Viability of Two Freeze-dried Strains of Bifidobacterium and of Commercial Preparations at Various Temperatures During Prolonged Storage |
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Authors: | FA Bruno NP Shah |
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Affiliation: | Authors Bruno and Shah are with the School of Molecular Sciences, Victoria Univ., Werribee Campus, PO Box 14428, Melbourne City Mail Centre, Victoria 8001, Australia. |
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Abstract: | ABSTRACT: Viability of bifidobacteria in freeze-dried probiotic products at various temperatures during prolonged storage was assessed. Bifidobacterium longum 1941 and B. longum 536 were freeze-dried and capsules were manufactured. Five commercial probiotic capsule products were also tested. The capsules were stored at -18 °C, 4 °C, and 20 °C. Cell counts were enumerated using MRS-NNLP agar at 37 °C for 72 h under anaerobic conditions at 0, 1, 2, 5, and 8 mo (commercial capsules) and at 0, 1, 2, 5, 8, 11, 14, 17, and 20 mo (laboratory capsules). Storage at 20 °C showed the greatest decline in the viability of bifidobacteria, whereas that at -18 °C showed the least decrease. |
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Keywords: | probiotics viability storage temperature freeze-drying |
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