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1.
Dr. Emrah Kara Dr. Nis Valentin Nielsen Bergrun Eggertsdottir Dr. Bernd Thiede Dr. Sandip M. Kanse Dr. Geir Åge Løset 《Chembiochem : a European journal of chemical biology》2020,21(13):1875-1884
We describe a novel, easy and efficient combinatorial phage display peptide substrate-mining method to map the substrate specificity of proteases. The peptide library is displayed on the pVII capsid of the M13 bacteriophage, which renders pIII necessary for infectivity and efficient retrieval, in an unmodified state. As capture module, the 3XFLAG was chosen due to its very high binding efficiency to anti-FLAG mAbs and its independency of any post-translational modification. This library was tested with Factor-VII activating protease (WT-FSAP) and its single-nucleotide polymorphism variant Marburg-I (MI)-FSAP. The WT-FSAP results confirmed the previously reported Arg/Lys centered FSAP cleavage site consensus as dominant, as well as reinforcing MI-FSAP as a loss-of-function mutant. Surprisingly, rare substrate clones devoid of basic amino acids were also identified. Indeed one of these peptides was cleaved as free peptide, thus suggesting a broader range of WT-FSAP substrates than previously anticipated. 相似文献
2.
本文对芝麻营养保健成分的提取进行了系统的研究。确定了芝麻营养原液的制取工艺。为充分发挥芝麻的营养保健作用开辟了新的途径。 相似文献
3.
To gain a better understanding of the ultra-high molecular weight polyethylene (UHMWPE) wear mechanism in the physiological environment, the effects of protein and lipid constituents of synovial fluid on the specific wear rate of UHMWPE were examined experimentally. The multidirectional sliding pin-on-plate wear tester was employed to simulate the simplified sliding condition of hip joint prostheses. Bovine serum γ-globulin and synthetic l--DPPC were used as model protein and lipid constituents of synovia, respectively. Results of the wear test indicated that the UHMWPE wear rate primarily depended on the protein concentration of the test lubricant. Lipids acted as a boundary lubricant and reduced polyethylene wear in the low protein lubricants. However, the polyethylene wear rate increased with increasing lipid concentrations if the protein concentration was within the physiological level. Increased interactions between protein and lipid molecules and lipid diffusion to polyethylene surface might be responsible for the increased wear. 相似文献
4.
Suzana Corte Real Maria de Nazareth Leal de Meirelles 《Microscopy research and technique》1991,18(2):192-196
Immunocytochemistry was used to investigate the localization of F1 ATPase in mitochondria of cryosections of adult mouse heart muscle cells. The initial aldehyde fixation was the only denaturation step for antigens. The fine structure was preserved with contrast enhancement as the sections were maintained hydrated, with the advantage that the entire procedure is completed in one working day. The reaction was highly specific, and entire mitochondria were labeled with the Protein A-gold complex. A new analytical technique, electron spectroscopic imaging (ESI), contributed to a better visualization of the localization of the F1 factor. 相似文献
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本文列出影响用凯氏定氮仪测定蛋白质结果的因子,依据测量不确定度评定规范,尝试对测定蛋白质结果不确定度的评价。 相似文献
6.
国际乳业的热点论题:蛋白质标准化 总被引:1,自引:0,他引:1
介绍了蛋白质的标准化这一国际乳品界正热的研究和讨论题目,综述了蛋白质标准化的必要性和世界上目前采用的方法,展望了膜技术在蛋白质标准化上的应用前景。 相似文献
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淋病奈瑟菌外膜蛋白Ⅰ的分离提纯及鉴定 总被引:2,自引:0,他引:2
在CaCl2存在下,以CTB提取淋球菌的OMP,再以Z3,14萃取PI,然后经DEAESepharoseCl-6B层析柱提纯,获得纯PI10.97毫克。SDS-PAGE显示纯PI为单一蛋白区带,分子量为35KD,经可见光扫描,其纯度达96.21%。用ELISA间接法检测证明,纯PI保持完好的抗原活性。亚类特异性鉴定证实为WI群PIA。 相似文献
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