充气糖果中胭脂红酸的高效液相色谱测定 |
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引用本文: | 张飞,郑家概,付强,刘军,李圣男,林钦恒. 充气糖果中胭脂红酸的高效液相色谱测定[J]. 广州化工, 2013, 0(24): 110-111,134 |
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作者姓名: | 张飞 郑家概 付强 刘军 李圣男 林钦恒 |
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作者单位: | 中国广州分析测试中心,广东广州510070 |
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摘 要: | 建立充气糖果中胭脂红酸的高效液相色谱方法。糖果经乙醇/氨杉水超声提取,水浴蒸至近干,氨水溶液定容。采用C18柱(4.6mm×250mm,5μm),流动相为0.01%磷酸溶液和甲醇/乙腈,梯度洗脱,流速1.0mL/min,检测波长494nm。胭脂红酸在2.5—40.0mg/L线性良好,回收率为87%~102%,RSD为1.75%,检出限为0.3mg/kg。该方法操作简便、准确,适于充气糖果中胭脂红酸的测定。
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关 键 词: | 胭脂红酸 充气糖果 高效液相色谱法 |
Determination of Carminic Acid in Aerated Confections by High Performance Liquid Chromatography |
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Affiliation: | ZHANG Fei, ZHENG Jia - gai, FU Qiang, LIU Jun, LI Sheng - nan, LIN Qin - heng ( China National Analytical Center ( Guangzhou), Guangdong Guangzhou 510070, China) |
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Abstract: | A method for determination of carminic acid in aerated confections by high performance liquid chromatography (HPLC) was developed. The aerated confections were extracted by ultrasonic technique with ethanol/ ammonia/water. The extract evaporated nearly to dryness on water bath then volumed it by ammonia. HPLC separation of carminic acid was conducted on a C18 column (4. 6 mm ×250 mm, 5 μm) with a mobile phase of 0. 01% phosphoric acid and methanol/acetonitrile under a gradient elution condition at a flow rate of 1.0 mL/min and the detection was carried out at a wavelength of 494 nm. The linearity was obtained in the range of 2. 5 ~ 40. 0 mg/L. The average recoveries of samples were in the range of 87 - 102% and RSD was 1.75%. The determination of limit was 0.3 mg/kg. This method was found to be fast, reliable and good linearity with low relative standard deviation, which can be used for determination of earminic acid in aerated confections. |
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Keywords: | carminic acid aerated confections HPLC |
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