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响应面法优化产赤藓糖醇菌株RH-UV-L4-F9的发酵条件
引用本文:胡耀辉,李成斌,刘俊梅. 响应面法优化产赤藓糖醇菌株RH-UV-L4-F9的发酵条件[J]. 食品科学, 2012, 33(23): 171-174
作者姓名:胡耀辉  李成斌  刘俊梅
作者单位:吉林农业大学食品科学与工程学院
基金项目:国家科技部成果转化基金项目(2010GB2B100108);吉林省教育厅[2011]第31号项目
摘    要:为进一步提高糖蜜玉米浆发酵生产赤藓糖醇的产量,以高产赤藓糖醇耐高渗酵母RH-UV-L4-F9为出发菌株,采用响应面法优化发酵工艺参数。根据Box-Benhnken设计原理,在单因素试验基础上,采用三因素三水平响应面分析,确定最佳发酵工艺参数为:温度32.3℃、pH5.1、摇床转速176r/min。在此优化条件下赤藓糖醇产量达到166.89mg/mL,是初始条件下的1.2倍。

关 键 词:赤藓糖醇  响应面分析  发酵  优化  
收稿时间:2011-10-01

Optimization of Fermentation Conditions for Erythritol Production by Yeast RH-UV-L4-F9 Using Response Surface Methodology
HU Yao-hui,LI Cheng-bin,LIU Jun-mei. Optimization of Fermentation Conditions for Erythritol Production by Yeast RH-UV-L4-F9 Using Response Surface Methodology[J]. Food Science, 2012, 33(23): 171-174
Authors:HU Yao-hui  LI Cheng-bin  LIU Jun-mei
Affiliation:(College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China)
Abstract:This work reports the application of response surface methodology based on a three-variable, three-level Box-Benhnken design to optimize fermentation parameters to enhance the production of erythritol from molasses and corn syrup using yeast RH-UV-L4-F9, a potent erythritol producing strain. The optimal values of temperature, pH and shaking speed were 32.3 ℃, 5.1 and 176 r/min, respectively. Under the optimized conditions, the yield of erythritol was 166.89 mg/mL, which was 1.2-fold higher than before the optimization.
Keywords:erythritol  response surface methodology  fermentation  optimization  
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