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排序方式: 共有66条查询结果,搜索用时 15 毫秒
1.
Michael H. Guerra Thangal Yumnamcha Abdul-Shukkur Ebrahim Elizabeth A. Berger Lalit Pukhrambam Singh Ahmed S. Ibrahim 《International journal of molecular sciences》2021,22(9)
Disruption of retinal pigment epithelial (RPE barrier integrity is a hallmark feature of various retinal blinding diseases, including diabetic macular edema and age-related macular degeneration, but the underlying causes and pathophysiology are not completely well-defined. One of the most conserved phenomena in biology is the progressive decline in mitochondrial function with aging leading to cytopathic hypoxia, where cells are unable to use oxygen for energy production. Therefore, this study aimed to thoroughly investigate the role of cytopathic hypoxia in compromising the barrier functionality of RPE cells. We used Electric Cell-Substrate Impedance Sensing (ECIS) system to monitor precisely in real time the barrier integrity of RPE cell line (ARPE-19) after treatment with various concentrations of cytopathic hypoxia-inducing agent, Cobalt(II) chloride (CoCl2). We further investigated how the resistance across ARPE-19 cells changes across three separate parameters: Rb (the electrical resistance between ARPE-19 cells), α (the resistance between the ARPE-19 and its substrate), and Cm (the capacitance of the ARPE-19 cell membrane). The viability of the ARPE-19 cells and mitochondrial bioenergetics were quantified with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and seahorse technology, respectively. ECIS measurement showed that CoCl2 reduced the total impedance of ARPE-19 cells in a dose dependent manner across all tested frequencies. Specifically, the ECIS program’s modelling demonstrated that CoCl2 affected Rb as it begins to drastically decrease earlier than α or Cm, although ARPE-19 cells’ viability was not compromised. Using seahorse technology, all three concentrations of CoCl2 significantly impaired basal, maximal, and ATP-linked respirations of ARPE-19 cells but did not affect proton leak and non-mitochondrial bioenergetic. Concordantly, the expression of a major paracellular tight junction protein (ZO-1) was reduced significantly with CoCl2-treatment in a dose-dependent manner. Our data demonstrate that the ARPE-19 cells have distinct dielectric properties in response to cytopathic hypoxia in which disruption of barrier integrity between ARPE-19 cells precedes any changes in cells’ viability, cell-substrate contacts, and cell membrane permeability. Such differences can be used in screening of selective agents that improve the assembly of RPE tight junction without compromising other RPE barrier parameters. 相似文献
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Borg's rating of perceived exertion (RPE) and category ratio (CR‐10) scales are commonly used to quantify perceived muscular exertion for body segments. Twenty females participated in an experiment to study the power grip force at four perceived exertion levels using either dominant or nondominant hand under two posture conditions. It was found that the subjects tended to apply a higher power grip force (100% of perceived maximum voluntary contraction) than the levels they were requested to apply. The power grip forces between dominant and nondominant hands at low hand exertion levels were negligible. The grip forces between the two hands were significantly different when the exertion level was nearly maximal. Linear regression models were established for the subjects to link the relationship between the perceived hand exertion and measured grip force, hand used, and hand/arm posture. All the models were statistically significant (p < 0.0001) with R2 values 0.97 or higher. These models provided better estimates in perceived hand exertion for dominant hand than for nondominant hand. A follow‐up experiment was conducted to measure the subjective rating of both the CR‐10 and RPE when a 98 N grip force was applied. It was found that the subjects reported higher exertion levels when they were using the CR‐10 scale than when they were using the RPE scale. © 2011 Wiley Periodicals, Inc. 相似文献
4.
介绍了一种在测量系统中使用的基于递推预报误差算法的前馈神经网络的设计以及实现方法。将该网络应用于测量系统模型的仿真试验取得了良好的效果。文中给出了试验的结果 ,并对该网络的应用进行了讨论 相似文献
5.
Behnam Noorani Fatemeh Yazdian Zahra-Soheila Soheili Mehdi Shakibaie Shiva Rahmani 《国际聚合物材料杂志》2018,67(12):754-763
Damage of the retinal pigmented epithelial cells causes various diseases such as age-related macular degeneration in retinal tissue. Nowadays, scientists are attempting to replace lost retinal cells with healthy and efficient cells that provide better conditions for recovering and preventing blindness. In this study, gelatin/chitosan nanofibrous scaffolds with mean diameters of 180?nm were fabricated for subretinal space through electrospinning. Thickness and morphology of the gelatin–chitosan scaffolds were analyzed by scanning electron microscopy (SEM). The results showed that the high rate of degradation, i.e., 90% damage was obtained after 1 month. The cell viability of gelatin/chitosan nanofibers were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The SEM results of cultured RPE on gelatin/chitosan scaffolds showed the appropriate adhesion of cells on the substrate. The results of the identity of RPE cells cultured on the scaffolds indicated that a large number of cells strongly expressed RPE65 and cytokeratin 8/18. 相似文献
6.
Li-Bo Cheng Chun-Ming Chen Hong Zhong Li-Juan Zhu 《International journal of molecular sciences》2014,15(10):18762-18775
Reactive oxygen species (ROS)-mediated retinal pigment epithelium (RPE) cell apoptosis is attributed to age-related macular degeneration (AMD) pathogenesis. FLZ, a novel synthetic squamosamide derivative from a Chinese herb, Annona glabra, has displayed significant cyto-protective activity. In the current study, we explored the pro-survival effect of FLZ in oxidative stressed-RPE cells and studied the underlying signaling mechanisms. Our results showed that FLZ attenuated hydrogen peroxide (H2O2)-induced viability decrease and apoptosis in the RPE cell line (ARPE-19 cells) and in primary mouse RPE cells. Western blotting results showed that FLZ activated AKT signaling in RPE cells. The AKT-specific inhibitor, MK-2206, the phosphoinositide 3-kinase (PI3K)/AKT pan inhibitor, wortmannin, and AKT1-shRNA (short hairpin RNA) depletion almost abolished FLZ-mediated pro-survival/anti-apoptosis activity. We discovered that epidermal growth factor receptor (EGFR) trans-activation mediated FLZ-induced AKT activation and the pro-survival effect in RPE cells, and the anti-apoptosis effect of FLZ against H2O2 was inhibited by the EGFR inhibitor, PD153035, or by EGFR shRNA-knockdown. In conclusion, FLZ protects RPE cells from oxidative stress through activation of EGFR-AKT signaling, and our results suggest that FLZ might have therapeutic values for AMD. 相似文献
7.
Celina Cziepluch Elisabeth Kordes Aurora Pujol Jean-Claude Jauniaux 《Yeast (Chichester, England)》1996,12(14):1471-1474
The complete sequence of a 40 247 bp DNA segment located on the left arm of chromosome X of Saccharomyces cerevisiae has been determined and analysed. The sequence encodes the 5′ coding region of the URA2 gene and 18 open reading frames of at least 100 amino acids. Ten of these correspond to known genes, whereas eight correspond to new genes. In addition, the sequence contains a tRNA-Ala gene, a tRNA-Asp gene, a Ty4 transposable element and three delta elements. The sequence has been deposited in the EMBL databank under Accession Numbers: Z49405, Z49404, Z49403, Z49402, Z49401, Z49400, Z49399, Z49398, Z49397, Z49396, Z49394, Z49392, Z49391, Z49390, Z49389, Z49387, Z49386, Z49385. 相似文献
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基于BP-ANN的热电偶信息处理方法 总被引:2,自引:1,他引:1
为了提高传感器的准确度,提出一种基于BP网络和递推预报误差算法对热电偶进行信息处理的方法,经过仿真试验证明该方法可以提高传感器在较大范围内的测量准确度。该方法用软件容易实现,可以很方便地应用到其它传感器中。 相似文献
10.
Age-related macular degeneration (AMD) is the major reason for blindness in the industrialized world with limited treatment options. Important pathogenic pathways in AMD include oxidative stress and vascular endothelial growth factor (VEGF) secretion. Due to their bioactivities, fucoidans have recently been suggested as potential therapeutics. This review gives an overview of the recent developments in this field. Recent studies have characterized several fucoidans from different species, with different molecular characteristics and different extraction methods, in regard to their ability to reduce oxidative stress and inhibit VEGF in AMD-relevant in vitro systems. As shown in these studies, fucoidans exhibit a species dependency in their bioactivity. Additionally, molecular properties such as molecular weight and fucose content are important issues. Fucoidans from Saccharina latissima and Laminaria hyperborea were identified as the most promising candidates for further development. Further research is warranted to establish fucoidans as potential therapeutics for AMD. 相似文献