Simultaneous HPLC quantification of total cholesterol,tocopherols and β-carotene in Barrosã-PDO veal

A simple, rapid and sensitive procedure for the simultaneous determination of total cholesterol, tocopherols and β-carotene in meat is described. The method involves a direct saponification of the meat, a single n-hexane extraction and the analysis of the extracted compounds by normal-phase HPLC, using fluorescence (tocopherols) and UV–Vis photodiode array (cholesterol and β-carotene) detections in tandem. Rates of recovery of spiked meat samples were 93% for cholesterol, 83–86% for (α-, β- and -γ) tocopherols and 89% for β-carotene. Repeatabilities were high (CV < 6%) for all determined compounds, except for δ-tocopherol. This tocopherol, which is not usually present in meat, showed a much lower recovery percentage (73%) and repeatability (12.8%). This methodology was applied for the quantification of total cholesterol, tocopherols and β-carotene in three muscles (longissimus thoracis, longissimus lumborum and semitendinosus) of the Portuguese traditional Barrosã-PDO veal, obtained from autochthonous calves fed extensively during summer (with the least abundant green pastures) and slaughtered in early autumn (October). Barrosã-PDO veal showed median contents of total cholesterol (0.50–0.56 mg/g) and, depending on the analysed muscle, moderate to high contents of α-tocopherol (3.3–3.9 μg/g) and β-carotene (0.07–0.09 μg/g), suggesting an high sensorial and hygienic quality.     

Confocal Blood Flow Videomicroscopy of Thrombus Formation over Human Arteries and Local Targeting of P2X7

Atherothrombosis exposes vascular components to blood. Currently, new antithrombotic therapies are emerging. Herein we investigated thrombogenesis of human arteries with/without atherosclerosis, and the interaction of coagulation and vascular components, we and explored the anti-thrombogenic efficacy of blockade of the P2X purinoceptor 7 (P2X7). A confocal blood flow videomicroscopy system was performed on cryosections of internal mammary artery (IMA) or carotid plaque (CPL) determining/localizing platelets and fibrin. Blood from healthy donors elicited thrombi over arterial layers. Confocal microscopy associated thrombus with tissue presence of collagen type I, laminin, fibrin(ogen) and tissue factor (TF). The addition of antibodies blocking TF (aTF) or factor XI (aFXI) to blood significantly reduced fibrin deposition, variable platelet aggregation and aTF + aFXI almost abolished thrombus formation, showing synergy between coagulation pathways. A scarce effect of aTF over sub-endothelial regions, more abundant in tissue TF and bundles of laminin and collagen type I than deep intima, may suggest tissue thrombogenicity as molecular structure-related. Consistently with TF-related vascular function and expression of P2X7, the sections from CPL but not IMA tissue cultures pre-treated with the P2X7 antagonist A740003 demonstrated poor thrombogenesis in flow experiments. These data hint to local targeting studies on P2X7 modulation for atherothrombosis prevention/therapy.     

文献搜索引擎中特征项及权重的应用

针对目前用户在使用搜索引擎过程中,检索结果冗余、效率低下等问题,本文在对文献垂直搜索系统中Lucene.Net的索引算法研究基础上,结合用户实际专业检索需求,改变其算法中的激励因子,实验结果证明该方法确能提高搜索结果的相关度.     

基于微博扩展的用户兴趣主题挖掘算法

为解决基于微博的用户兴趣建模存在建模不全面的问题,提出一种基于微博扩展的用户兴趣主题挖掘算法。通过结合用户自身兴趣及用户关注人的兴趣来扩充微博用户兴趣,将兴趣分为长期兴趣、过期兴趣、近期兴趣,利用改进的TF． IDF算法分析相关微博内容,利用基于时间及文档频率加权的主题词重要度计算兴趣主题词得分,得出综合全面的用户兴趣。实验结果表明,综合分析用户及用户关注人的兴趣,可以有效得到真实、全面、包含潜在兴趣的用户个人兴趣。     

基于二次TF* IDF的互信息文本特征选择算法研究

在分析传统互信息法缺陷的基础上,提出一种基于二次TF*IDF的互信息特征选择算法,对仅在一个类别中出现的特征词的重要程度给予再次的衡量,解决了互信息值相等而无法进行有效特征选择的问题。利用贝叶斯分类器对该方法进行验证的结果表明该算法在文本分类效率和正确率上比原有方法有一定的提高。     

VPN及其安全技术研究

VPN(虚拟专用网)是在公共网的基础上建立的适合用户需求的虚拟网络，它可以节省费用，同时又具有高度安全性。介绍了VPN的相关技术，尤其是隧道技术，并分析了基于各层协议(第2层隧道协议PPTP／L2TF和第3层隧道协议IPSec)建立隧道的过程、方法和优缺点。     

Network Sorting Algorithm of Multi-Frequency Signal with Adaptive SNR

An signal noise ratio (SNR) adaptive sorting algorithm using the time-frequency (TF) sparsity of frequency-hopping (FH) signal is proposed in this paper.Firstly,the Gabor transformation is used as TF transformation in the system and a sorting model is established under undetermined condition;then the SNR adaptive pivot threshold setting method is used to find the TF single source.The mixed matrix is estimated according to the TF matrix of single source.Lastly,signal sorting is realized through improved subspace projection combined with relative power deviation of source.Theoretical analysis and simulation results show that this algorithm has good effectiveness and performance.     

Synthesis and Evaluation of Glycoconjugates Comprising N‐Acyl‐Modified Thomsen–Friedenreich Antigens as Anticancer Vaccines

Thomsen–Friedenreich (TF) antigen is an important tumor‐associated carbohydrate antigen. Its low immunogenicity, however, limits its application in the development of anticancer vaccines. To solve this problem, several N‐acyl‐modified TF derivatives were synthesized and conjugated with carrier protein CRM197 (a mutated diphtheria toxoid cross‐reactive material). The immunological results in BALB/c mice demonstrated that these modified TF antigen conjugates could stimulate the production of higher titers of IgG antibodies that cross‐reacted with native TF antigen. These glycoconjugates showed strong lymphocyte proliferative response, suggesting that they can induce cellular immunity. Furthermore, the elicited antisera reacted strongly with TF‐positive tumor cells (4T1). In particular, the N‐monofluoroacetyl‐modified TF conjugate 4 ‐CRM197 showed the strongest complement‐dependent cytotoxicity effect against 4T1 cells, implying the potential of this glycoconjugate as an anticancer vaccine.     

可行三维航迹个体的生成算法

在遗传算法中,初始种群中个体的质量对遗传算法的收敛速度影响较大.本文在分析航迹规划约束条件的基础上,设计了一种初始航迹个体的生成算法,产生的三维航迹个体满足飞行器性能约束,并能避免撞山危险,比传统的随机生成算法产生的航迹个体具有较大的适应度.使遗传算法能有效避免冗余搜索,从而提高航迹规划的收敛速度.     

动静态监测光纤光栅传感信号的实验研究

基于可调谐光纤TF Fabry-Pent（FP）滤波扫描传感光纤Bragg光栅（FBG）反射峰的原理．提出一种新颖的探测方法,分别从动静态两方面监测传感FBG反射谱与TF F-P透射谱的合成谱。实验中．通过加载到TF F-P滤波器的驱动信号的不同,在静态监测下,模拟并分析了光信号功率与转化电压的趋势图以及多次实验中探测到最大信号时两者的关系;用双踪示波器动态实时监测并分析了经光电探测（PD）及其放大调理电路转换后的电压变化信号。实验结果表明．PD探测的最大值与传感FBG反射峰对应。经过标定后的系统分辨率可达1pm,测量精度为0．01nm。