等温扩增技术在食源性致病菌检测中的研究进展

食源性致病菌污染是影响食品质量安全的重要因素之一,加强食源性致病菌的检测力度对于保证食品安全以及预防食源性疾病至关重要。基于核酸的分子检测技术较传统的培养方法相比,更加快速、灵敏、高效。等温扩增技术以反应条件简单等优点逐渐替代变温扩增技术。该文简述了环介导等温扩增、链替代等温扩增、单引物等温扩增、滚环等温扩增以及跨越式滚环等温扩增5种技术的研究进展及其在食源性致病菌快速检测方面的应用,指出了等温扩增反应存在的共性问题,并提出解决措施。此外,还对这5种等温扩增技术进行比较分析,为食源性致病菌的现场快速检测与筛查提供参考依据。

Research progress on isothermal amplification technology in the detection of foodborne pathogens

Contamination of foodborne pathogens is one of the important factors affecting food quality and safety.Therefore,strengthening the monitoring of foodborne pathogens is essential to ensure food safety and prevent foodborne diseases.Compared with traditional culture methods,nucleic acid-based molecular detection technology is faster,more sensitive,and more efficient.Isothermal amplification technologies gradually replace variable temperature amplification technologies with the advantages of simple reaction conditions.This paper briefly described the research progress on loop-mediated isothermal amplification,strand displacement amplification,single primer isothermal amplification,rolling circle amplification,and saltatory rolling circle amplification,as well as their applications in rapid detection of foodborne pathogenic bacteria.In addition,this research pointed out the common problems existing in the isothermal amplification reaction,and proposed solutions.Otherwise,the five isothermal amplification techniques were compared,which provide a reference for the rapid on-site detection and screening of foodborne pathogens.