Development and monitoring of an integrated bioprocess for production of a potential malaria vaccine with Pichia pastoris

An engineered variant of the yeast Pichia pastoris was used to produce the potential malaria vaccine D1M1. This paper describes the implementation of an expanded bed adsorption chromatography step into a sequential cultivation process of the yeast in order to link protein expression, cell release, and product capture of the secreted protein in a fully automated plant. The process is operated with a semi-continuous strategy for an integrated production. Process improvements have been achieved using design of experiments. Resulting purities of product achieved up to 87% with recoveries of 51% in a single downstream operation. Furthermore, a multivariate analysis of historical batch data was used to develop a golden batch model for process monitoring.     

谈皎然文论中儒家中庸之道与佛家中道思想的沟通

中唐诗僧皎然的诗歌理论著作《诗式》立足于儒学,由儒而入佛,并试图通过儒学来沟通佛学。其诗学理论体现了儒家中庸之道与佛家中道思想的融合与沟通。     

论泸州在中国白酒金三角区域旅游中的定位与融入

涵盖泸州、宜宾、遵义三地市的中国白酒金三角区域旅游正在逐步形成,泸州市如何在此区域旅游中找准定位,形成和凸显自身特色,从而融入白酒金三角区域经济发展,本文在调研基础上提出了对策建议。     

黄金分割法结合UTD计算机载天线方向图

机载天线辐射特性的研究,对机载天线的一体化设计、布局以及机载电子系统的电磁兼容性(EMC)具有非常重要的意义.应用一致性几何绕射理论(UTD)分析机载天线的辐射特性,利用黄金分割法(0.618法)优化二阶射线寻迹,并考虑了遮挡的判断问题,提高了运算的速度和精度.以机载单极子天线为例.给出了机载天线远场方向图的仿真结果.结果比较令人满意,证明了方法是正确的,并对机载天线耦合分析有一定参考意义.     

特征模型方法在中继星天线指向控制中的应用

主要研究基于特征模型的自适应控制方法在中继星天线指向跟踪控制中的应用.利用由Newton-Euler方法建立的挠性多体卫星的混合坐标方程动力学简化模型进行数学仿真,分别采用基于特征模型的自适应控制方法和经典PID方法进行卫星本体姿态和天线指向跟踪控制,并比较两种控制方法的控制稳定性和控制精度,仿真结果表明用特征模型自适应控制方法设计的控制器能满足系统性能要求,并具有很强的鲁棒性和抗干扰性.     

金鹰奥莱城的SWOT分析及战略选择

战略规划对企业具有举足轻重的地位,恰当的战略可以为企业的发展指明方向。SWOT分析法综合考虑企业内外部的因素,为企业选择最佳的经营战略。本文采用SWOT分析法对金鹰奥莱城进行深入细致的研究,通过分析影响该企业的内外部因素,探索金鹰奥莱城的战略选择。     

Building a Golden Grove ‘community’: a study of suburban production processes in South Australia, 1970s–1980s

Ideals of community have been associated with the practice of planning for over a century. Proponents of the Golden Grove Development considered particular ideals of community to be fundamental to the financial and social success of the new suburban development. This paper will analyse the early planning processes that underpinned the production of community ideals and argue that these planning processes took place at the transition between welfarism and neo‐liberalism. This study will offer provocative glimpses into late twentieth century suburban planning practices; practices that establish the Golden Grove Development as a model of late twentieth century suburban production processes.     

低温快烧金光釉的研制

作者以正交试验法研究探讨了多种金属氧化物对金光釉产生金属光泽及釉面质量的影响，采用快速烧成的工艺探索了金光釉快速烧成的可行性，并在氧化还原不同的气氛下验证了金光釉对气氛的适应性，从理论上分析了金光釉产生金属光泽的机理。     

Length to diameter ratio of extrudates in catalyst technology I. Modeling catalyst breakage by impulsive forces

Natural or forced catalyst extrudate breakage is an important phenomenon during catalyst manufacture. Here, a two‐parameter model for predicting the reduction in the length to diameter ratio of catalyst extrudates due to breakage by impulsive forces as experienced in a laboratory drop test is developed. Part II will show how both parameters can be correlated with the strength of the extrudates and the severity of the drop test. For breakage by impulsive forces, the model reveals that extrudates are reduced in length to diameter ratio according to a pseudosecond‐order break law. Also, a tie‐in exists with the well‐known Golden Ratio that is famous for its inherent esthetic value. Applying the model to cases of “severity sequencing” and “severity conditioning” reveals the nonlinear behavior of the length to diameter ratio and yields results that are often nonintuitive and hard to get without this engineering analysis. © 2015 American Institute of Chemical Engineers AIChE J, 62: 639–647, 2016     

金帅苹果多酚氧化酶提取及部分酶学特性研究

实验确定了从金帅苹果中提取多酚氧化酶(PPO)的最佳条件,研究了该酶的某些特性。确定pH7.4磷酸氢二钠-柠檬酸缓冲液(含2%的PVPP和0.25%TritonX100)、料液比1.6mL/g为最优提取参数。金帅苹果PPO酶促反应产物在412nm下有最大吸收峰;以邻苯二酚为底物的酶促反应的米氏常数为Km=2.6667×10-2mol/L,PPO动力学方程为:V=3333.33犤S犦0.027 犤S犦;该PPO的最适pH为5.0,在pH5.0～6.8范围内有较高的稳定性;最适温度为40℃。