Comparative analysis of inbreeding parameters and runs of homozygosity islands in 2 Italian autochthonous cattle breeds mainly raised in the Parmigiano-Reggiano cheese production region

Reggiana and Modenese are autochthonous cattle breeds, reared in the North of Italy, that can be mainly distinguished for their standard coat color (Reggiana is red, whereas Modenese is white with some pale gray shades). Almost all milk produced by these breeds is transformed into 2 mono-breed branded Parmigiano-Reggiano cheeses, from which farmers receive the economic incomes needed for the sustainable conservation of these animal genetic resources. After the setting up of their herd books in 1960s, these breeds experienced a strong reduction in the population size that was subsequently reverted starting in the 1990s (Reggiana) or more recently (Modenese) reaching at present a total of about 2,800 and 500 registered cows, respectively. Due to the small population size of these breeds, inbreeding is a very important cause of concern for their conservation programs. Inbreeding is traditionally estimated using pedigree data, which are summarized in an inbreeding coefficient calculated at the individual level (F_PED). However, incompleteness of pedigree information and registration errors can affect the effectiveness of conservation strategies. High-throughput SNP genotyping platforms allow investigation of inbreeding using genome information that can overcome the limits of pedigree data. Several approaches have been proposed to estimate genomic inbreeding, with the use of runs of homozygosity (ROH) considered to be the more appropriate. In this study, several pedigree and genomic inbreeding parameters, calculated using the whole herd book populations or considering genotyping information (GeneSeek GGP Bovine 150K) from 1,684 Reggiana cattle and 323 Modenese cattle, were compared. Average inbreeding values per year were used to calculate effective population size. Reggiana breed had generally lower genomic inbreeding values than Modenese breed. The low correlation between pedigree-based and genomic-based parameters (ranging from 0.187 to 0.195 and 0.319 to 0.323 in the Reggiana and Modenese breeds, respectively) reflected the common problems of local populations in which pedigree records are not complete. The high proportion of short ROH over the total number of ROH indicates no major recent inbreeding events in both breeds. ROH islands spread over the genome of the 2 breeds (15 in Reggiana and 14 in Modenese) identified several signatures of selection. Some of these included genes affecting milk production traits, stature, body conformation traits (with a main ROH island in both breeds on BTA6 containing the ABCG2, NCAPG, and LCORL genes) and coat color (on BTA13 in Modenese containing the ASIP gene). In conclusion, this work provides an extensive comparative analysis of pedigree and genomic inbreeding parameters and relevant genomic information that will be useful in the conservation strategies of these 2 iconic local cattle breeds.     

数字签名技术在医院电子病历中的设计与应用

为推进病历数字化发展,并确保其信息的安全性,将以HIS电子病历系统为基础,采用安信数字签名技术和PKI或PMI系统搭建相信并加以任用的授权服务,经过针对实际的PKC和CA的确认、委托与管控构建整体的数字签名平台,完成电子病历数字签名功能设计,以加强电子文件的完整性、真实性和不可抵赖性。最后以某医院的XML结构化的电子病历系统为基础进行项目实施,完成了医护人员通过HIS的快速身份认证,和准确地数字签名。     

匿名技术的研究进展

对匿名技术的研究情况进行了综述。统一给出了关联性、匿名性、假名等概念,探讨了Crowds、洋葱路由、Mix nets等匿名通信的实现机制,介绍了有关的研究进展,指出了匿名性度量、环签名和群签名等匿名签名算法、匿名应用中信赖的建立等技术中需要进一步研究的问题。     

一个安全有效的身份鉴别协议与对应的数字签名方案

本文利用二元一次不定方程和Schnorr身份鉴别协议，构造出一个新的身份鉴别协议，该协议在RSA问题和离散对数问题是难解的假设下是安全的，同时给出了所对应的数字签名方案。     

Trade-offs in scan path and BIST implementations for RAMs

In this article we propose efficient scan path and BIST schemes for RAMs. Tools for automatic generation of these schemes have been implemented. They reduce the design effort and thus allow the designer to select the more appropriate scheme with respect to various constraints.     

Zero aliasing ROM BIST

Signature analyzers are very efficient output response compactors for BIST design. The only limitation of signature analysis is the fault coverage reduction (aliasing) due to the information loss inherent to any data compaction. In this article, in order to increase the effectiveness of ROM BIST, we take advantage from the simplicity of the error patterns generated by ROMs and we show that aliasing free signature analysis can be achieved in ROM BIST.This work was performed when the author was on leave from Minsk Radio Engineering Institute, Computer Department, Belorus.     

Mixed-Signal Circuit Classification in a Pseudo-Random Testing Scheme

Pseudo-random testing techniques for mixed-signal circuits offer several advantages compared to explicit time-domain and frequency-domain test methods, especially in a BIST structure. To fully exploit these advantages a suitable choice of the pseudo-random input parameters should be done and an investigation on the accuracy of the circuit response samples needed to reduce the risk of misclassification should be carried out. Here these issues have been addressed for a testing scheme based on the estimation of the impulse response of the device under test (DUT) by means of input-output cross-correlation. Moreover, new acceptance criteria for the DUT are suggested which solve some ambiguity problems arising if the classification of the DUT as good or bad is based on a few samples of the cross-correlation function. Examples of application of the proposed techniques to real cases are also shown in order to assess the impact of the measurement system inaccuracies on the reliability of the test.     

基于媒体用户访问行为偏好模型的代理缓存算法

目前,代理缓存技术广泛应用于改善流媒体传输的服务质量.文章从实际用户日志文件的分析出发,利用发现的用户浏览流媒体对象时的行为分布模型,提出了一种新的视频流媒体缓存算法.仿真结果证明,该算法可以通过记录很少的用户访问信息获取较高的性能表现.     

两个基于身份的数字签名方案的安全性改进

分析了文献[1]提出的两个基于用户身份的数字签名方案，发现其一般签名方案存在安全性缺陷，在此基础上建立了多重签名方案，但此方案也是不安全的。本文给出了改进的签名和多重签名方案。     

Improvement on threshold proxy signcryption scheme

Signcryption is a public key or asymmetric cryptographic method that provides simultaneously both message confidentiality and integrity at a lower computational and communication cost. This paper presents a （t, n） threshold proxy signcryption scheme in which no less than t proxy agents in the proxy group can produce the signcryption cryptogram. combines the functionality of threshold proxy signing and encryption. The proposed scheme combines the functionality of both threshold proxy signing and encryption, and provides fair protection for the sender and the proxy agents. This scheme is secure against attack of public key substitution. It also satisfies security properties such as confidentiality and authenticity.