Novel approaches to purifying bacteriocin: A review

ABSTRACT

Bacteriocin is a proteinaceous biomolecule produced by bacteria (both Gram-positive and Gram-negative) that exhibits antimicrobial activity against closely related species, and food-borne pathogens. It has recently gained importance and attracted the attention of several researchers looking to produce it from various substrates and bacterial strains. This ushers in a new era of food preservation where the use of bacteriocin in food products will be an alternative to chemical preservatives, and heat treatment which are understood to cause unwanted side effects, and reduce sensory and nutritional quality. However, this new market depends on the success of novel downstream separation schemes from various types of crude feedstocks which are both effective and economic. This review focuses on the downstream separation of bacteriocin from various sources using both conventional and novel techniques. Finally, recommendations for future interesting areas of research that need to be pursued are highlighted.     

Liner services network design and fleet deployment with empty container repositioning

Transportation demand of shipping container fluctuates due to the seasonality of international trade, thus, every 3–6 months, the liner company has to alter its current liner shipping service network, redeploy ships and design cargo routes with the objective of minimizing the total cost. To solve the problem, the paper presents a mixed integer linear program model. The proposed model incorporates several relevant constraints, such as weekly frequency, the transshipment of cargo between two or more service routes, and transport time. Extensive numerical experiments based on realistic date of Asia–Europe–Oceania shipping operations show that the proposed model can solve real-case problems efficiently by CPLEX. The results demonstrate that the model can reduce ship’s capacity consumption and raise ships’ capacity utilization.     

嵌入式实时操作系统内核混合代码的自动化验证框架

"如何构造高可信的软件系统"已成为学术界和工业界的研究热点.操作系统内核作为软件系统的基础组件，它的安全可靠是构造高可信软件系统的重要环节.为了确保操作系统内核的安全可靠，将形式化方法引入到操作系统内核验证中，提出了一个自动化验证操作系统内核的框架.该验证框架包括：（1）分别对C语言程序和混合语言程序（C语言和汇编语言）进行验证；（2）在混合语言程序验证中，为汇编程序建立抽象模型，并将C语言程序和抽象模型粘合形成基于C语言验证工具可接收的验证模型；（3）从规范中提取性质，基于该自动验证工具，对性质完成自动验证；（4）该框架不限于特定的硬件架构.成功地运用该验证框架对两种不同硬件平台的嵌入式实时操作系统内核μC/OS-II进行了验证.结果显示：利用该框架在对两个不同的硬件平台上内核验证时，框架的可重复利用率很高，高达到88%，虽然其抽象模型需要根据不同的硬件平台进行重构.在对基于这两种平台的操作系统内核验证中，分别发现了10~12处缺陷.其中，在ARM平台上两处与硬件相关的问题被发现.实验表明，该方法对不同硬件平台的同一个操作系统分析验证具有一定的通用性.     

针对不同地质目标的叠前时间偏移成像解释评价

随着计算机硬件和地震勘探成像技术的发展，叠前时间偏移正逐步替代常规的NMO加DMO加叠后时间偏移成为地震数据成像处理方法的主流。但对于不同的地质目标，叠前时间偏移的成像效果是否优于常规NMO加DMO加叠后时间偏移的成像效果呢？为此，从地震数据成像处理方法、处理流程和处理参数等方面进行了讨论，并基于某地区三维数据常规处理结果和叠前时间偏移处理结果，针对不同地质目标进行了剖析与评价。认为：叠前时间偏移成像的垂向分辨率较常规处理明显降低，但对于空间波阻抗变化明显的河流和断层，叠前时间偏移成像的空间分辨率要高于常规处理；对于小于1／4波长的叠置薄储层，叠前时间偏移成像的垂向和空间分辨率低于常规处理结果。     

普通稠油油藏启动压力梯度求解方法与应用

利用微观尺度管流模型,结合普通稠油流变特征,提出了在考虑稠油流变性条件下微观启动压力梯度计算方法.对渤海渤中18块不同渗透率岩心进行室内驱替实验,通过对实验数据的回归分析,得到不同粘度普通稠油油藏启动压力梯度与地层平均渗透率关系,并绘制了启动压力梯度的图版.将研究结果应用于普通稠油油藏,给出了利用启动压力梯度确定极限泄油半径及合理井距的理论计算方法.     

浅谈如何有效学习计算机软件知识

计算机软件知识的学习并不是完全线性的，而是不断循环往复、迭代的过程。本对如何深入、广泛、有效地学习计算机软件知识做出了一些探讨。     

西部地区深井井身结构设计技术探讨

科学合理地确定井身结构及钻井液密度是深井超深井钻井的关键环节之一，其基础是准确建立地层孔隙压力、地应力、破裂压力、坍塌压力的钻前预测剖面。分析了西部地区目前深井井身结构的缺点和进行井身结构应考虑的问题，介绍了深探井井身结构的设计方法，并针对西部复杂地区的地层特点，建议采用3种增加技术套管的井身结构方案。塔河油田钻井实践表明，采用这3种井身结构方案后，深井机械钻速得到提高，钻井周期缩短，而且降低了钻井成本。     

A three-dimensional FE analysis of large deformations for impact loadings using tetrahedral elements

 A three-dimensional dynamic program for the anaysis of large deformations in contact-penetration problems is developed using the finite element Lagrangian method with explicit time integration. By incorporating a tetrahedral element, which allows a single-point integration without a special hourglass control scheme, this program can be more effective to the present problem. The position code algorithm is used to search contact surface. Eroding surfaces are also considered. The defense node algorithm was slightly modified for the calculation of contact forces. A study of obliquity effects on metallic plate perforation and ricochet processes in thin plates impacted by a sphere was conducted. It is well simulated that on separation of two parts of the sphere, the portion still within the crater tends to perforate, while the portion in contact with the plate surface ricochets. This deformation pattern is observed in experiments, especially at high obliquities. A long rod that impacts an oblique steel plate at high impact velocity was also simulated in order to study the dynamics of the rod caused by the three dimensional asymmetric contact. The agreement between simulated and experimental results is quite good. Fracture phenomena occuring at high obliquity deserves further investigations. Received: 20 February 2002 / Accepted: 20 September 2002     

车古201潜山油藏完井方式研究

研究了国内外潜山油藏完井方式应用及其使用条件、井身结构的应用 ,针对车古 2 0 1油藏特点 ,论证了其对裸眼完井、筛管完井、尾管射孔完井、管外封隔器完井的适应性 ,通过对比研究 ,从更好地满足油藏工程、采油工艺、增产措施出发 ,选择出车古 2 0 1潜山油藏最合理的完井方式为尾管注水泥射孔完井 ,并给出了应采用的井身结构     

程序可视化系统ProVS的设计和实现

本文研究程序代码可视化技术，即从现存的程序生成某种可视化表示，增强程序的易读性、易理解性和易维护性。首先介绍ＰｒｏＶＳ的设计思想和实现技术，然后给出输出例子。