| 响应面法优化产赤藓糖醇菌株RH-UV-L4-F9的发酵条件 |
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| 引用本文： | 胡耀辉,李成斌,刘俊梅.响应面法优化产赤藓糖醇菌株RH-UV-L4-F9的发酵条件[J].食品科学,2012,33(23):171-174. |
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| 作者姓名： | 胡耀辉 李成斌 刘俊梅 |
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| 作者单位： | 吉林农业大学食品科学与工程学院 |
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| 基金项目： | 国家科技部成果转化基金项目(2010GB2B100108);吉林省教育厅[2011]第31号项目 |
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| 摘 要： | 为进一步提高糖蜜玉米浆发酵生产赤藓糖醇的产量，以高产赤藓糖醇耐高渗酵母RH-UV-L4-F9为出发菌株，采用响应面法优化发酵工艺参数。根据Box-Benhnken设计原理，在单因素试验基础上，采用三因素三水平响应面分析，确定最佳发酵工艺参数为：温度32.3℃、pH5.1、摇床转速176r/min。在此优化条件下赤藓糖醇产量达到166.89mg/mL，是初始条件下的1.2倍。
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| 关 键 词： | 赤藓糖醇 响应面分析 发酵 优化 |
| 收稿时间： | 2011-10-01 |
Optimization of Fermentation Conditions for Erythritol Production by Yeast RH-UV-L4-F9 Using Response Surface Methodology |
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| HU Yao-hui,LI Cheng-bin,LIU Jun-mei.Optimization of Fermentation Conditions for Erythritol Production by Yeast RH-UV-L4-F9 Using Response Surface Methodology[J].Food Science,2012,33(23):171-174. |
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| Authors: | HU Yao-hui LI Cheng-bin LIU Jun-mei |
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| Affiliation: | (College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China) |
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| Abstract: | This work reports the application of response surface methodology based on a three-variable, three-level Box-Benhnken design to optimize fermentation parameters to enhance the production of erythritol from molasses and corn syrup using yeast RH-UV-L4-F9, a potent erythritol producing strain. The optimal values of temperature, pH and shaking speed were 32.3 ℃, 5.1 and 176 r/min, respectively. Under the optimized conditions, the yield of erythritol was 166.89 mg/mL, which was 1.2-fold higher than before the optimization. |
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| Keywords: | erythritol response surface methodology fermentation optimization |
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