In Vivo Albumin-Binding of a C-Functionalized Cyclam Platform for 64Cu-PET/CT Imaging in Breast Cancer Model

An improved glucose-chelator-albumin bioconjugate (GluCAB) derivative, GluCAB-2^Mal, has been synthesized and studied for in vivo ⁶⁴Cu-PET/CT imaging in breast cancer mice models together with its first-generation analogue GluCAB-1^Mal. The radioligand works on the principle of tumor targeting through the enhanced permeability and retention (EPR) effect with a supportive role played by glucose metabolism. [⁶⁴Cu]Cu-GluCAB-2^Mal (99 % RCP) exhibited high serum stability with immediate binding to serum proteins. In vivo experiments for comparison between tumor targeting of [⁶⁴Cu]Cu-GluCAB-2^Mal and previous-generation [⁶⁴Cu]Cu-GluCAB-1^Mal encompassed microPET/CT imaging and biodistribution analysis in an allograft E0771 breast cancer mouse model. Tumor uptake of [⁶⁴Cu]Cu-GluCAB-2^Mal was clearly evident with twice as much accumulation as compared to its predecessor and a tumor/muscle ratio of up to 5 after 24 h. Further comparison indicated a decrease in liver accumulation for [⁶⁴Cu]Cu-Glu-CAB-2^Mal.     

AT91SAM7S64芯片I2C(TWI)接口应用开发

较为详细地介绍AT91SAM7S64芯片的I2C接口控制器中外围数据控制器(PDC)的使用方式,并将其与AT24C04之间的通信作为实例给出完整的应用开发流程和软硬件详细描述。该方法的可行性已经为工程实践和实际产品所证实。该操作也可推广到其他AT91SAM7S系列ARM处理器与其他I2C接口器件间的通信。并对其他拥有外围数据控制器的接口开发有一定的借鉴意义。     

Copper bioavailability from breakfasts containing tea. Influence of the addition of milk

The influence of drinking tea on copper bioavailability is unclear, particularly when tea is consumed with food. A breakfast meal containing white bread, margarine, strawberry jam, cheese and tea, with or without milk, was digested in; vitro and the dialysis of copper investigated. Reference breakfasts were prepared with water and water with milk. Copper dialysability (percentage of copper dialysed) was markedly increased by tea. The influence of adding milk to tea was not significant. A second study was carried out in rats using the breakfasts containing tea and water. They were spiked with ⁶⁴Cu and given orally to the animals in a single dose. Whole-body ⁶⁴Cu retention was measured over a 4-day period after administration. In order to determine the true absorption of ⁶⁴Cu a control group of rats received the dose intraperitioneally. The results in vivo indicate a tendency to higher absorption and retention of ⁶⁴Cu with tea. Liver ⁶⁴Cu retention was significantly elevated by tea. Therefore, the presence of tea in a breakfast meal favours the formation of soluble low-molecular-weight ligands which can be absorbed and retained by rats. The concomitant increase in liver copper retetition may be associated with higher bioavailability and/or lower copper mobilisation.     

Tru64 Unix服务器中系统及进程信息的获取方法

介绍了通过访问/proc伪文件系统,获得Tru64操作系统系统的进程信息的方法,以及根据所取得的数据计算进程CPU占用率和内存等信息的算法。还介绍了通过Tru64系统特有的API函数获得系统信息的方法。并对基于此方法取得的试验数据与top、SystemInformation、ProcessTuner的数据作比较。     

基于C#语言的图片插入方法比较

图片是传递与表达信息的重要形式,由于图片数据特殊编码方式,它的存储和显示显得比较复杂。C#功能强大,使用方便,已经成为一种主流的计算机软件编程语言。文章介绍了使用C#向数据库中插入图片的方法,并加以比较。     

Real-Time Monitoring the Effect of Cytopathic Hypoxia on Retinal Pigment Epithelial Barrier Functionality Using Electric Cell-Substrate Impedance Sensing (ECIS) Biosensor Technology

Disruption of retinal pigment epithelial (RPE barrier integrity is a hallmark feature of various retinal blinding diseases, including diabetic macular edema and age-related macular degeneration, but the underlying causes and pathophysiology are not completely well-defined. One of the most conserved phenomena in biology is the progressive decline in mitochondrial function with aging leading to cytopathic hypoxia, where cells are unable to use oxygen for energy production. Therefore, this study aimed to thoroughly investigate the role of cytopathic hypoxia in compromising the barrier functionality of RPE cells. We used Electric Cell-Substrate Impedance Sensing (ECIS) system to monitor precisely in real time the barrier integrity of RPE cell line (ARPE-19) after treatment with various concentrations of cytopathic hypoxia-inducing agent, Cobalt(II) chloride (CoCl₂). We further investigated how the resistance across ARPE-19 cells changes across three separate parameters: R_b (the electrical resistance between ARPE-19 cells), α (the resistance between the ARPE-19 and its substrate), and C_m (the capacitance of the ARPE-19 cell membrane). The viability of the ARPE-19 cells and mitochondrial bioenergetics were quantified with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and seahorse technology, respectively. ECIS measurement showed that CoCl₂ reduced the total impedance of ARPE-19 cells in a dose dependent manner across all tested frequencies. Specifically, the ECIS program’s modelling demonstrated that CoCl₂ affected R_b as it begins to drastically decrease earlier than α or C_m, although ARPE-19 cells’ viability was not compromised. Using seahorse technology, all three concentrations of CoCl₂ significantly impaired basal, maximal, and ATP-linked respirations of ARPE-19 cells but did not affect proton leak and non-mitochondrial bioenergetic. Concordantly, the expression of a major paracellular tight junction protein (ZO-1) was reduced significantly with CoCl_2-treatment in a dose-dependent manner. Our data demonstrate that the ARPE-19 cells have distinct dielectric properties in response to cytopathic hypoxia in which disruption of barrier integrity between ARPE-19 cells precedes any changes in cells’ viability, cell-substrate contacts, and cell membrane permeability. Such differences can be used in screening of selective agents that improve the assembly of RPE tight junction without compromising other RPE barrier parameters.     

基于采样尺度自适应的多尺度量子谐振子优化算法并行化

多尺度量子谐振子优化算法MQHOA是基于量子波函数理论提出的元启发式算法,传统MQHOA寻优过程中不同个体的采样尺度不具有差异性,这种机制限制了解的多样性。针对适应度不同的采样个体,提出采样尺度自适应策略,将采样情况差的个体采样尺度合理扩大,增加迭代过程中不同采样个体所使用采样尺度的多样性,并基于采样尺度的差异性提出并行化框架。选取7组测试函数将改进后的算法（MQHOA-PS）与MQHOA在华为鲲鹏920和AMD EPYC 7452处理器上进行测试实验,实验结果表明,改进后的算法寻优具有较高的精度和成功率,并且所需时间更短。     

ARM计算环境下堆芯程序的移植

为了论证国产芯片在堆芯数值计算领域的可行性,对多个堆芯程序在飞腾处理器的ARM通用计算环境中进行了移植,涉及堆芯燃料管理软件的扩散原型程序NACK-R、子通道分析程序CORTH、特征线输运程序OpenMOC和堆芯组件程序KYLIN2。移植过程在ARM计算环境中通过合理的程序代码修订,去除对商业函数库的依赖,且在移植过程中对KYLIN2的特征线循环扫描计算过程引入OpenMP多线程并行,论证单结点多个飞腾处理器核心的并行能力。参照对象Intel商用处理器的频率约为飞腾处理器频率的2倍,堆芯程序移植后的串行运行效率与在Intel计算环境中的串行运行效率差异保持在3~4倍,受限于所使用飞腾处理器型号的缓存大小,部分数据量较大例题的性能差异可能更大。KYLIN2完成多线程并行后计算效率接近在Intel处理器上的串行效率,证明单结点多个飞腾处理器核心能够替换部分堆芯数值计算既有的应用场景。移植结果也表明,混合不同处理器的异构设计,能够在计算资源紧张的情况下充分利用国产硬件,提升计算环境的整体利用效率。