Cell-Free Synthesis of Selenoproteins in High Yield and Purity for Selective Protein Tagging

The selenol group of selenocysteine is much more nucleophilic than the thiol group of cysteine. Selenocysteine residues in proteins thus offer reactive points for rapid post-translational modification. Herein, we show that selenoproteins can be expressed in high yield and purity by cell-free protein synthesis by global substitution of cysteine by selenocysteine. Complete alkylation of solvent-exposed selenocysteine residues was achieved in 10 minutes with 4-chloromethylene dipicolinic acid (4Cl-MDPA) under conditions that left cysteine residues unchanged even after overnight incubation. Gd^III−Gd^III distances measured by double electron–electron resonance (DEER) experiments of maltose binding protein (MBP) containing two selenocysteine residues tagged with 4Cl-MDPA-Gd^III were indistinguishable from Gd^III−Gd^III distances measured of MBP containing cysteine reacted with 4Br-MDPA tags.     

世界各国(或地区)推进RFID的策略

介绍了一些国家和地区发展RFID的政策和推进措施，总结了各国发展RFID的共性。     

Depth and temperature selection of lake charr (Salvelinus namaycush) ecotypes in Lake Superior revealed by popup satellite archival tags

Lake charr exhibit morphological diversity in large North American lakes, largely attributed to habitat partitioning. Bathythermal habitats of lean lake charr have been assessed but remain largely unknown for other lake charr ecotypes. Popup satellite archival tags (PSATs) were used to determine depth and temperature profiles of lean (n = 15), siscowet (n = 16) and redfin (n = 3) lake charr ecotypes in Lake Superior during segments of the year. Monthly median depths of leans were < 20 m throughout the year while the median depth of redfins was 24–32 m (June-September). Monthly median depth of siscowets ranged from 103 to 204 m but they exhibited four distinctive patterns: deep (>80 m), shallow (<10 m), extreme vertical movements (>80–0 m), and high frequency vertical movements between 100 and 125 m. Siscowets were the most stenothermal with habitat temperatures generally 4–5 °C but not exceeding 12 °C. Leans were found as low as 0 °C (January-March) and up to 15 °C (July-September). Median monthly habitat temperatures for redfins were 1–3 °C colder than leans during July-September. PSATs confirmed that siscowets exhibit extreme vertical movements and may feed near the surface. The timing and duration of the extreme vertical movements exhibited by siscowets did not appear to be associated with time of day (i.e., not diel vertical movements) and may be described as opportunistic movements presumably related to foraging. Data obtained from the PSATs reinforces the view that lake charr are highly adaptive and can thrive in cold, oligotrophic lakes by optimizing the use of the entire water column.     

基于物联网技术的智慧种业全周期追溯防伪管理系统

利用RFID技术、电子技术、计算机技术、数据库技术、3G/4G通讯技术、特种标签制造技术及3D仿真技术等,构建了智慧种业全周期追溯防伪管理系统,该系统在种子生产包装环节将电子玉米标签随种子混装于包装袋内,同时将品种、批次、生产日期等信息进行赋码,充分利用超高频远距离批量识别特性,实现批量出入库、物流各环节的全程监控,利用非可视、不接触识读特性实现终端防伪查询及隐蔽式窜货稽查,很好地解决了目前种子行业防伪、防窜货手段所存在的易仿造、易破坏的问题,为国家种业安全提供一个新型的防伪、防窜货技术平台。     

基于Tiff图像数字化档案的研究

Tiff文件格式是一个丰富的图像格式,可以支持多种图像,现在已经成为存储档案数字化图像的标准格式.现有的应用程序都可以很好的支持Tiff图像格式.本文从Tiff的结构、特性、分类等几个方面,对Tiff文件进行了详细的分析.在支持传真的Class-F分类基础上,通过配置Tiff标签和在图像数据区域附加图像描述信息,对基于Tiff图像的数字化档案进行研究,并把此研究应用于焊接培训中,并取得了预期的效果.     

应用射频技术的商场智能导购系统

设计的电子导购系统,包括电子标签的选择,嵌入式 Android 系统的选择,电子导购系统功能模块的组成。同时设计了客户端的两大功能模块,包括促销信息功能模块的设计,电子地图定位模块的设计。讨论了定位RFID 标签的布局,并利用 RSSI 来评估其接收的信号强度并计算出标签和读写器的距离,根据相邻的四个标签的坐标,采用极大似然估计方法获得智能购物车的位置,能够用于商场内精准的定位。     

基于几何分布的RFID标签数目估算方法

已有的标签数目估算方法准确率低、估算范围小,且标签数目增加时,时隙数目须成比例增加,否则估算误差率将大幅提高。针对上述问题,提出一种基于几何分布的无线射频识别(RFID)标签数目估算方法。在估算标签数目时,将帧长设定为固定值,并在标签中采用多个哈希函数,以减少几何分布偏差,降低估算误差率。实验结果表明,在时间复杂度、空间复杂度不高于其他算法的前提下,该方法能降低估算误差率。     

基于分段搜索的多RFID标签抗冲突方法*

由于二元树抗冲突方法消耗的识别时间随标签数量呈线性增长,因而提高巨量（>100）标签的抗冲突识别的效率对于推动RFID的应用显得尤为重要。对此本文提出一种分段搜索清点RFID标签的抗冲突方法,通过减少清点过程中标签对阅读器回传数据的位数,达到减少传输耗时的目的。将其与现有的动态二进制搜索法对比,仿真结果表明,当标签突破一定数量,对于特定的分段方式,该算法能够显著降低清点过程的时间消耗。     

用户权限控制在SSMBSS中的设计与实现

在Web开发中,权限往往是一个极其复杂的问题,针对不同的应用,需要根据项目的实际情况和具体架构,在维护性、灵活性、完整性等多个方面比较、权衡。本文简要介绍了在Struts和Hibernate框架下基于角色的权限管理（KBAC）思想．提出了自定义标签和Struts标签相结合的方式,提高了系统的灵活性和安全性,达到MVC很好的分离。     

工控图形的组态优化设计

随着工程数量的增加,系统组态成本加大显得十分突出.针对DCS图形组态的耗时费力,提出了优化解决方案.标签替换辅助工具软件综合多种技术,简化了组态过程.用图形模板简化图形操作;用每个标签代替复杂信息语句;用电子表格汇总登记指定信息.用软件对图形文本的XML语言进行编辑和信息替换,自动批量处理图形.所有替换数据信息被定义在开放的配置文本文件中,方便其修改和扩展.设计思想可作为组态优化的参考模式.