Immunostimulatory Potential of Extracellular Vesicles Isolated from an Edible Plant,Petasites japonicus,via the Induction of Murine Dendritic Cell Maturation |
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Authors: | Jeong Moo Han Ha-Yeon Song Seung-Taik Lim Kwang-Il Kim Ho Seong Seo Eui-Baek Byun |
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Affiliation: | 1.Advanced Radiation Technology Institute, Korea Atomic Energy Research Institute, Jeongeup 56212, Korea; (J.M.H.); (H.-Y.S.); (K.-I.K.); (H.S.S.);2.Department of Biotechnology, College of Life Science and Biotechnology, Korea University, Seoul 02841, Korea;3.Department of Radiation Science and Technology, University of Science and Technology, Daejeon 34113, Korea |
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Abstract: | Extracellular vesicles (EVs) have recently been isolated from different plants. Plant-derived EVs have been proposed as potent therapeutics and drug-delivery nanoplatforms for delivering biomolecules, including proteins, RNAs, DNAs, and lipids. Herein, Petasites japonicus-derived EVs (PJ-EVs) were isolated through a series of centrifugation steps and characterized using dynamic light scattering and transmission electron microscopy. Immunomodulatory effects of PJ-EVs were assessed using dendritic cells (DCs). PJ-EVs exhibited a spherical morphology with an average size of 122.6 nm. They induced the maturation of DCs via an increase in the expression of surface molecules (CD80, CD86, MHC-I, and MHC-II), production of Th1-polarizing cytokines (TNF-α and IL-12p70), and antigen-presenting ability; however, they reduced the antigen-uptake ability. Furthermore, maturation of DCs induced by PJ-EVs was dependent on the activation and phosphorylation of MAPK and NF-κB signal pathways. Notably, PJ-EV-treated DCs strongly induced the proliferation and differentiation of naïve T cells toward Th1-type T cells and cytotoxic CD8+ T cells along with robust secretion of IFN-γ and IL-2. In conclusion, our study indicates that PJ-EVs can be potent immunostimulatory candidates with an ability of strongly inducing the maturation of DCs. |
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Keywords: | extracellular vesicles dendritic cells immunostimulatory T cells Petasites japonicus |
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