Photoregulation of Gene Expression with Ligand-Modified Caged siRNAs through Host/Guest Interaction

Small interfering RNA (siRNA) can effectively silence target genes through Argonate 2 (Ago2)-induced RNA interference (RNAi). It is very important to control siRNA activity in both spatial and temporal modes. Among different masking strategies, photocaging can be used to regulate gene expression through light irradiation with spatiotemporal and dose-dependent resolution. Many different caging strategies and caging groups have been reported for light-activated siRNA gene silencing. Herein, we describe a novel caging strategy that increases the blocking effect of RISC complex formation/process through host/guest (including ligand/receptor) interactions, thereby enhancing the inhibition of caged siRNA activity until light activation. This strategy can be used as a general approach to design caged siRNAs for the photomodulation of gene silencing of exogenous and endogenous genes.     

Identification of Host Cellular Protein Substrates of SARS-COV-2 Main Protease

The novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of coronavirus disease-19 (COVID-19) being associated with severe pneumonia. Like with other viruses, the interaction of SARS-CoV-2 with host cell proteins is necessary for successful replication, and cleavage of cellular targets by the viral protease also may contribute to the pathogenesis, but knowledge about the human proteins that are processed by the main protease (3CLpro) of SARS-CoV-2 is still limited. We tested the prediction potentials of two different in silico methods for the identification of SARS-CoV-2 3CLpro cleavage sites in human proteins. Short stretches of homologous host-pathogen protein sequences (SSHHPS) that are present in SARS-CoV-2 polyprotein and human proteins were identified using BLAST analysis, and the NetCorona 1.0 webserver was used to successfully predict cleavage sites, although this method was primarily developed for SARS-CoV. Human C-terminal-binding protein 1 (CTBP1) was found to be cleaved in vitro by SARS-CoV-2 3CLpro, the existence of the cleavage site was proved experimentally by using a His₆-MBP-mEYFP recombinant substrate containing the predicted target sequence. Our results highlight both potentials and limitations of the tested algorithms. The identification of candidate host substrates of 3CLpro may help better develop an understanding of the molecular mechanisms behind the replication and pathogenesis of SARS-CoV-2.     

CN decoration of dibenzofuran modified biphenyl for high triplet energy host for blue phosphorescent organic light-emitting diodes

A strongly electron deficient and high triplet energy host for blue emitters was developed by decorating a dibenzofuran modified biphenyl backbone structure with multiple CN units. Two hosts, 6,6′-bis(6-cyanodibenzo[b,d]furan-4-yl)-[1,1′-biphenyl]-3,3′-dicarbonitrile(CNDBF1) and 2,2′-bis(6-cyanodibenzo[b,d]furan-4-yl)-[1,1′-biphenyl]-4,4′-dicarbonitrile(CNDBF2), were derived from the CN decoration strategy for application in blue organic light-emitting diodes requiring high triplet energy host. They showed high triplet energy above 2.79 eV and acted as the electron transport type host based on the strong electron deficiency. The mixture of the CNDBF1 and CNDBF2 hosts with a hole transport type 3,3′-di(9H-carbazol-9-yl)-1,1′-biphenyl host performed as the exciplex host of a blue phosphor and accomplished high external quantum efficiency of 22.7% in the blue phosphorescent organic light-emitting diodes.     

Adsp-21060的主机接口在实时图像处理中的应用

简要介绍了数字信号处理器Adsp 2 10 6 0和大规模可编程逻辑器件EP1K5 0。详细讨论了Adsp 2 10 6 0的主机接口工作模式以及一个实时图像跟踪处理器的硬件组成原理 ,在这个系统中EP1K5 0充当了主机。调试结果表明 ,所提出的硬件结构设计思想工作效率高 ,完全能够胜任实时图像处理的实际需要。     

几种涉密网主机系统保护及审计技术的比较

论文比较详细地阐述了涉密网主机系统所面临的安全隐患,提出了涉密网主机安全防护所要解决的几个技术问题,并就可能实现的技术方法进行了探讨和比较。     

采用8031作主控器的时域FIR数字滤波器

长期以来，ＦＩＲ数字滤波器大多是在频域上实现。因为，在时域上实现ＦＩＲ数字滤波所遇到的首要问题，是输入信号序列与冲激响应序列的卷积运算速度难以提高。然而，随着超大规模集成电路的飞速发展，硬件集成度与运算速度获得极大的改观，在时域上实现ＦＩＲ数字滤波已成为可能。ＩＭＳＡ１００是高速、高精度３２级数字信号处理器，是完成卷积运算的理想器件。本文论证了用ＩＭＳＡ１００实现时域ＦＩＲ数字滤波器的可行性和硬件设计中的一些问题，并给出了应用举例。在设计中选用８０３１单片机做主控器，大大提高了性能价格比，使这一设计具有很高的实用价值。     

超分子主体化合物在分析化学中的应用研究进展

综述了近年来超分子主体化合物在分析化学领域的应用研究与进展。分别以冠醚、环糊精、杯芳烃、卟啉4类超分子为主体功能化合物的研究进行了详细的评述，并对这几类主体化合物在分析化学中的应用研究进行了展望。     

ZnS掺杂技术研究及应用现状

对现阶段国内外在ZnS及ZnS基材料掺杂改性方面的研究成果作一概述性介绍，综述了纳米级ZnS发光材料掺杂改性的基本机理，主要的掺杂技术、方法，并对多种掺杂元素的效果进行了分析比较。     

微机在应变测量中的应用

微机在应变测量中的应用，采用上位机和下位机组合方式．上位机为一台ＩＢＭ－ＰＣ／ＸＴ微机，用于对数据进行后处理．下位机以Ｚ８０－ＣＰＵ为控制器，由传感器、转换器、接口及外设等组成数据采集器，实施对应变、压力和位移等参数的采集、显示和打印（即数据的预处理）．上位机和下位机之间的数据传输，采用串行的方式，通过ＲＳ－２３２接口完成．本文主要以应变的采集处理为例，介绍系统功能、硬件配置及软件设计．     

~(133)Xe吸入法测量局部脑血流及其计算机在线分析

本文介绍了用吸入~(133)Xe的清除技术测定大脑血流量的原理和方法。讨论子数据获取及其计算机在线分析。报道了一些临床测定结果。