排序方式: 共有21条查询结果,搜索用时 31 毫秒
1.
Hirofumi Hirayama Toshihiro Itoh Yoshihiko Nakaki Takahiko Yoshida 《Artificial Life and Robotics》2005,9(3):144-160
A mathematical method is introduced to characterize the electrokinetic behavior (electrophoresis) of a biomolecular particle which passes through a specific channel pore on an excitable biological membrane. The basic approach was first proposed by Booth (1950). The system was described by an equation of continuity and an equation of motion in which the driving force involves the diffusion effect, the hydrostatic pressure, and the electrostatic potential. By assuming linear relations between the velocity and the applied electrical field, solutions for the potential, pressure, and velocity were given by a series expansion of the charges on the particle. To examine the influence of ions surrounding the particle and forming an ionic cloud, the Debye–Huckel parameter was introduced. As the thickness of the double layer around the particle increased, the potential, velocity, pressure, and viscosity were changed significantly. The maximum influence was obtained when the radius of the particle became equal to the thickness of the double layer. Although this theory is valid for a charged, spherical, nonconducting particle only, the method is available for evaluating the kinetic behavior of a biomolecule that passes through a channel pore on a cellular membrane.This work was presented, in part, at the 8th International Symposium on Artificial Life and Robotics, Oita, Japan, January 24–26, 2003 相似文献
2.
周凌云 《昆明理工大学学报(自然科学版)》1995,(2)
藉助分形分维理论和热传导理论,提出了生物分子的解链温度及激光辐照生物体而使生物分子解链的时间门槛值。 相似文献
3.
The knowledge of interactions among functional proteins helps researchers understand disease mechanisms
and design potential strategies for treatment. As a general approach, the fluorescent and affinity tags were employed
for exploring this field by labeling the Protein of Interest (POI). However, the autofluorescence and weak binding
strength significantly reduce the accuracy and specificity of these tags. Conversely, HaloTag, a novel self-labeling
enzyme (SLE) tag, could quickly form a covalent bond with its ligand, enabling fast and specific labeling of POI.
These desirable features greatly increase the accuracy and specificity, making the HaloTag a valuable system for
various applications ranging from imaging to immobilization of POI. Notably, the HaloTag technique has already
been successfully employed in a series of studies with excellent efficiency. In this review, we summarize the
development of HaloTag and recent advanced investigations associated with HaloTag, including in vitro imaging (e.g.,
POI imaging, cellular condition monitoring, microorganism imaging, system development), in vivo imaging,
biomolecule immobilization (e.g., POI collection, protein/nuclear acid interaction and protein structure analysis),
targeted degradation (e.g., L-AdPROM), and more. We also present a systematic discussion regarding the future
direction and challenges of the HaloTag technique. 相似文献
4.
5.
Four types of herring industry processing waters; refrigerated sea water (RSW), storage water (SW), processing water from cutting (PW) and pre-salting brines (SB) were subjected to chemical characterization and biomolecule recovery using electroflocculation (EF) and ultrafiltration (UF). The highest protein and fatty acid content were found in SB's, up to 12.7 ± 0.3 and 2.5 ± 0.1 g L−1, respectively. Long chain n-3 polyunsaturated fatty acids represented up to 44.5% of total fatty acids. In all samples, leucine and glutamic acid/glutamine were the dominating amino acids while calcium and magnesium were the dominating trace elements. EF plus UF in series recovered up to 80% proteins and fatty acids from SB's and reduced chemical oxygen demand by 70%. Foaming and emulsifying properties of biomolecules were improved or unaffected by EF/UF treatment. To conclude, large amounts of biomass are currently lost per ton of processed herring, e.g. ∼9.2 kg proteins and ∼4.1 kg fatty acids; EF/UF represents a promising way of turning such losses to a potential income. 相似文献
6.
Ilsoo Kim So-Eun Kim Sanghun Han Hyungsuk Kim Jaehyung Lee Du-Won Jeong Ju-Jin Kim Yong-beom Lim Heon-Jin Choi 《Nanoscale research letters》2013,8(1):502
Au-coated vertical silicon nanowire electrode array (VSNEA) was fabricated using a combination of bottom-up and top-down approaches by chemical vapor deposition and complementary metal-oxide-semiconductor process for biomolecule sensing. To verify the feasibility for the detection of biomolecules, Au-coated VSNEA was functionalized using peptides having a fluorescent probe. Cyclic voltammograms of the peptide-functionalized Au-coated VSNEA show a steady-state electrochemical current behavior. Because of the critically small dimension and vertically aligned nature of VSNEA, the current density of Au-coated VSNEA was dramatically higher than that of Au film electrodes. Au-coated VSNEA further showed a large current difference with and without peptides that was nine times more than that of Au film electrodes. These results indicate that Au-coated VSENA is highly effective device to detect peptides compared to conventional thin-film electrodes. Au-coated VSNEA can also be used as a divergent biosensor platform in many applications. 相似文献
7.
D.P. Xu S.-H. Yoon I. Mochida W.M. Qiao Y.G. Wang L.C. Ling 《Microporous and mesoporous materials》2008,115(3):461-468
Mesoporous carbon (MC) with high surface area and large pore volume was synthesized using mesophase pitch as a carbon precursor and nanosized MgO as an additive. The maximum surface area, largest pore volume and highest mesoporous ratio of as-prepared MC were up to 1400 m2/g, 2.8 cm3/g and 89%, respectively. The mesoporous structures (3–40 nm) of MC were directly observed under SEM and TEM. The adsorption capacity and adsorption rate of MC to vitamin B12 (VB), chicken egg white albumin (CEWA) and bovine serum albumin (BSA) were proportional to the mesopore volume and average pore size. MC (PM4-OC) exhibited the maximum adsorption capacity to the typical biomolecules, 486, 140 and 176 mg/g for VB, CEWA and BSA, respectively. In contrast, Maxsorbs (commercial activated carbons) with a surprising surface area gave a very low adsorption to such biomolecules. The research indicates that MC may be potential in the selective adsorption and separation of biomolecules, based on a molecule sieve effect. 相似文献
8.
Jungseung KimRebecca J. Desch Stephen W. Thiel Vadim V. GuliantsNeville G. Pinto 《Microporous and mesoporous materials》2012,149(1):60-68
Mesostructured cellular foam (MCF) silica was synthesized using a non-ionic surfactant template-directed method without ammonium fluoride; the acid concentration and aging time were varied to determine the effects of these parameters on the final material. Increasing the acid concentration and aging time resulted in larger window size, which is critical in gating of biomolecule access to the interior of the MCF silica. In particular, when the acid concentration was changed from 1.6 to 3.5 M the window pore dimension approximately doubled, although the pore size distribution was broader. In this study, the optimal synthesis conditions to produce large, narrowly distributed window pores are 3.5 M HCl with an aging time of 20 h. The loadings of l-tryptophan (Trp), lysozyme (LYS) and bovine serum albumin (BSA) on the MCF samples were measured using batch adsorption. Adsorption data followed a Type I isotherm. The monolayer adsorption capacity of Trp on acid-washed MCF was several times higher than that of LYS and BSA, because of the smaller size of Trp. Protein adsorption onto MCF silica showed minimal size exclusion until the window size of the silica was barely larger than the largest protein dimension. 相似文献
9.
Masataka Ohkubo Masahiro Ukibe Yiner Chen Shigetomo Shiki Yuki Sato Shigeo Tomita Shigeo Hayakawa 《Journal of Low Temperature Physics》2008,151(3-4):760-765
Superconducting detectors have no dead surface-layer. It has been found that even if there is a 700 nm-thick SiO2 layer on the sensitive area, the detectors produce measurable output pulses for molecule impact. This feature is very attractive
in solid-state spectroscopy of low-energy atoms or molecules for basic chemistry, nuclear physics, and life science. The superconducting
tunnel junction detectors enable the measurement of the deposited energy for individual particle impacts in contrast to conventional
particle detectors that rely on secondary particle emission. A study of the particle-surface interaction with atoms, proteins,
and synthetic polymers has revealed that there are three regions. As the mass value increases, the pulse height reduction,
or the decrease of the deposited energy, is remarkable in a mass range below 2,000, the pulse height increases in 2,000–100,000,
and finally almost constant pulse height appears in 100,000–1,000,000.
相似文献
10.
《International Journal of Hydrogen Energy》2020,45(7):3905-3915
In this study, we report the development of dihydropyrimidines substituted nickel electro-catalyst at mild conditions for hydrogen evolution reaction. The Biginelli type reaction was carried out to produce 4-(4-chlorophenyl)-3,4,5,6-tetrahydrobenzo[h]quinazoline-2(1H)-thione moiety. This was added with NiCl2.6H2O to synthesize Ni-quinazoline-2(1H)-thione catalyst. The prepared catalyst was characterized using UV–Vis, FT-IR, Mass, 1H and 13C NMR and SEM-EDX spectroscopy. It showed an excellent hydrogen evolution reaction (HER) activity. LSV polarization showed a low Tafel slope (34 mV/dec) nearly close to Pt/C (28 mV/dec) in neutral medium whereas a Tafel slope of 100 mV/dec was observed with 0.5M H2SO4 while Pt/C showed 50 mV/dec. Electrochemical impedance spectroscopy measured by applying −1.18 V in neutral medium showed two depressed semicircles whereas with 0.5M H2SO4 and -1.45 V applied voltage single half was observed. 相似文献