抗真菌蛋白Rs-AFP2基因定点突变及其对大肠杆菌中表达的影响

为了研究遗传密码子对表达调控的影响,利用PCR重叠延伸法,对萝卜抗真菌蛋白Rs-AFP2基因编码序列区的部分核苷酸进行沉默突变,构建突变体Rs-AFPm.序列分析表明,PCR产物全长240bp,有一个阅读框,编码的蛋白由29个氨基酸的信号肽和51个氨基酸的抗真菌蛋白组成.突变体与突变前的Rs-AFP2基因相比,在编码区第3号氨基酸Lys相差一个碱基(TTG→TTA),第5号氨基酸Gln相差一个碱基(CAG→CAA),第6号稀有密码子Arg相差两个碱基(CAG→CGA).重新合成引物,将切除信号肽的Rs-AFP2基因和Rs-AFPm基因与原核表达载体pET-21b(+)分别重组到大肠杆菌BL21菌株.IPTG诱导后,二者均得到了表达.软件分析显示,突变前pETAFPo表达产物占全菌蛋白的3%,突变后pETAFPm的表达产物占全菌蛋白含量的8%;表达蛋白主要以包涵体的形式存在,包涵体经超声波破碎后,蛋白质复性,抑菌结果表明,pETAFPm表达产物的抑菌半径大于pETAFP2表达产物的抑菌半径.这些都说明改造后的Rs-AFPm基因与Rs-AFP2基因相比,已有效地提高表达量.     

一类Feistel密码的线性分析

该文提出一种新的求取分组密码线性偏差上界的方法，特别适用于密钥线性作用的Feistel密码．该分析方法的思路是，首先对密码体制线性偏差进行严格的数学描述，分别给出密码线性偏差与轮函数F及S盒的线性偏差的数学关系；然后通过求取线性方程组最小重量解，确定密码线性偏差的上界．     

Biases of Success Rate Differences Shown in Binomial Effect Size Displays.

The intent of a binomial effect size display (BESD) is to show "the [real-world] importance of [an] effect indexed by a correlation [r]" (R. Rosenthal, 1994, p. 242) by reexpressing this correlation as a success rate difference (SRD) (e.g., treatment group success rate - control group success rate). However, SRDs displayed in BESDs generally overestimate real-world SRDs implied by correlations of (a) dichotomous X and Y variables (φ coefficients), (b) dichotomous X and continuous Y variables (point-biserial coefficients [rphs]). and (c) continuous X and Y variables (rxys). Furthermore, overestimation biases are larger for rxys than for rphs. Differences in the sizes of biases linked to different correlations suggest that BESD SRDs reported for different correlations are not comparable. The stochastic difference index (N. Cliff, 1993: A. Vargha & H. D. Delaney, 2000) is recommended as an alternative to the BESD. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

A novel compact circuit for 4-PAM energy-efficient high speed interconnect data transmission and reception

Transmission of signals, whether on-chip or off-chip, places severe constraints on timing and extracts a large price in energy. New silicon device technologies, such as back-plane CMOS, provide a programmable and adaptable threshold voltage as an additional tool that can be used for low power design. We show that one particularly desirable use of this freedom is energy-efficient high-speed transmission across long interconnects using multi-valued encoding. Our multi-valued CMOS circuits take advantage of the threshold voltage control of the transistors, by using the signal-voltage-to-threshold-voltage span, in order to make area-efficient implementations of 4-PAM (pulse amplitude modulation) transceivers operating at high speed. In a comparison of a variety of published technologies, for signal transmission with interconnects of 10-15 mm length, we show up to 50% improvement in energy for on-chip signal transmission over binary encoding together with higher limits for operating speeds without a penalty in circuit noise margin.     

Halo effects in grading student projects.

Halo effects in rating specific pieces of work, as in educational grading, have received little attention. Grades awarded by 2 independent graders to undergraduate projects were analyzed with a correlated uniqueness model. Grades showed substantial halo despite being awarded by expert assessors at the time of reading the work. There was greater halo between different grades applying to the same section of the project than between grades applying to different sections. Supervisors who had regular contact with the student whose work they were grading showed no more halo than other graders. More reliable graders showed less within-section halo than graders of lower reliability but equal between-sections halo. The halo effects observed cannot be entirely attributable to a unitary general impression. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

一种低压低功耗SRAM/SOI单元设计

提出一种改进4管自体偏压结构SRAM/SOI单元.基于TSUPREM4和MEDICI软件的模拟和结构性能的分析,设计单元结构并选取结构参数.该结构采用nMOS栅下的含p+埋沟的衬底体电阻代替传统6管CMOSSRAM单元中的pMOS元件,具有面积小、工艺简单的优点.该结构可以在0.5V的电源电压下正常工作,与6管单元相比,该单元瞬态响应正常,功耗只有6管单元的1/10,满足低压低功耗的要求.     

Evaluating reactivity to ecological momentary assessment during smoking cessation.

Ecological momentary assessment (EMA) consists of assessing phenomena in real time in the natural environment. EMA allows for more fine-grained analyses of addictive behavior and minimizes threats to internal validity, such as recall biases and errors. However, because of the intensive monitoring involved in EMA, measurement reactivity is a concern. To test whether EMA with palmtop personal computers induces reactivity, the authors compared smoking-related outcomes between smokers using EMA and those not using EMA during a quit attempt. The use of no-EMA control groups has been rare in reactivity investigations to date. The EMA protocol included event-contingent assessments (smoking episodes, urge episodes) and random assessments. Outcomes included biologically confirmed abstinence and self-report measures of withdrawal, self-efficacy, motivation, affect, and temptations. Participants were smokers motivated to quit (N = 96). They were randomized to 1 of 3 groups: EMA for the week preceding a planned quit date, EMA for the week following the quit date, and no EMA. Abstinence rates did not differ between the groups at Day 7 or at Day 28 postcessation. For the 20 subscales assessed at each of 3 assessment times, there were significant differences between participants with and without EMA experience for 3 subscales at the 1st of 3 assessment times, and significant differences for 3 different subscales at the 3rd assessment time. These differences suggest some reactivity to EMA, although the inconsistent pattern across time indicates that further research is needed to definitively conclude that EMA induces reactivity. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Self-controlled case series analyses: Small-sample performance

Second-order expressions for the asymptotic bias and variance of the log relative incidence estimator are derived for the self-controlled case series model in a simplified scenario. The dependence of the bias and variance on factors such as the relative incidence and ratio of risk to observation period are studied. Small-sample performance of the estimator in realistic scenarios is investigated using simulations. It is found that, in scenarios likely to arise in practice, asymptotic methods are valid for numbers of cases in excess of 20-50 depending on the ratio of the risk period to the observation period and on the relative incidence. The application of Monte Carlo methods to self-controlled case series analyses is also discussed.     

舰载雷达探测误差传递与灵敏度分析

在舰艇及编队指控系统中,来自不同平台的传感器探测系统偏差失配是影响态势一致性的关键因素。本文采用理论建模和数字仿真相结合的方法,对舰载雷达探测和处理涉及各环节的偏差进行分析,建立了雷达测量、坐标转换、平台定姿定位和基线转换等各环节偏差影响模型和舰载雷达探测的等效偏差模型。理论分析和计算机仿真结果表明,雷达方位测量、平台艏向和甲板艏向形变引起的偏差均可以直接迭加到方位上,合并为方位偏差;同时,在目标俯仰角小于10°的情况下,基本上可以忽略甲板形变、平台姿态偏差对目标方位测量的影响。     

工艺参数对射频磁控溅射PTFE靶形成的等离子体组成的影响

采用四极质谱仪测量了试验参数对高压脉冲增强射频磁控溅射PTFE靶等离子体气氛的影响规律。结果表明：增加脉冲偏压、脉冲频率、脉宽、射频功率和气压能提高Az离子对PTFE靶的溅射能力，增加空间中氟碳自由基的数量，其中各参数对峰位位于25．0aum处的氟碳自由基强度影响最大：脉冲电压从10kV提高到20kV能将该峰强度提高2倍；脉宽从40s提高到100μs强度提高80倍；频率从50Hz提高到200Hz强度提高4倍；溅射气压由0．1Pa提高到0.3Pa强度提高6倍；射频功率由200W提高到400W强度提高6倍。励磁电流能有效的约束氟碳自由基的空间分布。