Organic Semiconducting Luminophores for Near-Infrared Afterglow,Chemiluminescence, and Bioluminescence Imaging

Optical imaging has played a pivotal role in deciphering in vivo bioinformatics but is limited by shallow penetration depth and poor imaging performance owing to interfering tissue autofluorescence induced by concurrent photoexcitation. The emergence of near-infrared (NIR) self-luminescence imaging independent of real-time irradiation has timely addressed these problems. There are two main kinds of self-luminescent agents, namely inorganic and organic luminophores. Inorganic luminophores usually suffer from long-term biotoxicity concerns resulting from potential heavy-metal ions leakage and nonbiodegradability, which hinders their further translational application. In contrast, organic luminophores, especially organic semiconducting luminophores (OSLs) with good biodegradable potential, tunable design, and outstanding optical properties, are preferred in biological applications. This review summarizes the recent progress of OSLs used in NIR afterglow, chemiluminescence, and bioluminescence imaging. Molecular manipulation and nanoengineering approaches of OSLs are discussed, with emphasis on strategies that can extend the emission wavelength from visible to NIR range and amplify luminescence signals. This review concludes with a discussion of current challenges and possible solutions of OSLs in the self-luminescence field.     

Radical scavenging activity of canola hull phenolics

Possible use of canola hulls as a source of natural anti-oxidants was explored. Cyclone canola hulls were extracted with methanol (30 to 80%, vol/vol) and acetone (30 to 80%, vol/vol). The free radical-scavenging activity of phenolic extracts so prepared was evaluated using the 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radical ion (ABTS^o−), 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical, and chemiluminescence assays. The total content of phenolics in prepared extracts from canola hulls ranged from 15 to 136 mg sinapic acid equivalents per gram of extract. Higher levels of condensed tannins were detected in the acetone extracts than in the corresponding methanolic counterparts. Seventy and 80% (vol/vol) acetone extracts displayed markedly stronger antioxidant activity than any of the other extracts investigated. Statistically significant linear correlations were found between TEAC (Trolox equivalent antioxidant capacity) values (expressed in mM of Trolox equivalents per gram of extract) and total pehnolics, TEAC and total condensed tannins (i.e., determined using the modified vanillin and pronthocyanidin assays), as well as TEAC and protein precipitation activity of phenolic extracts (i.e., measured using the dye-labeled assay). The antioxidant activities of extracts as determined by the ABTS^o− radical ion assay correlated highly with those of the chemiluminescence and DPPH radical assays.     

药物抗氧化作用对DNA发光动力学行为的影响

本文应用ＣｕＳＯ４－Ｐｈｅｎ－Ｖｃ－Ｈ２Ｏ２－ＤＮＡ化学发光体系观察药物对发光动力学行为的影响，研究了九种药物对ＤＮＡ损伤的保护作用，并对其药物结构对抗氧化能力的影响进行了分析。发现药物在该系统中的抗氧化作用有三种类型，提示它们可能在体内不同的反应过程中起不同的作用。在该系统中具有抑制ＤＮＡ损伤发光能力的药物，按其作用强弱依次为阿魏酸、丹参素、芦丁，茶多酚、ＳＯＤ、、丹参酮Ⅱ－Ａ，丹皮酚，五味子乙     

无机片状微晶对化学光源发光效率的影响研究

以双（2,4,5-三氯-6-正丁烷氧羰基苯基）草酸酯（简称CPPO）和红色荧光剂罗丹明B组成的过氧草酸酯类化学发光体系为研究对象,从粒径、用量和种类的角度讨论了分散剂云母等对体系发光效率的影响．结果表明,添加30μm云母50mg后,体系的发光寿命、强度均显著增加,发光效率较高。     

化学发光材料双(2,4,6-三氯苯基)草酸酯的合成

草酸酯是过氧草酸酯类化学发光所必需的一种要素化合物，作者合成了一种能提供高强度化学发光的草酸酯即双（２，４，６ 三氯苯基）草酸酯，并以质谱验证了结构。     

钌(Ⅱ)-Ⅲ(1,10-菲咯啉)-KMnO_4化学发光体系测定硫脲

分别考察了钌(Ⅱ)-Ⅲ(1,10-菲咯啉)和硫脲在KMnO4、KBrO3、K2S2O8、I2、KIO4、 Ce(SO4)2存在下的化学发光反应情况,发现只有KMnO4能氧化硫脲和钌(Ⅱ)-Ⅲ(1,10-菲咯啉) 而发光。体系的化学发光强度与硫脲的浓度在2．0×10~(-8)～1．0×10~(-5)mol·L~(-1)范围内呈线性关系,方法的检出限为2．0×10~(-8)mol·L~(-1),平行测定10次相对标准偏差为1．1％。该法用于分析测定白葡萄酒中的硫脲,平均回收率为96．3％～103％获得满意结果。     

漂烫前后蔬菜总体抗氧化性的研究

在本研究中,对常见的六种蔬菜进行漂烫处理,采用流动注射化学发光法评价了六种蔬菜漂烫前后的总体抗氧化活性,为人体实际抗氧化物质摄入量及相关研究提供参考。在最优的条件下,测定结果表明:西红柿和白菜漂烫后总体抗氧化性降低;白萝卜、大蒜、洋葱漂烫后总体抗氧化性增强;胡萝卜经漂烫处理后总体抗氧化性先增强后降低。     

Near‐Infrared Intraoperative Chemiluminescence Imaging

Intraoperative imaging technologies recently entered the operating room, and their implementation is revolutionizing how physicians plan, monitor, and perform surgical interventions. In this work, we present a novel surgical imaging reporter system: intraoperative chemiluminescence imaging (ICI). To this end, we have leveraged the ability of a chemiluminescent metal complex to generate near‐infrared light upon exposure to an aqueous solution of Ce⁴⁺ in the presence of reducing tissue or blood components. An optical camera spatially resolves the resulting photon flux. We describe the construction and application of a prototype imaging setup, which achieves a detection limit as low as 6.9 pmol cm^?2 of the transition‐metal‐based ICI agent. As a proof of concept, we use ICI for the in vivo detection of our transition metal tracer following both systemic and subdermal injections. The very high signal‐to‐noise ratios make ICI an interesting candidate for the development of new intraoperative imaging technologies.     

荧光化学发光法研究吴茱萸精油的抗氧化作用

用精制的吴茱萸挥发油进行抗氧化研究,用荧光化学发光法测定了吴茱萸精油对活性氧自由基的清除作用.实验中采用了 Vc 作为标准对照物,结果表明,吴茱萸精油能够显著清除机体外产生的超活性氧,并呈现量效关系.     

间接竞争化学发光酶免疫法检测动物源食品中的 呋喃西林代谢物

目的建立动物源食品中呋喃西林代谢物间接竞争化学发光酶免疫检测方法。方法采用活化酯法将衍生后的呋喃西林代谢物半抗原与卵清蛋白(ovalbumin,OVA)偶联成为包被原;其次,对化学发光液体系、包被原和单克隆抗体的最优稀释倍数及其他反应条件进行优化;最后对该法的灵敏度、特异性、精密度及准确度进行评价。结果化学发光液A液为8 mmol/L对碘苯酚溶液和10 mmol/L鲁米诺溶液(1:1,V:V)混合,B液为每10 m L三(羟甲基)氨基甲烷(Tris)-盐酸缓冲液加5μL 30%H_2O_2溶液,A液与B液临用前体积比1:1混匀;最优反应条件是包被原和单抗稀释倍数均为800,封闭液为1%脱脂乳,竞争时间30 min,酶标二抗孵育60 min;该法的线性方程为Y=-0.4654X+0.3768(r~2=0.993),线性范围为0.123~2.398 ng/m L,IC50为0.544 ng/m L,批内和批间变异系数分别为1.9%~4.1%和2.8%~5.3%,空白鸡肉样品添加回收率为89.6%~98.0%。结论该检测方法简单快速,可用于实验室或现场动物源食品中呋喃西林代谢物的筛查。