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Dovile Stravinskiene Aiste Sliziene Lina Baranauskiene Vilma Petrikaite Aurelija Zvirbliene 《International journal of molecular sciences》2020,21(24)
Monoclonal and recombinant antibodies are widely used for the diagnostics and therapy of cancer. They are generated to interact with cell surface proteins which are usually involved in the development and progression of cancer. Carbonic anhydrase XII (CA XII) contributes to the survival of tumors under hypoxic conditions thus is considered a candidate target for antibody-based therapy. In this study, we have generated a novel collection of monoclonal antibodies (MAbs) against the recombinant extracellular domain of CA XII produced in HEK-293 cells. Eighteen out of 24 MAbs were reactive with cellular CA XII on the surface of live kidney and lung cancer cells as determined by flow cytometry. One MAb 14D6 also inhibited the enzymatic activity of recombinant CA XII as measured by the stopped-flow assay. MAb 14D6 showed the migrastatic effect on human lung carcinoma A549 and renal carcinoma A498 cell lines in a ‘wound healing’ assay. It did not reduce the growth of multicellular lung and renal cancer spheroids but reduced the cell viability by the ATP Bioluminescence assay. Epitope mapping revealed the surface-exposed amino acid sequence (35-FGPDGENS-42) close to the catalytic center of CA XII recognized by the MAb 14D6. The variable regions of the heavy and light chains of MAb 14D6 were sequenced and their complementarity-determining regions were defined. The obtained variable sequences were used to generate recombinant antibodies in two formats: single-chain fragment variable (scFv) expressed in E. coli and scFv fused to human IgG1 Fc fragment (scFv-Fc) expressed in Chinese Hamster Ovary (CHO) cells. Both recombinant antibodies maintained the same specificity for CA XII as the parental MAb 14D6. The novel antibodies may represent promising tools for CA XII-related cancer research and immunotherapy. 相似文献
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钨合金圆筒爆破试验破片分布的统计分析 总被引:1,自引:1,他引:0
采用圆筒爆破试验方法研究了易碎型钨合金的破片分布特征,并与93W合金的结果进行了对比.对破片的数量和质量分布特征进行了统计分析,发现易碎型钨合金的破片率明显高于93W合金,而且破片的均匀性也明显好于93W合金,但易碎钨合金的破片回收率低于93W合金,而且受装药量的影响较大. 相似文献
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Christos Bouras Agisilaos Konidaris 《International Journal of Communication Systems》2005,18(2):119-142
Redundant data transfers over the Web, can be mainly attributed to the repeated transfers of unchanged data. Web caches and Web proxies are some of the solutions that have been proposed, to deal with the issue of redundant data transfers. In this paper we focus on the efficient estimation and reduction of redundant data transfers over the Web. We first prove that a vast amount of redundant data is transferred in Web pages that are considered to carry fresh data. We show this by following an approach based on Web page fragmentation and manipulation. Web pages are broken down to fragments, based on specific criteria. We then deal with these fragments as independent constructors of the Web page and study their change patterns independently and in the context of the whole Web page. After the fragmentation process, we propose solutions for dealing with redundant data transfers. This paper has been based on our previous work on ‘Web Components’ but also on related work by other researchers. It utilises a proxy based, client/server architecture, and imposes changes to the algorithms executed on the Proxy server and on clients. We show that our proposed solution can considerably reduce the amount of redundant data transferred on the Web. Copyright © 2004 John Wiley & Sons, Ltd. 相似文献
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大型储罐底板焊接及变形控制是保证储罐整体施工质量的关键环节,采用碎丝填充的焊接方法可以防止变形,同时可以有效地避免应力集中,提高施工质量。文章介绍了碎丝埋弧焊的原理、工艺参数和焊接方法。实践证明,填充碎丝不仅是一种减小底板焊接变形的手段,同时可以充分利用焊接热能,节约焊接时间和焊剂用量,经济效益好,是一种值得推广的技术。 相似文献
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文中主要针对多爆炸成型弹丸空爆战斗部,在分析区域识别和占空比识别两种不同扫描准则的基础上,应用蒙特卡洛方法计算了战斗部对目标的毁伤概率.通过仿真计算结果对比分析,获得了不同扫描准则对毁伤概率的影响,结果可为多爆炸成型弹丸空爆战斗部设计和选择使用扫描准则提供参考依据. 相似文献
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侧重研究了破片式战斗部装药外形的优化设计技术。提出了装药外形与破片飞散特性(如破片飞散角,分布带宽等)之间的数学描述;建立了圆弧和对数螺线装药外形母线与战斗部其他设计参数间的数学模型。在假定约束条件下,优化设计了破片了聚焦式战斗部的结构参数;静爆试验结果表明,破片分布与理论计算吻合较好。 相似文献
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Pharaoh Fellow Mwale Chi-Hsin Lee Peng-Nien Huang Sung-Nien Tseng Shin-Ru Shih Hsin-Yuan Huang Sy-Jye Leu Yun-Ju Huang Liao-Chun Chiang Yan-Chiao Mao Wei-Chu Wang Yi-Yuan Yang 《International journal of molecular sciences》2021,22(8)
Coxsackievirus A16 (CA16) is one of the major causative agents of hand, foot, and mouth disease (HFMD). Children aged <5 years are the most affected by CA16 HFMD globally. Although clinical symptoms of CA16 infections are usually mild, severe complications, such as aseptic meningitis or even death, have been recorded. Currently, no vaccine or antiviral therapy for CA16 infection exists. Single-chain variable fragment (scFv) antibodies significantly inhibit viral infection and could be a potential treatment for controlling the infection. In this study, scFv phage display libraries were constructed from splenocytes of a laying hen immunized with CA16-infected lysate. The pComb3X vector containing the scFv genes was introduced into ER2738 Escherichia coli and rescued by helper phages to express scFv molecules. After screening with five cycles of bio-panning, an effective scFv antibody showing favorable binding activity to proteins in CA16-infected lysate on ELISA plates was selected. Importantly, the selected scFv clone showed a neutralizing capability against the CA16 virus and cross-reacted with viral proteins in EV71-infected lysate. Intriguingly, polyclonal IgY antibody not only showed binding specificity against proteins in CA16-infected lysate but also showed significant neutralization activities. Nevertheless, IgY-binding protein did not cross-react with proteins in EV71-infected lysate. These results suggest that the IgY- and scFv-binding protein antibodies provide protection against CA16 viral infection in in vitro assays and may be potential candidates for treating CA16 infection in vulnerable young children. 相似文献