| 免疫磁珠法检测脱水蒜制品中沙门氏菌 |
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| 引用本文： | 余晓峰,张萍,宗凯,严轶,孙娟娟,李云飞,郑海松,陈雪娇. 免疫磁珠法检测脱水蒜制品中沙门氏菌[J]. 食品科学, 2012, 33(24): 257-259. DOI: 10.7506/spkx1002-6630-201224054 |
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| 作者姓名： | 余晓峰 张萍 宗凯 严轶 孙娟娟 李云飞 郑海松 陈雪娇 |
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| 作者单位： | 1.安徽出入境检验检疫局技术中心，安徽 合肥 230022；2.食品安全分析检测安徽省重点实验室，安徽 合肥 230022 |
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| 基金项目： | 安徽出入境检验检疫局科研项目(AHKJ-02-2008) |
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| 摘 要： | 研究免疫磁珠法用于出口脱水蒜制品中沙门氏菌的检测，将免疫磁珠使用于400mL大体积溶液中，并使用高速离心代替磁棒进行分离。致病微生物捕获、分离实验结果表明免疫磁珠法具有特异性强、灵敏度高的特点，沙门氏菌污染量为1CFU/25g时，检出率为70%，污染量为10CFU/25g时，检出率达100%。
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| 关 键 词： | 脱水蒜制品 免疫磁珠 沙门氏菌ATCC 14028 |
| 收稿时间： | 2011-09-28 |
Detection of Salmonella in Dehydrated Garlic Products with Immunomagnetic Beads |
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| YU Xiao-feng,ZHANG Ping,ZONG Kai,YAN Yi,SUN Juan-juan,LI Yun-fei,ZHENG Hai-song,CHEN Xue-jiao. Detection of Salmonella in Dehydrated Garlic Products with Immunomagnetic Beads[J]. Food Science, 2012, 33(24): 257-259. DOI: 10.7506/spkx1002-6630-201224054 |
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| Authors: | YU Xiao-feng ZHANG Ping ZONG Kai YAN Yi SUN Juan-juan LI Yun-fei ZHENG Hai-song CHEN Xue-jiao |
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| Affiliation: | 1. Technological Center, Anhui Entry-Exit Inspection and Quarantine Bureau, Hefei 230022, China；2. Anhui Key Laboratory of Analysis and Detection Technology for Food Safety, Hefei 230022, China |
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| Abstract: | Immunomagnetic beads were used to detect Salmonella in dehydrated garlic products. A large volume of Salmonella suspension was added with immunomagnetic beads and high-speed centrifugation was used instead of magnetic bar for bacterial separation. The results of bacterial capture and separation showed that immunomagnetic beads were highly specific and sensitive. The detection rates were 70% and 100% for contamination levels of 1 CFU/25 g and 10 CFU/25 g, respectively. |
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| Keywords: | dehydrated garlic products immunemagnetic beads Salmonella ATCC 14028 |
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