Identification of SYS1 as a Host Factor Required for Shiga Toxin-Mediated Cytotoxicity in Vero Cells

Shiga toxin (STx) or Vero toxin is a virulence factor produced by enterohemorrhagic Escherichia coli. The toxin binds to the glycosphingolipid globotriaosylceramide (Gb3) for its entry, and causes cell death by inhibiting ribosome function. Previously, we performed a loss-of-function screen in HeLa cells using a human CRISPR knockout (KO) library and identified various host genes required for STx-induced cell death. To determine whether this library targeted to the human genome is applicable to non-human primate cells and to identify previously unrecognized factors crucial for STx-induced cell death, we herein performed a similar screen in the African green monkey kidney-derived Vero C1008 subline. Many genes relevant to metabolic enzymes and membrane trafficking were enriched, although the number of enriched genes was less than that obtained in the screening for HeLa cells. Of note, several genes that had not been enriched in the previous screening were enriched: one of these genes was SYS1, which encodes a multi-spanning membrane protein in the Golgi apparatus. In SYS1 KO Vero cells, expression of Gb3 and sphingomyelin was decreased, while that of glucosylceramide and lactosylceramide was increased. In addition, loss of SYS1 inhibited the biosynthesis of protein glycans, deformed the Golgi apparatus, and perturbed the localization of trans-Golgi network protein (TGN) 46. These results indicate that the human CRISPR KO library is applicable to Vero cell lines, and SYS1 has a widespread effect on glycan biosynthesis via regulation of intra-Golgi and endosome–TGN retrograde transports.     

基于多核DSP的以太网通信接口设计

针对8核DSP TMS320C6678与外部设备进行数据通信的需求,以片上集成千兆以太网交换子系统为核心,选取芯片88E1111作为PHY设备,设计了千兆以太网通信接口的硬件电路。在嵌入式操作系统SYS/BIOS和网络开发环境NDK上,完成了以太网底层驱动和TCP/IP协议的程序设计。通过DSP与上位机进行以太网通信测试,证明了以太网接口电路硬件及软件的正确性和实用性。     

K6型单层网壳极限承载力对杆件失效的灵敏度分析

基于Frangopol提出的冗余度计算方法和考虑双重非线性的全过程分析方法,应用有限元软件ANSYS对一个K6型单层网壳进行了极限承载力灵敏度分析,得出了该类型单层网壳的极限承载力对于杆件失效的灵敏度值。在此基础上,提出了一种防止网壳连续倒塌的有效措施,即加强具有低冗余度（高灵敏度）的杆件以提高其冗余度,从而降低整个单层网壳结构连续倒塌的概率。     

基于ANSYS的岩锚悬臂吊车梁结构数值分析

结合白山水电站三期扩建工程实际,将ANSYS应用于地下厂房岩锚吊车梁结构数值分析中,全面考虑混凝土、围岩和锚杆三者的变形对岩锚悬臂吊车梁受力状况的影响,利用三维非线性有限元技术仿真模拟岩锚吊车梁在运行期的荷载作用,分析岩锚吊车梁的工作特性和稳定性,为岩锚吊车梁的设计理论和施工方法研究提供有价值参数。     

基于TMS320C6748和SYS/BOIS的红外实时信号处理系统

针对红外实时信号处理系统的高性能、小型化、低功耗的工程需求,设计了一种基于DSP+FPGA红外实时信号处理系统。介绍了TI公司的TMS320C6748DSP和SYS/BOIS操作系统内核。给出了系统的详细硬件设计,简单介绍了系统的软件设计,实现了两点校正红外图像非均匀性校正算法和SB/MHT多目标跟踪算法。实验证明,该设计可满足红外信号处理系统的实时性要求。     

Novell网络使用中的若干问题及对策

主要阐述 Novell局域网在使用中经常遇到的一些问题 ,如脱网、SYS卷丢失、网卡、显卡和内存条故障以及应用软件 WT在网络上使用等问题 ,对它们进行分析 ,结合自己在工作中的经验 ,提出可行的解决办法。     

基于ANSYS的永磁同步电机设计分析软件

开发了永磁同步电机性能分析与设计软件。该软件通过参数化设计语言APDL，建立了有限元计算软什ANSYS和C＋＋设计程序之间的接口。使用VC＋＋6．0对ANSYS分析永磁同步电机内部电磁场进行二次开发，用户只需在设计分析软件界面中输入参数，系统即能自动调用ANSYS在后台进行计算，得到所需要的数据和图形。该软件系统具有友好、方便、易用的人机交互界面，对复杂的、难于理解和掌握的ANSYS命令流进行后台封装，因此，可让即使从未学习过ANSYS软件的工程设计人员也能很好地借助本系统进行永磁同步电机电磁场有限元分析。     

Development and verification of a soil–water coupled finite deformation analysis based on u–w–p formulation with fluid convective nonlinearity

Most soil–water coupled analyses of saturated soil are based on the u–p formulation, where a set of equations is reduced by assuming that the acceleration of the fluid phase relative to that of the solid phase is less than that of the solid phase. Therefore, this analysis cannot be used for a coupled analysis with dynamic water flow in highly permeable soil. This study aims to present a soil–water coupled finite deformation analysis method based on full formulation, or u–w–p formulation. This method differs from conventional methods in the following ways: (1) the governing equations explicitly include the equation of motion for the fluid phase, (2) a relative convective term is used to describe a change in the relative configuration between the two phases, and (3) the moving/inclined discharge boundary is directly implemented to the discretized governing equations. Herein, one/two dimensional seepage and plane-strain deformation analysis results are reported. In the seepage analysis, accelerating permeation of pore water is obtained and the undrained constraint condition is verified. In the deformation analysis, dynamic migration in a high permeable soil specimen, i.e., wave propagation and rotational flow of pore water, is observed.     

高速互联网网络驱动程序的虚拟ARP实现

该文在高速互联网系统所提供的MP(消息传递)机制基础上,提出并实现了以太网卡驱动程序的地址解析协议ARPSERVER机制。文中详细描述了ARPSERVER的工作原理,采取了冗余措施和Client主动汇报机制保证网络系统的正确和可靠性。并给出了具体的实现过程。     

PC系列微机内存的有效利用

本文论述了ＰＣ系列微机内存的空间分布及三种内存管理规范，并给出在ＤＯＳ５．０下有效地利用实际配置的大容量内存的方法。