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| 黄单胞冰核活性细菌发酵制备的初步研究 | |
| 引用本文： | 胡卓炎,何松,孙福在,赵廷昌,钟士清.黄单胞冰核活性细菌发酵制备的初步研究[J].食品与发酵工业,2001,27(3):5-8. |
| 作者姓名： | 胡卓炎 何松 孙福在 赵廷昌 钟士清 |
| 作者单位： | 1. 华南农业大学食品科学系, 2. 中山出入境检验检疫局, 3. 中国农业科学研究院植物保护研究所, |
| 基金项目： | 国家自然科学基金项目(No.30070537)，广东省自然科学基金资助项目(No.970045) |
| 摘 要： | 应用正交设计与均匀设计对黄单胞冰核活性细菌的发酵制备条件进行了初步研究。结果表明 ,琥珀酸抑制了黄单胞菌生长与冰核活性的表达 ,甘油、蔗糖、乳糖、谷氨酸钠不同程度地促进了冰核活性和单胞菌的生长。较优培养基配方为酵母粉 1%、大豆蛋白胨 1%、MgSO4 ·7H2 O 0 0 5%、甘油 1%、蔗糖 2 %、乳糖 1%、谷氨酸钠 0 3% ,pH 7 0 ;较优发酵组合为细菌种龄 72h ,发酵时间 4 5h |
| 关 键 词： | 冰核细菌 冰核活性 发酵 |
| 修稿时间： | 2000年9月27日 |
Studies on Fermentation of Ice Nucleation-active Xanthomonas campestris IN-1 |
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| Hu Zhuoyan,HE Song,Sun Fuzai,Zhao Tingchang,Zhong Shiqing.Studies on Fermentation of Ice Nucleation-active Xanthomonas campestris IN-1[J].Food and Fermentation Industries,2001,27(3):5-8. | |
| Authors: | Hu Zhuoyan HE Song Sun Fuzai Zhao Tingchang Zhong Shiqing |
| Affiliation: | Hu Zhuoyan 1 He Song 2 Sun Fuzai 3 Zhao Tingchang 3 Zhong Shiqing 1 1 |
| Abstract: | The fermentation of ice nucleation-active bacteria (Xanthomonas campestris IN-1 )was studied in this paper. The results showed that succinate in the growth medium had the negative function to the ice nucleation activity of Xanthomonas campestris IN-1, which was not consistent to the literature. Glycerol, sucrose, lactose and sodium glutamate could promote the icenucleation activity and the cell growth. The optimum cultivation medium was presented as 1% of yeast extract, 1% of bacto-peptone, 0.05 % of magnesium sulfate, 1% of glycerol, 2 % of sucrose, 1% of lactose, 0.3 % of sodium glutamate, pH7.0. The optimum fermentation conditions were predicted as 72h of bacterial ages, 45h of fermentation time. |
| Keywords: | ice nucleation-active bacteria ice nucleation activity fermentation |
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