDR3 respectively and of tyrosine by phenylalanine atH97 and L96 of CDR3, significantly reduced the binding affinityfor the TAG72 antigen by 20-, 8-, 16- and 45-fold respectively.Therefore, this study reveals some of the requirements for maintainingthe integrity of the B72.3 antibody combining sites. 相似文献
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为了降低高速串行接口的时钟数据恢复(CDR)电路的功耗,在研究、分析现有时钟数据恢复结构的基础上,提出了一种新的时钟数据鉴相算法及其电路实现方法。新的电路设计仅使用一个高速采样时钟,比传统的鉴相电路减少一半的采样率,从而减少了前端采样模块的功耗。该鉴相算法采用统计方法减小鉴相时钟的噪声,进而达到很低的误码率。该鉴相算法可使用数字综合的方法实现,工作在较低的频率下,这样便于迁移到不同的工艺中。整个电路使用40nm工艺实现,实际芯片测试数据表明,使用该电路的接收端可以稳定工作在13Gb/s的速率下,功耗达到0.83p J/bit,误码率低于10E-12。 相似文献
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《Food Control》2015
Tree nut allergies are considered an important health issue in developed countries. To comply with the regulations on food labeling, reliable allergen detection methods are required. In this work we isolated almond-specific recombinant antibody fragments (scFv) from a commercial phage display library bypassing the use of live animals, hence being consistent with the latest policies on animal welfare. To this end an iterative selection procedure employing the Tomlinson I phage display library and a crude almond protein extract was carried out. Two different almond-specific scFv (named PD1F6 and PD2C9) were isolated after two rounds of biopanning, and an indirect phage ELISA was implemented to detect the presence of almond protein in foodstuffs. The isolated scFvs demonstrated to be highly specific and allowed detection of 40 ng mL−1 and 100 ng mL−1 of raw and roasted almond protein, respectively. The practical detection limit of the assay in almond spiked food products was 0.1 mg g−1 (110–120 ppm). The developed indirect phage ELISA was validated by analysis of 92 commercial food products, showing good correlation with the results obtained by a previously developed real-time PCR method for the detection of almond in foodstuffs. The selected phage clones can be affinity maturated to improve their sensitivity and genetically engineered to be employed in different assay formats. 相似文献
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Anil K. Mathur Rahul Chandrajit B. Majumder Shashi B. Gautam Ian J. Mcnaught 《The International journal of environmental studies》2013,70(3):475-489
In this study, the overall performance of a biofilter was evaluated in terms of its elimination capacity by using 3-D mesh techniques. The overall results indicate that the agreement between experimental data and model predictions is excellent for benzene, toluene, ethylbenzene and o-xylene (BTEX). In this study, the maximum removal rate (r max) values for BTEX were 0.0117, 0.0126, 0.0081 and 0.0146 g m–3 h–1, and the half-saturation constant (KS ) values were calculated to be 0.269, 0.297, 0.156 and 0.394 g m–3, respectively. For this system, the coefficients of determination (r 2) of BTEX compounds were greater than 0.97. The BTEX concentration profiles along the depth were also determined using a convection–diffusion reactor (CDR) model. The sums of squares of the errors (SSEs) of BTEX were 0.0078, 0.0059, 0.0129 and 0.0269, respectively, with r 2 values greater than 0.99 for all four compounds at low concentrations. 相似文献
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一种快速同步的时钟数据恢复电路的设计实现 总被引:4,自引:1,他引:4
时钟数据恢复(CDR)电路是通信传输设备中的重要部分,对于突发式的接收,基于锁相环的传统的CDR往往不能满足其快速同步的要求.对此,文章采用过采样方式基于FPGA设计实现了一种全数字化的155.52Mb/s下的CDR电路.理论分析、仿真和实验测试结果表明,该CDR电路可以有效地对相位变化实现快速同步,有很大的捕捉范围,且系统较锁相环便于集成. 相似文献
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媒体网关控制协议是NGN网络中一个重要的协议。呼叫数据记录合成属于网络测试仪的基础功能部分。文中主要研究NGN网络测试仪中MGCP协议的监测软件实现方法,参照标准流程,提出了MGCP协议的呼叫数据记录合成方案。 相似文献
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针对预付费及后付费业务融合如何实现,提出并分析了融合支撑系统的组成、功能结构、流程,对融合支撑中涉及到的重要问题和对现有系统的影响进行了探讨、分析并提出了建议. 相似文献