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1.
《Food Control》2015
A diverse range of genetic elements has been used to develop genetically modified organisms (GMOs) over the last 18 years. Screening methods that target few elements, such as the Cauliflower Mosaic Virus 35S promoter (P-35S) and Agrobacterium tumefaciens nopaline terminator (T-nos), are not sufficient to screen GMOs. In the present study, a multiplex PCR system for all globally commercialized GM soybean events was developed to easily trace the events. For this purpose, screening elements of 24 GM soybean events were investigated and 9 screening targets were selected and divided into three individual triplex PCR systems: P-35S, ribulose-1,5-bisphosphate carboxylase small subunit promoter of Arabidopsis thaliana, T-nos, T-35S, pea E9 terminator, open reading frame 23 terminator of A. tumefaciens, proteinase inhibitor II terminator of potato, acetohydroxy acid synthase large subunit terminator of A. thaliana, and the revealed 3′ flanking sequences of DP-305423-1. The specificity of the assays was confirmed using thirteen GM soybean events as the respective positive/negative controls. The limit of detection of each multiplex set, as determined using certified reference materials of specific GM events, ranged from 0.03 to 0.5%, depending upon target. Furthermore, 26 food samples that contained soybean ingredients, which were purchased from the USA, China, Japan, and Korea, were analyzed, 17 of which contained one or more GM soybean events. These results suggest that the developed screening method can be used to efficiently track and identify 24 GM soybean events in food and feed. 相似文献
2.
Considering autonomous mobile robots with a variety of specific functions as a kind of service, when there are many types and quantities of services and the density of regional services is large, proposing an algorithm of Circular Area Search (CAS) because of the problem of multi-robot service scheduling in various areas. Firstly, Django is used as the web framework to build the Service-Oriented Architecture (SOA) multi-robot service cloud platform, which is the basic platform for multi-service combination. Then, the service type, the latitude and longitude and the scoring parameters of the service are selected as the service search metrics to design the CAS algorithm that based on the existing service information registered in MySQL and the Gaode Map for screening optimal service, and then providing the service applicant with the best service. Finally, the service applicant applies for the self-driving tour service as an example to perform performance simulation test on the proposed CAS algorithm. The results show that the CAS algorithm of the multi-robot service cloud platform proposed in this paper is practical compared to the global search. And compared with the Greedy Algorithm experiment, the service search time is reduced about 58% compared with the Greedy Algorithm, which verifies the efficiency of CAS algorithm. 相似文献
3.
Sung D. Kwon Sung J. Song Dong H. Bae Young Z. Lee 《Journal of Mechanical Science and Technology》2002,16(9):1084-1092
The frequency dependency of Rayleigh surface wave is investigated indirectly by measuring the angular dependency of the backward
radiation of the incident ultrasonic wave in two kinds of degraded specimens by scuffing or corrosion. Then, the frequency
dependency is compared with the residual stress distribution or the corrosion-fatigue characteristics for the scuffed or corroded
specimens, respectively. The width of the backward radiation profile increases with the increase of the variation in residual
stress distribution for the scuffed specimens. In the corroded specimens, the profile width decreases with the increase of
the effective aging layer thickness and is inversely proportional to the exponent, m, in the Paris’ law that can predict the
crack size increase due to fatigue. The result observed in this study demonstrates high potential of backward radiated ultrasound
as a tool for nondestructive evaluation of subsurface gradient of material degradation generated by scuffing or corrosion. 相似文献
4.
M. D. Beard M. J. S. Lowe 《International Journal of Rock Mechanics and Mining Sciences》2003,40(4):527-536
The research outlined in this paper is aimed at the development of a portable non-destructive testing instrument for evaluating the condition of rock bolts. In applications such as coal mine roof reinforcement, the opportunities for rock bolt inspection are currently limited to destructive techniques such as the pull-out test. It is proposed that guided ultrasonic waves can be used to solve this inspection problem, using a pulse-echo test carried out from the free end of the bolt. Suitable test frequencies have been identified through the use of modelling software, and successful laboratory and site trials have been undertaken. The research has shown that the proposed approach is capable of determining the bolt length, and of identifying major defects such as necking, deformation, and loss of resin encapsulation. 相似文献
5.
Ultrasound backscattered signals for object detection could be too weak to be perceived when superposed to strong reflection signals, and could also be complicated either in time or frequency domain. These peculiarities raise a challenge for signal processing methods. In this paper four signal processing methods were studied to detect objects in containers. Root mean squares (RMS) method was better in object detection but needed to combine other methods to increase detection success rate. Variance, center frequency pressure ratio (CFPR) and backscattered amplitude integral (BAI) methods have similar overall detection ratings. The optimal signal size, to increase the detection ability, corresponded approximately to the length of reverberation signals before it was dissipated. 相似文献
6.
7.
富集孢粉化石的一种方法 总被引:2,自引:0,他引:2
本论述了孢粉,沟鞭藻化石与矿物颗粒相互分离的方法,并与传统的氢氟酸冷处理方法进行实验对比。结果表明,该方法摒弃有毒重液,提高了化石收获率。 相似文献
8.
介绍了一种新型悬臂棒条筛分机械,论述了其结构、工作原理和技术特性。从理论和实践两方面论证了它的结构、处理能力、筛分效率、返矿率都明显优于普通悬臂棒条筛分机械,可广泛应用于高炉槽下烧结矿的筛分作业。 相似文献
9.
针对辽河油田高凝油区块注高温热水(85—95℃)引起的腐蚀、结垢,开展了室内和现场缓蚀、阻垢试验;分析了不同温度下注水的腐蚀情况,对比、考察了不同的缓蚀剂、阻垢剂及其复配物,得出了如下认识:在注高温热水条件下直接采用常温下使用的防腐蚀、防垢方法是不可行的,必须有针对性地进行高温条件下防腐蚀、防垢的实验研究工作。 相似文献
10.
Performance of the polymer- and oxide-supported triphase catalysts and effect of ultrasound on their stabilities 总被引:1,自引:0,他引:1
Hsieh-Ting Chung Hung-Shan Weng 《Journal of the Chinese Institute of Chemical Engineers》2008,39(5):449-455
In this study, several trialkylamines were immobilized on chloromethylated polystyrene (CMPS), silica gel, and alumina to prepare triphase catalysts for catalyzing the etherification reaction of allyl bromide (the organic reactant) and sodium phenolate (the aqueous reactant). The reactor was agitated mechanically or with the aid of ultrasonic vibration. Performances of the prepared catalysts were compared, and the effect of imposing ultrasound was investigated based on the activity, selectivity, and stability of the catalyst. Experimental results show that tri-n-propylamine is the best active species when CMPS is used as the support, while tri-n-butylamine is the best when SiO2 and Al2O3 are employed as the supports. The CMPS-supported catalyst is far better than the SiO2- and Al2O3-supported catalysts in activity and selectivity but not in stability. Imposing the ultrasound can effectively increase the reaction rate. Mechanical agitation at a low speed with the imposition of ultrasonic vibration not only results in a conversion slightly higher than the case with a high mechanical agitation speed without ultrasonic vibration, but also gives a constant stability for the CMPS-supported catalyst. 相似文献