山麻楂黄酮提取条件的响应面法优化及不同部位黄酮含量的测定

以连云港花果山野生山麻楂为原料，研究山麻楂黄酮的提取工艺。在单因素试验基础上，根据Box-Behnken试验设计原理，选取提取温度、提取时间、液料比三因素三水平进行响应面试验，建立黄酮提取率的二次回归方程，确定提取工艺的优化组合条件。结果表明：提取工艺最佳条件为提取温度65℃、提取时间112min、液料比27:1(mL/g)、提取2次。该条件下黄酮提取率预测值为2.652%，验证值为2.649%。对山麻楂不同部位黄酮含量测定的结果表明，山麻楂不同部位黄酮含量存在明显差异，叶中最高，为5.264%，花、茎次之，为2.983%和2.145%，根最低，为1.861%。

Optimization of Flavonoids Extraction from Gypsophila oldhamiana Miq. Using Response Surface Methodology and Determination of Flavonoids in Different Botanical Parts

Response surface methodology was employed to optimize the extraction of flavonoids from young leaves and stems of wild Gypsophila oldhamiana Miq. collected from Huaguo Mountain region in Lianyungang with the aim of developing a generalized approach for sample preparation for the determination of flavonoids in other botanical parts of this plant. A quadratic regression model that describes extraction efficiency as a function of temperature, time and solvent-to-solid ratio was established using a Box-Behnken experimental design. The optimum extraction conditions were obtained as follows: two extraction cycles at 65 ℃ for 112 min each cycle with a solvent-to-solid ratio of 27:1 (mL/g). Under these conditions, the predicted flavonoid yield was 2.652% as opposed to 2.649% of the actual value. There was a considerable difference in flavonoid content among different botanical parts of Gypsophila oldhamiana Miq.: 5.264%, 2.983%, 2.145% and 1.861% in leaves, flowers, stems and roots, respectively.