Crosslinked fibroin nanoparticles using EDC or PEI for drug delivery: physicochemical properties,crystallinity and structure |
| |
Authors: | " target="_blank">Duy Toan Pham " target="_blank">Nuttawut Saelim Waree Tiyaboonchai |
| |
Affiliation: | 1.Faculty of Pharmaceutical Sciences,Naresuan University,Phitsanulok,Thailand;2.The Center of Excellence for Innovation in Chemistry (PERCH-CIC), Commission on Higher Education,Ministry of Education,Bangkok,Thailand;3.The Center of Excellence in Medical Biotechnology,Naresuan University,Phitsanulok,Thailand |
| |
Abstract: | A new silk fibroin-based nanoparticles (FNPs) have been successfully developed utilizing crosslinker EDC (EDC-FNPs) or cationic polymer poly(ethyleneimine) (PEI-FNPs). All developed crosslinked FNPs show a similar spherical particle size of ~?300 nm. Depending on the amount of EDC or the addition of PEI, the zeta potential could be controlled favorably and range from ??15 mV to +?30 mV. Particle structures and crystallinity index are determined and compared using different techniques; X-ray diffraction (XRD), Fourier-transformed infrared spectroscopy (FT-IR), differential scanning calorimetry and nuclear magnetic resonance (NMR). The most reliable crystallinity calculation methods are based on FT-IR and NMR. The degree of fibroin crystallinity increases with increasing EDC content, whereas PEI reduces it. Exceptionally, XRD shows a reverse order of crystallinity due to the inability to detect short-range ordered structures. Proposed schematic particle structures match all experimental data. In conclusion, the crosslinked fibroin-based nanoparticle properties could be manipulated by using carbodiimide or PEI crosslinkers. In addition, both EDC-FNPs and PEI-FNPs showed high potential as a drug delivery system. |
| |
Keywords: | |
本文献已被 SpringerLink 等数据库收录! |
|