Application of gas anti-solvent process to the recovery of andrographolide from Andrographis paniculatanees |
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| Authors: | Manop Charoenchaitrakool Wuttichai Trisilanun Penjit Srinopakhun |
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| Affiliation: | (1) Center for Pharmaceutical Biotechnology, Department of Chemistry and Biochemistry and CIRES, University of Colorado, 215 UCB, Boulder, Colorado 80309, USA;(2) Center for Pharmaceutical Biotechnology, Department of Pharmaceutical Sciences, University of Colorado Health Sciences Center, 4200 E. Ninth Avenue, P.O. Box C238, Denver, Colorado 80262, USA;(3) Aktiv-Dry LLC, 6060 Spine Road, Boulder, Colorado 80301, USA;(4) Biogen Idec, Inc., 5200 Research Place, San Diego, California 92122, USA; |
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| Abstract: | The gas anti-solvent (GAS) process was employed to extract andrographolide, which is the active ingredient found in Andrographis Paniculatanees, using carbon dioxide as an anti-solvent. The effects of temperature, flow rate and solvent type on the extraction recovery, particle size and morphology were investigated in this study. The experiments were conducted at the temperature ranging from 25–45 °C, carbon dioxide flow rate of 1–15 mL/min, and various types of organic solvents (methanol, ethanol, acetone and N,N-dimethylformamide). The extracted product was analyzed using high performance liquid chromatography (HPLC). The highest extraction yield was found to be 1.24 g andrographolide per 100 g of A. paniculata when using acetone as a solvent, carbon dioxide flow rate of 5 mL/min and the temperature of 35 °C. It was also found that no significant change in size or morphology of the precipitates was observed when changing temperature, carbon dioxide flow rate and solvents. |
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