A Novel Artificially Humanized Anti-Cripto-1 Antibody Suppressing Cancer Cell Growth

Cripto-1 is a member of the EGF-CFC/FRL1/Cryptic family and is involved in embryonic development and carcinogenesis. We designed a novel anti-Cripto-1 artificial antibody and assessed the recognition to the antigen and the potential to suppress the growth of cancer stem cells. First, single chain antibody clones were isolated by bio-panning with the affinity to recombinant Cripto-1 protein from our original phage-display library. Then, the variable regions of heavy chain VH and light chain VL in each clone were fused to constant regions of heavy chain CH and light chain CL regions respectively. These fused genes were expressed in ExpiCHO-S cells to produce artificial humanized antibodies against Cripto-1. After evaluation of the expression levels, one clone was selected and the anti-Cripto-1 antibody was produced and purified. The purified antibody showed affinity to recombinant Cripto-1 at 1.1 pmol and immunoreactivity to cancer tissues and cell lines. The antibody was available to detect the immunoreactivity in tissue microarrays of malignant tumors as well as in Cripto-1 overexpressing cells. Simultaneously, the antibody exhibited the potential to suppress the growth of human colon cancer derived GEO cells overexpressing Cripto-1 with IC₅₀ at approximately 110 nM. The artificially humanized antibody is proposed to be a good candidate to target cancer cells overexpressing Cripto-1.     

网络学术资源网站的研究与实现

互联网知识庞杂,进行必要的学术资源导航必不可少。文章对目前国内外网络学术资源导航站点名称、网址和基本情况以表格形式进行说明,分别从人性化设计、资源内容、资源信息三方面对其进行研究,予以评价分析,指出各自特点和存在不足之处,最后推出新的网络学术资源导航站点建设思路和实践。     

浅谈让产品设计返璞归真

现代社会,人们对产品的要求不在是满足物美价廉,经久耐用。而是在审视产品在人的消费过程中给人带来的心灵上的满足。设计师面对这样的问题要如何设计出满足人心的产品呢?所以设计师就要重新审视设计的目的,让设计返璞归真。     

数字化背景下城市公共设施的人性化设计

目的研究城市公共设施在数字化背景下的人性化设计。方法从公共设施中人性化设计原则入手,针对大众的差异性,明确了城市公共设施中人性化设计的重点。通过对城市公共设施中的交通设施、信息设施、休闲设施、服务设施的应用案例进行分类研究,展现了在数字化背景下的城市公共设施中,人性化设计的具体运用。结论人性化设计是城市公共设施设计未来发展的必然趋势。数字化的运用,提升了城市公共设施原有的功能,使其更加实用化、时代化、艺术化与多样化。     

构建人性化的步行空间——重庆城市建设的可持续发展设计

20世纪60年代关注步行道设计的开端是源自于对于城市规划的反思,城市污染严重、环境不断遭受挑战,设计可持续发展的城市环境已经迫在眉睫,步行道是人类最为本能的行为之一,城市步行道的建立要从生理与心理两方面入手,山地城市步行道建设要注重人文关怀,重庆的山地城市风貌有利于建立立体化的步行道,使步行道成为山地城市景观的重要部分,关注重庆的步行道设计使城市充满活力,重庆的未来将是活力之都、宜居之城。     

城市养老院建筑的人性化设计

老年人是一群比较特殊的群体，城市疗养院要想为老年人提供更好的生活，在建筑设计方面应该尽可能地为老人提供方便，对老人衰退的感觉机能和生理机能加以综合考虑，从而实现最为人性化的设计，为老年群体的起居生活提供便利。     

交通设施的人性化设计分析

目前，各行各业都普遍运用到了人性化理念。本文主要对我国交通设施的人性化设计进行了分析与研究，同时针对一些问题总结出了相应的处理措施。     

Expression of Human DNAJ (Heat Shock Protein-40) B3 in Humanized UDP-glucuronosyltransferase 1 Mice

The human DNAJB3 gene encodes a DNAJ (Heat shock protein 40; Hsp40) homolog, subfamily B, member 3 chaperone protein (DNAJB3), which can be down-regulated in disease conditions, as observed in decreased expression of DNAJB3 mRNA in peripheral blood mononuclear cells (PBMC) of obese patients. Recently, humanized UDP-glucuronosyltransferase (UGT) 1 mice (hUGT1 mice) were developed, in which the introduced human UGT1 gene contained a gene encoding human DNAJB3. In the present study, we analyzed the expression of human DNAJB3 mRNA in hUGT1 mice. Among the examined tissues, the testis had the highest expression of human DNAJB3 mRNA, while the lowest expression was observed in the liver. We found that the pattern of tissue-specific expression of mouse Dnajb3 in hUGT1 mice was very similar to that of human DNAJB3. We further demonstrated that the expression of human DNAJB3 in the liver was significantly reduced in high-fat-diet-fed hUGT1 mice compared to the expression level in the control mice, indicating that the expression of human DNAJB3 in hUGT1 mice could be similarly regulated in disease conditions such as obesity. Humanized UGT1 mice might therefore be useful to investigate the physiological role of human DNAJB3 in vivo.     

人性化的城市交通空间规划设计实践

长期以来,我国城市交通围绕小汽车为核心发展,对行人、自行车、公共交通等方面的关注不够,交通系统的人性化亟需完善和提升。尽管近年来随着绿色交通的兴起,交通系统的人性化逐渐被重视和关注,但人性化的交通体系规划建设仍然面临很大的挑战。本文从交通基础设施建设、交通使用者、城市管理者三个方面分析了人性化交通系统发展面临的困境,从宏观、中观、微观三个层面提出了构建人性化交通空间的对策和建议。