Photoregulation of Gene Expression with Ligand-Modified Caged siRNAs through Host/Guest Interaction

Small interfering RNA (siRNA) can effectively silence target genes through Argonate 2 (Ago2)-induced RNA interference (RNAi). It is very important to control siRNA activity in both spatial and temporal modes. Among different masking strategies, photocaging can be used to regulate gene expression through light irradiation with spatiotemporal and dose-dependent resolution. Many different caging strategies and caging groups have been reported for light-activated siRNA gene silencing. Herein, we describe a novel caging strategy that increases the blocking effect of RISC complex formation/process through host/guest (including ligand/receptor) interactions, thereby enhancing the inhibition of caged siRNA activity until light activation. This strategy can be used as a general approach to design caged siRNAs for the photomodulation of gene silencing of exogenous and endogenous genes.     

“任务驱动”教学法在可编程控制器课程教学中的实践与分析

以可编程控制器课程为例,介绍"任务驱动"教学法在教学过程中的具体应用,并探讨了实施过程中存在的问题与解决方法。     

Identification of Host Cellular Protein Substrates of SARS-COV-2 Main Protease

The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease-19 (COVID-19) being associated with severe pneumonia. Like with other viruses, the interaction of SARS-CoV-2 with host cell proteins is necessary for successful replication, and cleavage of cellular targets by the viral protease also may contribute to the pathogenesis, but knowledge about the human proteins that are processed by the main protease (3CLpro) of SARS-CoV-2 is still limited. We tested the prediction potentials of two different in silico methods for the identification of SARS-CoV-2 3CLpro cleavage sites in human proteins. Short stretches of homologous host-pathogen protein sequences (SSHHPS) that are present in SARS-CoV-2 polyprotein and human proteins were identified using BLAST analysis, and the NetCorona 1.0 webserver was used to successfully predict cleavage sites, although this method was primarily developed for SARS-CoV. Human C-terminal-binding protein 1 (CTBP1) was found to be cleaved in vitro by SARS-CoV-2 3CLpro, the existence of the cleavage site was proved experimentally by using a His₆-MBP-mEYFP recombinant substrate containing the predicted target sequence. Our results highlight both potentials and limitations of the tested algorithms. The identification of candidate host substrates of 3CLpro may help better develop an understanding of the molecular mechanisms behind the replication and pathogenesis of SARS-CoV-2.     

基于PLC的煤矿排水监控系统研究

该文建立了由数据采集、主排水控制以及地面监控三个部分组成的煤矿主排水系统。对以西门子S7-300 PLC作为中央处理器的硬件系统进行了简单分析,并绘制了软件流程图。该系统的运行充分考虑了井下排水要求和节能要求,根据供电的不同时段进行水泵的不同控制。因此,该系统对于煤矿的安全生产和绿色生产具有一定的实践意义。     

CN decoration of dibenzofuran modified biphenyl for high triplet energy host for blue phosphorescent organic light-emitting diodes

A strongly electron deficient and high triplet energy host for blue emitters was developed by decorating a dibenzofuran modified biphenyl backbone structure with multiple CN units. Two hosts, 6,6′-bis(6-cyanodibenzo[b,d]furan-4-yl)-[1,1′-biphenyl]-3,3′-dicarbonitrile(CNDBF1) and 2,2′-bis(6-cyanodibenzo[b,d]furan-4-yl)-[1,1′-biphenyl]-4,4′-dicarbonitrile(CNDBF2), were derived from the CN decoration strategy for application in blue organic light-emitting diodes requiring high triplet energy host. They showed high triplet energy above 2.79 eV and acted as the electron transport type host based on the strong electron deficiency. The mixture of the CNDBF1 and CNDBF2 hosts with a hole transport type 3,3′-di(9H-carbazol-9-yl)-1,1′-biphenyl host performed as the exciplex host of a blue phosphor and accomplished high external quantum efficiency of 22.7% in the blue phosphorescent organic light-emitting diodes.     

机电制造企业生产系统的仿真

针对我国机电企业的现状，研究并完成了机电制造企业的物流监控信息系统的典型设计，建立了质量跟踪体系软件结构，进行计算机仿真，并应用于现场。研究涉及计算机控制技术、信息处理技术、工业以太网通讯技术、现场总线通讯技术、最优化策略等多学科技术。该系统可以大幅度提高生产效率、降低生产成本、减少库存和资金占用，减少日益昂贵的生产和库存用地。     

Adsp-21060的主机接口在实时图像处理中的应用

简要介绍了数字信号处理器Adsp 2 10 6 0和大规模可编程逻辑器件EP1K5 0。详细讨论了Adsp 2 10 6 0的主机接口工作模式以及一个实时图像跟踪处理器的硬件组成原理 ,在这个系统中EP1K5 0充当了主机。调试结果表明 ,所提出的硬件结构设计思想工作效率高 ,完全能够胜任实时图像处理的实际需要。     

工业稳定剂间歇过程计算机控制系统

介绍了1套由AB公司的第5代PLC——RSLogix5000和工业PC组成的工业稳定剂间歇过程计算机控制系统。较详细地介绍系统硬件、软件的构成，ControlNet现场总线网络通信的特点，OPC数据传输的优点。同时阐述了在系统硬件设计上如何保证硬件的安全性和抗干扰问题。运行结果表明。该控制系统具有维护简单、运行可靠性高、抗干扰能力强、传输数据速度快的特点。     

PLC在广播发射台的应用

本文主要介绍了PLC即可编程序控制器的工作原理和编程语言,重点介绍了PLC在发射台的天线交换系统以及发射机控制系统中的应用。     

几种涉密网主机系统保护及审计技术的比较

论文比较详细地阐述了涉密网主机系统所面临的安全隐患,提出了涉密网主机安全防护所要解决的几个技术问题,并就可能实现的技术方法进行了探讨和比较。