In Vivo Albumin-Binding of a C-Functionalized Cyclam Platform for 64Cu-PET/CT Imaging in Breast Cancer Model

An improved glucose-chelator-albumin bioconjugate (GluCAB) derivative, GluCAB-2^Mal, has been synthesized and studied for in vivo ⁶⁴Cu-PET/CT imaging in breast cancer mice models together with its first-generation analogue GluCAB-1^Mal. The radioligand works on the principle of tumor targeting through the enhanced permeability and retention (EPR) effect with a supportive role played by glucose metabolism. [⁶⁴Cu]Cu-GluCAB-2^Mal (99 % RCP) exhibited high serum stability with immediate binding to serum proteins. In vivo experiments for comparison between tumor targeting of [⁶⁴Cu]Cu-GluCAB-2^Mal and previous-generation [⁶⁴Cu]Cu-GluCAB-1^Mal encompassed microPET/CT imaging and biodistribution analysis in an allograft E0771 breast cancer mouse model. Tumor uptake of [⁶⁴Cu]Cu-GluCAB-2^Mal was clearly evident with twice as much accumulation as compared to its predecessor and a tumor/muscle ratio of up to 5 after 24 h. Further comparison indicated a decrease in liver accumulation for [⁶⁴Cu]Cu-Glu-CAB-2^Mal.     

Effect of temperature on the optical and electro-optical properties of poly(methyl methacrylate)/E7 polymer-dispersed liquid crystal composites

Experiments on the effect of temperature on the optical and electro-optical behaviors of polymer-dispersed liquid crystals (PDLCs) are considered. Composite films composed of poly(methyl methacrylate) (PMMA) and the nematic-type liquid crystal (LC) E7 were prepared by solvent casting in chloroform. The PDLC film contained droplets of E7 from 10 to 80 wt % in a PMMA matrix. Morphological studies illustrated the formation of isolated droplets of LC due to phase separation, and their homogeneous distribution increased with increasing E7 content. Thermo-optical studies showed an increase in the nematic–isotropic transition temperature of composites, which indicated preferential solvation during the phase-separation process. The electro-optical characteristics were studied under the conditions of an externally applied square wave electric field with a He–Ne laser (λ = 632.8 nm) as a light source. The responses improved as the E7 content in PMMA increased. Semipermanent memory effects were noticed in composites at higher temperatures. Changes in the transmittance due to thermal variations provided the possibility of using such a device as a temperature sensor. The results obtained indicate that under these experimental conditions, the output can be controlled to the desired level by the selection of a suitable loading of LC to prepare PDLC electro-optically active composite films with a response time on the order of only a few milliseconds. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2008     

Human catalase is imported and assembled in peroxisomes of Saccharomyces cerevisiae

To study the conservation of peroxisomal targeting signals, we have determined the intracellular localization of human peroxisomal catalase when expressed in yeast. Using immunofluorescence, differential centrifugation and immunoelectron microscopy, we show that the protein is targeted to the peroxisomes of the heterologous cell and assembled in its active tetrameric form. These data show the conservation of the catalase targeting signal and import specificity between human and yeast peroxisomes.     

有源电光调制式高功率瞬态电场传感器的研制

瞬态电场防护及应用技术的研究离不开瞬态电场测试系统;文中基于有源电光调制法,研制了一种高功率瞬态电场传感器;通过建立该传感器的等效电路模型,并借助CST仿真建模,分别从频域和时域角度分析了传感器的接收特性,并得到了一致的结果;最后利用脉冲噪声模拟器和吉赫兹横电磁波小室对研制的传感器性能进行了测试;结果表明:接收天线为单极子天线,天线负载采用高阻时响应电压与被测电场场强成正比;研制的传感器带宽为10kHz～314MHz,可用于纳秒级高功率瞬态电场的时域测试。     

两轴稳定平台大俯仰角状态控制技术研究

在对两轴稳定平台一般构型分析的基础上,指出了其在俯仰方向大角度时稳定控制存在的不足,提出了采用三正交陀螺稳定控制方式解决大俯仰角控制问题的方法,最后利用MATLAB进行了数学仿真,结果表明该方法有效提高了系统的稳定精度。     

Multiple Na,K-ATPase Subunits Colocalize in the Brush Border of Mouse Choroid Plexus Epithelial Cells

(1) Background: The unusual accumulation of Na,K-ATPase complexes in the brush border membrane of choroid plexus epithelial cells have intrigued researchers for decades. However, the full range of the expressed Na,K-ATPase subunits and their relation to the microvillus cytoskeleton remains unknown. (2) Methods: RT-PCR analysis, co-immunoprecipitation, native PAGE, mass spectrometry, and differential centrifugation were combined with high-resolution immunofluorescence histochemistry, proximity ligase assays, and stimulated emission depletion (STED) microscopy on mouse choroid plexus cells or tissues in order to resolve these issues. (3) Results: The choroid plexus epithelium expresses Na,K-ATPase subunits α1, α2, β1, β2, β3, and phospholemman. The α1, α2, β1, and β2, subunits are all localized to the brush border membrane, where they appear to form a complex. The ATPase complexes may stabilize in the brush border membrane via anchoring to microvillar actin indirectly through ankyrin-3 or directly via other co-precipitated proteins. Aquaporin 1 (AQP1) may form part of the proposed multi-protein complexes in contrast to another membrane protein, the Na-K-2Cl cotransporter 1 (NKCC1). NKCC1 expression seems necessary for full brush border membrane accumulation of the Na,K-ATPase in the choroid plexus. (4) Conclusion: A multitude of Na,K-ATPase subunits form molecular complexes in the choroid plexus brush border, which may bind to the cytoskeleton by various alternative actin binding proteins.     

Stimuli-Responsive Nanomaterials for Smart Tumor-Specific Phototherapeutics

Phototherapy, a type of photoresponsive regulation of biological activities, together with additional stimuli-responsive features, offers significant potential for enhancing the precision and efficacy of cancer treatments. To achieve tumor-specific therapeutics, numerous studies have focused on the development of smart phototherapeutic nanomaterials (PNMs) that can respond to endogenous pathological characteristics (e. g., mild acidity, the overproduction of glutathione, the overproduction of hydrogen peroxide, the overexpression of specific surface receptors, etc.) present in the tumor and/or exogenous stimuli. Such responsiveness can effectively improve the physicochemical properties, cellular uptake, tumor-targeting performance, and pharmacokinetic profile of PNMs. Herein, we will systematically discuss recent advances in this field. Moreover, potential challenges and future directions in the development of stimuli-responsive PNMs are also presented to support the development of this emerging cutting-edge research area.     

Economic selection of process mean and lot‐sizing decisions for multi‐newsvendor supply chain

Two related problems are integrated in this paper, the first is the targeting problem and another is production/inventory decisions in a supply chain. The supply chain under consideration consists of a supplier of raw material, a single producer, and multiple newsvendors. The producer can adjust the process mean before starting the production run. Once set to a certain target value, the process mean is not changed until the production lot is completed. At the end of a production run, the producer screens the lot and uses specification limits to evaluate the quality of the item. Nonconforming items are scrapped with no salvage value; however, conforming items are delivered to newsvendors who are subjected to random demand. If demand on a newsvendor in a season is lower than anticipated, surplus items will be returned to the producer at a certain transportation cost. We first develop a mathematical model that maximizes the expected total profit of the supply chain. Then, a table for two special functions is devised to simplify the solution method and is used to find the optimal solution of the proposed model. We also study the significance of this integration by comparing the performance of the proposed model with an independent model where the process mean selection and lot‐sizing decisions are found separately.