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| 洋葱皮中黄酮类物质的提取及分离纯化研究 | |
| 引用本文： | 张仁堂,秦静,孙欣,林云良. 洋葱皮中黄酮类物质的提取及分离纯化研究[J]. 中国食物与营养, 2012, 18(8): 50-54 |
| 作者姓名： | 张仁堂 秦静 孙欣 林云良 |
| 作者单位： | 1. 山东农业大学食品科学与工程学院,山东泰安,271018 2. 华中农业大学食品科学技术学院,武汉,430070 3. 山东省分析测试中心,济南,250014 |
| 基金项目： | 山东省高等学校科技计划项目(项目编号:J10LC14);泰安市大学生科技创新计划项目(项目编号:2009D1007) |
| 摘 要： | 采用超声波辅助法提取洋葱皮中黄酮类物质,通过柱层析等方法进行分离纯化,利用高效液相色谱对黄酮类物质进行检测,在超声波辅助萃取洋葱皮中总黄酮的研究中,确定乙醇浓度80%、料液比1∶10、超声时间20min条件下对总黄酮提取率最高。获得总黄酮物质后采用萃取、聚酰胺柱层析进行分离纯化,获得不同浓度乙醇洗脱组分,建立HPLC方法为进样量5μL、检测波长360nm、甲醇∶水（含0.1%冰乙酸）=45∶55、柱温为室温。分离纯化中得到结晶,通过质谱、核磁共振鉴定此晶体物质为槲皮素。 |
| 关 键 词： | 洋葱皮 黄酮 聚酰胺柱层析 HPLC 槲皮素 |
Extraction,Separation and Purification of Flavones in Onionskin |
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| ZHANG Ren-tang,QIN Jing,SUN Xin,LIN Yun-liang. Extraction,Separation and Purification of Flavones in Onionskin[J]. Food and Nutrition in China, 2012, 18(8): 50-54 | |
| Authors: | ZHANG Ren-tang QIN Jing SUN Xin LIN Yun-liang |
| Affiliation: | 1College of Food Science and Engineering,Shandong Agricultural University,Tai’an 271018,China; 2College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China; 3Shandong Analysis and Test Center,Jinan 250014,China) |
| Abstract: | This paper studied the technological process of extracting flavones from onionskin,established a HPLC method for determining the flavones in the extraction of the onionskin.The optimum ultrasonic extraction conditions for flavones were also established which were soak onion for 20min with 80% ethanol concentration and ratio of liquid to solid was 1∶ 10.In the study of the HPLC method for determining the flavones,we got the condition of a 5μL sample injected,detection was carried out at 360 nm,a mixture of methanol and water with 0.1% glacial acetic acid(45∶ 55) was used as mobile phase.In the course of separation and purification,we got the crystallization with high pure.By the way of MS and NMR,we knew this material was quercetin. |
| Keywords: | onionskin flavones polyamine column chromatography HPLC quercetin |
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