排序方式: 共有9条查询结果,搜索用时 15 毫秒
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采用磁控溅射、光刻、离子束刻蚀和剥离工艺等工艺和方法,制备了多层膜结构的巨磁阻(GMR)生物传感器件,并利用此种传感器来检测甲胎蛋白。在传感器表面通过生物处理固定甲胎蛋白单克隆抗体(McAb1)作为探针,以捕获目标抗原———甲胎蛋白。用直径1μm的超顺磁磁珠标记目标抗原。当传感器表面抗体将目标抗原捕获后,磁珠标记即被固定在GMR传感器的表面。垂直于传感器表面施加230 Oe(1 Am-1=4π×10-3 Oe)的磁场,即可检测到由磁珠产生的信号。本实验对质量浓度为1 ng/mL的甲胎蛋白进行了检测,得到了信号为0.29~0.34Ω的电阻变化值。此种检测方法可用于诊断原发性肝癌。 相似文献
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微小RNA(miRNA)是一类新发现的长度为21-25个核苷酸的RNA,它在转录后水平调节靶基因表达.已有研究表明,miRNA在发育、细胞增殖、凋亡、脂类代谢、激素分泌及肿瘤发生等多种生理和病理过程中发挥重要作用.针对miRNA的检测技术的研究主要包括2大类:一是以传统实验技术方法为基础建立起来miRNA特有的技术方法,二是已成熟应用的生物传感技术.综述了2大类miRNA的检测技术,并对现有的检测技术的特点进行分析. 相似文献
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P. Venkatesan 《Journal of Experimental Nanoscience》2014,9(3):293-298
Most of the hydrogen peroxide (H2O2) bio-sensors developed till date are based on enzymes and proteins causing them to have a limited lifetime. Moreover, complex procedures are followed for sensor fabrication. Therefore, an inorganic material-based sensor, with a simple design and longer shelf life is highly desirable. In this work, surfactant-metal (gold and silver) nanoparticles are prepared in aqueous solutions containing cetyltrimethylammonium bromide. The particle sizes of the metal nanoparticles obtained are characterised by UV–Vis, HRTEM, X-ray diffraction and FTIR; the average sizes of gold and silver nanoparticles are 8 and 10?±?0.2?nm, respectively. The nanoparticles are tested for H2O2 detection. The sensor is characterised and tested using samples from M to mM H2O2 range and a linear response is observed. Low-detection limits and high sensitivity are some of the advantages of this work. Same principle could be extended for the detection of other substrates as well. 相似文献
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