TPS/TDC3000系统串口通讯的规划与应用

针对TPS/TDC30 0 0系统串口通讯方式、机理进行了一般性的介绍 ,对规划与应用中常见的的问题 ,根据实际工程应用经验提出了解决方案。     

DCS在30m~3聚合釜控制中的应用

介绍了电化厂聚合技改后的聚合釜温度控制方案,CS3000DCS在控制中的应用。实现了从进料、升温、恒温、直至反应结束的全过程自动控制,在操作站实现了动态工程流程图、控制分组、历史趋势等显示功能,可变参数在权限范围内的下载及报表打印等功能。     

SC-3000智能均流测试仪在整流供电系统中的应用

论述了均流仪在整流机组均流管理中的功效.     

SCAN 3000系统在单质起爆药生产的应用

介绍了在单质起爆药生产中SCAN3000系统的基本构成、系统功能以及系统的冗余设计。通过对控制图和梯形逻辑图的组态，实现了控制方案。     

A．MT．3000断面测量系统及其应用

Ａ．ＭＴ．３０００断面测量系统及其在二滩水电工程中的应用证明，该系统简比了断面测量方法，能有效地提高工作效率，在水电等工程建设中具有明显的经济效益。     

μC／OS—Ⅲ在Cortex—M3处理器上的移植

为了将μC／OS-Ⅲ移植到Cortex—M3处理器上,选用RealView MDK作为软件开发平台,针对Cortex—M3处理器特性编写了移植所需的C语言和汇编语言源代码,并验证了移植的正确性。移植后的μC／OS-Ⅲ能够稳定运行于Cortex～M3处理器上。该移植对大部分Cortex—M3处理器具有通用性,对其他架构处理器的μC／OS-Ⅲ移植具有参考作用。     

Proliferation to Apoptosis Tumor Cell Ratio as a Biomarker to Improve Clinical Management of Pre-Malignant and Symptomatic Plasma Cell Neoplasms

Proliferation and apoptosis of neoplastic cells are prognostic biomarkers in plasma cell neoplasms (PCNs). The prognostic capacity of proliferation to apoptosis ratio (Ratio-PA) in the era of immunomodulatory treatments is re-evaluated in 316 gammopathy of undetermined significance (MGUS), 57 smoldering multiple myeloma (SMM), and 266 multiple myeloma (MM) patients. Ratio-PA of 0.77 ± 0.12, 1.94 ± 0.52, and 11.2 ± 0.7 (p < 0.0001) were observed in MGUS, SMM, and MM patients. Ten-year overall survival (10y-OS) rates for patients with low/high Ratio-PA were 93.5%/77.3% p < 0.0001) for MGUS, 82.5%/64.7% (p < 0.05) for SMM, and 62.3%/47.0% (p < 0.05) for MM. For patients with low, intermediate, and high risk, 10y-OS for low/high Ratio-PA were 95.5%/72.9% (p < 0.0001), 74.2%/50.4% (p < 0.0001), and 35.3%/20.0% (p = 0.836), respectively. Ratio-PA was an independent prognostic factor for OS (HR = 2.119, p < 0.0001, Harrell-C-statistic = 0.7440 ± 0.0194) when co-analyzed with sex, age, and standard risk. In patients with Ratio-PA^high, only first-line therapy with VRd/VTd, but not PAD/VCD, coupled with ASCT was associated with high 10y-OS (82.7%). Tumor cell Ratio-PA estimated at diagnosis offers a prognostic biomarker that complements standard risk stratification and helps to guide the clinical management of pre-malignant and symptomatic PCNs. Every effort should be made to provide first-line therapies including VTd or VRd associated with ASCT to patients with Ratio-PA^high at higher risk of progression and death.     

微波诱导稀土复合固体超强酸SO4^2-／ZrO2-Nd2O3催化合成乙酸苄酯

以新型稀土复合固体超强酸为催化剂，在微波辐射下苯甲醇和乙酸反应合成了乙酸苄酯，探索反应各因素对酯化率的影响。实验结果表明：SO4^2-／ZrO2-Nd2O3具有较高的催化活性。最佳反应条件为：醇酸摩尔比2．0（乙酸用量为0．2mol前提下），催化剂用量为1．8g，带水剂环己烷12mL，微波辐射功率550W，辐射时间25min，酯化率可达96．3％。该催化剂易于回收且可重复使用，具有良好的活性稳定性。     

Combination of computational prescreening and experimental library construction can accelerate enzyme optimization by directed evolution

Chiral compounds can be produced efficiently by using biocatalysts. However, wild-type enzymes often do not meet the requirements of a production process, making optimization by rational design or directed evolution necessary. Here, we studied the lipase-catalyzed hydrolysis of the model substrate 1-(2-naphthyl)ethyl acetate both theoretically and experimentally. We found that a computational equivalent of alanine scanning mutagenesis based on QM/MM methodology can be applied to identify amino acid positions important for the activity of the enzyme. The theoretical results are consistent with concomitant experimental work using complete saturation mutagenesis and high-throughput screening of the target biocatalyst, a lipase from Bacillus subtilis. Both QM/MM-based calculations and molecular biology experiments identify histidine 76 as a residue that strongly affects the catalytic activity. The experiments demonstrate its important influence on enantioselectivity.     

A QM/MM study of the catalytic mechanism of aspartate ammonia lyase

Aspartate ammonia lyase (Asp) is one of three types of ammonia lyases specific for aspartate or its derivatives as substrates, which catalyzes the reversible reaction of l-aspartate to yield fumarate and ammonia. In this paper, the catalytic mechanism of Asp has been studied by using combined quantum-mechanical/molecular-mechanical (QM/MM) approach. The calculation results indicate that the overall reaction only contains two elementary steps. The first step is the abstraction of C_β proton of l-aspartate by Ser318, which is calculated to be rate limiting. The second step is the cleavage of C_αN bond of l-aspartate to form fumarate and ammonia. Ser318 functions as the catalytic base, whereas His188 is a dispensable residue, but its protonation state can influence the active site structure and the existing form of leaving amino group, thereby influences the activity of the enzyme, which can well explain the pH dependence of enzymatic activity. Mutation of His188 to Ala only changes the active site structure and slightly elongates the distance of C_β proton of substrate with Ser318, causing the enzyme to remain significant but reduced activity.