直接和间接的笔倾斜输入性能对比

越来越多的笔式设备通过增加笔倾斜输入来提高其输入能力。但在直接输入设备和间接输入设备上执行笔的倾斜操作时,由于操作环境和视觉反馈的不同,往往会导致操作性能产生差异。本研究以探索笔倾斜输入在直接设备和间接设备上的性能差异为目的,以实证手段考察在两种设备上获取不同角间距下的不同笔倾斜目标时的操作速度、稳定性和准确性,讨论导致直接设备和间接设备笔倾斜性能不同的原因,并提出适用的角间距和倾斜目标位置。实验结果表明,在直接设备和间接设备上操作笔倾斜角时,在操作速度上直接设备优于间接设备,但在稳定性和准确性上,间接设备优于直接设备。20°的角间距对两种设备来说都呈现较好的性能,若需要在更小角间距内完成目标选择操作,在间接设备上的效果更好。最后,对两种设备的用户使用习惯进行了总结,为基于笔倾斜角的用户界面设计提供了帮助和参考。     

Secretome analysis of novel IgE-binding proteins from Penicillium citrinum

The Penicillium genus of fungi is a frequently reported cause of allergic reactions. However, only a limited number of allergens have been reported. In Penicillium spp., many allergens show higher IgE-binding activity in culture filtrate extracts than in cellular extracts. In order to investigate the IgE-reactive profile of mold-sensitized patients, secreted IgE-reactive proteins from Penicillium citrinum were identified by 2-DE, serum immunoblotting, and nanoLC-MS/MS. Among the IgE-reactive spots, one known allergen, Pen c 13, and four novel allergens were identified. The cDNAs coding for Pen c 32 and Pen c 30 were cloned using designed primers based on nanoLC-MS/MS analysis. The amino acid sequences of Pen c 32 and Pen c 30 were, respectively, found to have extensive similarity with those of pectate lyases and catalases from various fungi. Native Pen c 30 was shown to have catalase activity and to bind to serum IgE from 48% of mold-allergic patients and induced immediate type skin reactions in a sensitized patient. Here, we present a proteome approach which resulted in the identification of four novel secreted allergens. These novel allergens might be useful in allergy diagnosis and in the treatment of mold-allergic disorders.     

虾过敏原Troponin C (Pen m6)的克隆表达、免疫学鉴定及生物信息学分析

目的对斑节对虾(Penaeus monodon)第6组过敏原(Pen m6),肌钙蛋白C(Troponin C)进行克隆表达、免疫学鉴定并研究其生物学意义。方法提取斑节对虾总RNA;根据Gen Bank:HM034316.1设计引物及构建表达载体PET-24a-Pen m6;转化至大肠杆菌BL21(DE3)进行表达;纯化后的重组Pen m6用Western bolt来鉴定免疫学特性;用生物信息学相关工具对Pen m6进行同源性分析,并预测其蛋白质的结构和功能。结果克隆出斑节对虾Pen m6基因开放阅读框453 bp,编码150个氨基酸。重组Pen m6蛋白呈可溶性,分子量约35kDa,能够与斑节对虾过敏患者血清IgE结合。斑节对虾与凡纳滨对虾亲缘关系比较近,其中斑节对虾Pen m6蛋白与凡纳滨对虾Troponin C1(gb|AET36896.1|)同源性为98%。理化性质预测Pen m6蛋白质不稳定。蛋白质结构预测结果显示Pen m6的结构主要以α螺旋组成。T细胞抗原表位预测得到4个肽序列(15~23、35~43、91~99、112~120)。B细胞抗原表位预测得到4个肽序列(7~16、21~30、28~37、58~67)。结论克隆的重组斑节对虾Pen m6蛋白具有良好的免疫原性,为进一步研究斑节对虾过敏原的结构成分及其理化性质奠定理论基础。     

Control of line width with active nano fountain pen (ANFP) for nano manufacturing

In this paper, modeling of ANFP (active nano fountain pen) and a nano size line control system with ANFP based on fuzzy algorithm is developed. It was found that line width is affected directly with initial mass of meniscus; velocity of patterning and concentration of ink directly affect line width. The control algorithm using the result of modeling successfully shows that the speed of patterning is increased and a variation of line width is possible compared with DPN (dip pen nano lithograph) and FPN (fountain pen nano lithograph). The control of line width system with ANFP is developed by using fuzzy algorithm. Finally, it is shown that it is possible to develop an effective control of variant line width with the same speed.     

模拟肠液对Pen a1及其抗原表位免疫原性的影响

体外模拟人体肠液(SIF)消化分析Pen a1消化后免疫原性的变化规律。虾致敏蛋白Pen a1及其表位多肽经SIF消化后,用全抗体和表位特异性抗体检测Pen a1及其各抗原表位的免疫原性的变化,并测定表位多肽的SIF消化稳定性。结果表明,Pen a1的免疫原性在消化60 min内下降显著,在90 min后下降缓慢,生成的新片段仍具有免疫原性,但会逐步完全分解至SDS-PAGE无法检测出;Pen a1中5个抗原表位的免疫原性变化也类似。Western-blot表明五个表位抗体与生成的新蛋白结合程度不同,No.3、4、5抗体与20~30 ku处的新蛋白片段结合多于No.1、2。ELISA检测表明即使经过4 h的消化,免疫原性也只是降低了80%。Pen a1各表位消化稳定性为No.2No.1No.3No.4No.5;5个表位多肽的消化稳定性为No.3No.1No.4No.2No.5,与各表位上的酶切位点的个数呈负相关。可以得出Pen a1中No.2表位具有最高的消化稳定性,No.5表位表消化稳定性最差。     

螺旋榨油机榨排的设计计算

设计计算榨排时如何选择材料、类型和结构作了简要述叙，对榨排的受力进行了分析，同时对不同榨排进行了强度计算和校核。     

Patterning High Explosives at the Nanoscale

For the first time, we have shown that spin coating and Dip pen nanolithography (DPN^TM) are simple methods of preparing energetic materials such as PETN and HMX on the nanoscale, requiring no heating of the energetic material. Nanoscale patterning has been demonstrated by the DPN method while continuous thin films were produced using the spin coating method. Results are presented for preparing continuous PETN thin films of nanometer thickness by the spin coating method and for controlling the architecture of arbitrary nanoscale patterns of PETN and HMX by the DPN method. These methods are simple for patterning energetic materials and can be extended beyond PETN and HMX, opening the door for fundamental studies at the nanoscale.     

彩色水笔染料鉴别

用平面色谱法鉴别彩色水笔染料。欧美产品多用水溶性食用色素配色，无毒安全，可供借鉴。国内产品多用工业染料，似需改进。     

Hybrid ternary rice paper/polypyrrole ink/pen ink nanocomposites as components of flexible supercapacitors

The rapid development of the portable and wearable devices has inspired the ever-growing pursuit of flexible energy storage equipment which can power these devices. Here, rice paper (RP) was integrated with a homemade LGS-polypyrrole (LGS-PPy) ink and commercial pen ink in order to construct a flexible electrode of a supercapacitor via a facial dip-coating method. The obtained RP/LGS-PPy ink/Pen ink composites showed a high areal specific capacitance of 1568 mF/cm² at 0.2 mA/cm², owing to the uniform deposition of a LGS-PPy layer which was covered by the pen ink coating. Furthermore, the assembled symmetric supercapacitor fabricated from the RP/LGS-PPy ink/Pen ink electrode exhibited impressive electrochemical performances in terms of specific capacitance (317.5 mF/cm² at 2 mA/cm²), power density (846 μW/cm² at the energy density of 23.5 μWh/cm²), and life times (82.1% capacitance retention after 5000 cycles). In addition, the capacitance of the as-prepared device remained essentially unchanged even after bending at 180°, thus demonstrating this device's outstanding flexibility. The low cost of raw materials, robust fabrication strategy as well as the moderate performances make the RP-based supercapacitor a promising candidate for future flexible energy storage devices.