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1.
An engineered variant of the yeast Pichia pastoris was used to produce the potential malaria vaccine D1M1. This paper describes the implementation of an expanded bed adsorption chromatography step into a sequential cultivation process of the yeast in order to link protein expression, cell release, and product capture of the secreted protein in a fully automated plant. The process is operated with a semi-continuous strategy for an integrated production. Process improvements have been achieved using design of experiments. Resulting purities of product achieved up to 87% with recoveries of 51% in a single downstream operation. Furthermore, a multivariate analysis of historical batch data was used to develop a golden batch model for process monitoring. 相似文献
2.
This article presents a new approach for solving the Optimal Control Problem (OCP) of linear time-delay systems with a quadratic cost functional. The proposed method can also be used for designing optimal control time-delay systems with disturbance. In this study, the Variational Iteration Method (VIM) is employed to convert the original Time-Delay Optimal Control Problem (TDOCP) into a sequence of nonhomogeneous linear two-point boundary value problems (TPBVPs). The optimal control law obtained consists of an accurate linear feedback term and a nonlinear compensation term which is the limit of an adjoint vector sequence. The feedback term is determined by solving Riccati matrix differential equation. By using the finite-step iteration of a nonlinear compensation sequence, we can obtain a suboptimal control law. Finally, Illustrative examples are included to demonstrate the validity and applicability of the technique. 相似文献
3.
We extend the definition of the classical Jacobi polynomials withindexes α, β>−1 to allow α and/or β to be negative integers. We show that the generalized Jacobi polynomials, with indexes corresponding to the number of boundary conditions in a given partial differential equation, are the natural basis functions for the spectral approximation of this partial differential equation. Moreover, the use of generalized Jacobi polynomials leads to much simplified analysis, more precise error estimates and well conditioned algorithms.Mathematics subject classification 1991. 65N35, 65N22, 65F05, 35J05 相似文献
4.
Kelson C. Lopes Flvia S. Pereira Regiane C. M. U. Araújo Mozart N. Ramos Roy E. Bruns 《Chemometrics and Intelligent Laboratory Systems》2004,70(2):157-163
Factorial design and principal component models are used to determine how ab initio H-bond stretching frequencies depend on characteristics of the molecular orbital wave functions of acetylene–HX, ethylene–HX and cyclopropane–HX π-type hydrogen complexes with X=F, Cl, CN, NC and CCH. The results obtained for the three sets of complexes show that factorial design and principal component analyses complement each other. Factorial design calculations clearly show that these frequencies are affected mostly by inclusion of electron correlation on the calculation level. On average, their values are increased by about 25 cm−1 due to a change from the Hartree–Fock (HF) to Möller–Plesset 2 (MP2) level. Valence, diffuse and polarization main effects as well as valence–diffuse, diffuse–correlation and polarization–correlation interaction effects are also important to better describe a factorial model to the H-bond stretching frequencies of these hydrogen complexes. This simplified model has been successful in reproducing the complete ab initio results, which correspond to two hundred and forty calculations. Principal component analyses applied only to hydrogen-bonded complexes whose experimental frequencies are known, has revealed that the six-dimensional original space can be accurately represented by a bidimensional space defined by two principal components. Its graphical representation reveals that the experimental intermolecular stretching frequencies are in closest agreement with the MP2/6–31+G and MP2/6–311+G ab initio results. 相似文献
5.
I. Budić-Leto T. Lovrić J. Gajdoš Kljusurić I. Pezo U. Vrhovšek 《European Food Research and Technology》2006,222(3-4):397-402
Identification of anthocyanins in the wine made of the Croatian autochthonous grape variety of Babić (Vitis vinifera L.) was carried out and their profile was determined by means of high-performance liquid chromatography with diode array
and mass spectrometric detection. Dependence of anthocyanins content and profile on maceration treatment conditions was investigated.
Statistically significant differences of anthocyanins concentration in wines Babić produced by various maceration treatments
were confirmed by the use of multivariate analysis of variances. The investigation results indicated that the maceration temperature
exerts higher influence on anthocyanins concentration than the duration of maceration. In addition, on the basis of anthocyanin
composition and using different multivariate statistical analyses, differentiation of wines Babić according to maceration
treatments was procured. 相似文献
6.
现代电子工业Cpk评价中的特殊问题 总被引:2,自引:0,他引:2
现代电子工业生产具有许多新的特点,如果不针对具体情况,均采用传统工业生产中广泛使用的常规方法计算其工序能力指数,评价生产工艺水平,将可能导致错误的结论。总结了电子工业生产中工艺参数的典型特点,并讨论了工序能力指数的正确计算方法。 相似文献
7.
The influence of raw material characteristics on the industrial pelletizing process and pellet quality 总被引:1,自引:0,他引:1
Mehrdad Arshadi Rolf Gref Paul Geladi Sten-Axel Dahlqvist Torbjörn Lestander 《Fuel Processing Technology》2008
Industrial pelletizing of sawdust was carried out as a designed experiment in the factors: sawdust moisture content, fractions of fresh pine, stored pine and spruce. The process parameters and response variables were energy consumption, pellet flow rate, pellet bulk density, durability and moisture content. The final data consisted of twelve industrial scale runs. Because of the many response variables, data evaluation was by principal component analysis of a 12 × 9 data matrix. The two principal component model showed a clustering of samples, with a good reproducibility of the center points. It also showed a positive correlation of energy consumption, bulk density and durability all negatively correlated to flow rate and moisture content. The stored pine was more related to high durability and bulk density. The role of the spruce fraction was unclear. The design matrix, augmented with the process parameters was a 12 × 6 matrix. Partial least squares regression showed excellent results for pellet moisture content and bulk density. The model for durability was promising. A 12 × 21 data matrix of fatty- and resin acid concentrations measured by GC–MS showed the differences between fresh and stored pine very clearly. The influence of the spruce fraction was less clear. However, the influence of the fatty- and resin acids on the pelletizing process could not be confirmed, indicating that other differences between fresh and stored pine sawdust have to be investigated. This work shows that it is possible to design the pelletizing process for moderate energy consumption and high pellet quality. 相似文献
8.
Chemometrics has enjoyed tremendous success in the areas related to calibration of spectrometers and spectroscopy-based measurements. These chemometric-based spectrometers have been widely applied for process monitoring and quality assurance. However, chemometrics has the potential to revolutionize the very intellectual roots of problem solving. Are there barriers to a more rapid proliferation of chemometric-based thinking, particularly in industry? What are the potential effects of chemometrics technology and the New Network Economy (NNE) working in concert? Who will be the winners in the race for faster, better, cheaper systems and products? These questions are reviewed in terms of the principles of the NNE and in the promise of chemometrics for industry. What then is the state of multivariate thinking in industry? Several powerful principles are derived from an evaluation of the NNE and chemometrics which could allow chemometrics to proliferate much more rapidly as a key general problem-solving tool. 相似文献
9.
10.
Claus Bahlmann Author Vitae 《Pattern recognition》2006,39(1):115-125
The selection of valuable features is crucial in pattern recognition. In this paper we deal with the issue that part of features originate from directional instead of common linear data. Both for directional and linear data a theory for a statistical modeling exists. However, none of these theories gives an integrated solution to problems, where linear and directional variables are to be combined in a single, multivariate probability density function. We describe a general approach for a unified statistical modeling, given the constraint that variances of the circular variables are small. The method is practically evaluated in the context of our online handwriting recognition system frog on hand and the so-called tangent slope angle feature. Recognition results are compared with two alternative modeling approaches. The proposed solution gives significant improvements in recognition accuracy, computational speed and memory requirements. 相似文献