As oxidation by Thiomonas arsenivorans in up-flow fixed-bed reactors coupled to As sequestration onto zero-valent iron-coated sand |
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Authors: | Junfeng Wan Stephane Simon Marie-Christine Dictor Christophe Dagot |
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Affiliation: | a GRESE EA 4330, Université de Limoges, 123 avenue Albert Thomas, F-87060 Limoges Cedex, France b BRGM, Environmental and Process Division, 3 avenue Claude Guillemin, BP 36009, F-45060 Orléans, France |
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Abstract: | The combined processes of biological AsIII oxidation and removal of AsIII and AsV by zero-valent iron were investigated with synthetic water containing high AsIII concentration (10 mg L−1). Two up-flow fixed-bed reactors (R1 and R2) were filled with 2 L of sieved sand (d = 3 ± 1 mm) while zero-valent iron powder (d = 76 μm; 1% (w/w) of sand) was mixed evenly with sand in R2. Thiomonas arsenivorans was inoculated in the two reactors. The pilot unit was studied for 33 days, with HRT of 4 and 1 h. The maximal AsIII oxidation rate was 8.36 mg h−1 L−1 in R1 and about 45% of total As was removed in R2 for an HRT of 1 h. A first order model fitted well with the AsIII concentration evolution at the different levels in R1. At the end of the pilot monitoring, batch tests were conducted with support collected at different levels in R1. They showed that bacterial AsIII oxidation rate was correlated with the axial length of reactor, which could be explained by biomass distribution in reactor or by bacterial activity. In opposition, AsIII oxidation rate was not stable in R2 due to the simultaneous bacterial AsIII oxidation and chemical removal by zero-valent iron and its oxidant products. However, a durable removal of total As was realized and zero-valent iron was not saturated by As over 33 days in R2. Furthermore, the influence of zero-valent iron and its oxidant corrosion products on the evolution of AsIII-oxidizing bacteria diversity was highlighted by the molecular fingerprinting method of PCR-DGGE using aoxB gene as a functional marker of aerobic AsIII oxidizers. |
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Keywords: | Thiomonas arsenivorans Biological AsIII oxidation Zero-valent iron aoxB gene Fixed-bed reactor |
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