| 温度和碳源对隐甲藻生长及DHA积累的影响 |
| |
| 引用本文： | 郭小婧,龚阳敏,梁焯,万霞,胡传炯,刘姣,江木兰.温度和碳源对隐甲藻生长及DHA积累的影响[J].中国油料,2013(5):597-603. |
| |
| 作者姓名： | 郭小婧 龚阳敏 梁焯 万霞 胡传炯 刘姣 江木兰 |
| |
| 作者单位： | [1]中国农业科学院油料作物研究所,湖北武汉430062 [2]农业部油料作物生物学与遗传育种重点实验室,湖北武汉430062 |
| |
| 基金项目： | 国家自然科学基金（31270346）;国家863计划（2011AA100904） |
| |
| 摘 要： | 研究了隐甲藻（Crypthecodinium cohnii ATCC 30772）以摇瓶发酵培养方式获得最大生物产量和最高二十二碳六烯酸（docosahexaenoic acid,DHA）产量的培养条件.结果表明,有利于隐甲藻生长和DHA积累的最佳培养条件为：培养温度为22℃,先以12g/L葡萄糖为碳源培养4d,后再以3g/L醋酸钠为碳源培养4d.由此获得的生物产量为234.85mg/（L·d）,DHA产量为17.45mg/（L.d）,DHA含量为74.36mg/g菌体.
|
| 关 键 词： | 隐甲藻 培养条件 生物量 DHA |
Effects of temperature and carbon source on growth and docosahexaenoic acid accumulation of Crypthecodinium cohnii |
| |
| Affiliation: | GUO Xiao-jing(Oil Crops Research Institute of Chinese Academy of Agriculture Sciences, Wuhan 430062, China) GONG Yang-min(Oil Crops Research Institute of Chinese Academy of Agriculture Sciences, Wuhan 430062, China) LIANG Zhuo(Oil Crops Research Institute of Chinese Academy of Agriculture Sciences, Wuhan 430062, China) WAN Xia(Oil Crops Research Institute of Chinese Academy of Agriculture Sciences, Wuhan 430062, China) HU Chuan-jiong(Oil Crops Research Institute of Chinese Academy of Agriculture Sciences, Wuhan 430062, China;Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China) LIU Jiao(Oil Crops Research Institute of Chinese Academy of Agriculture Sciences, Wuhan 430062, China) HANG Mu-lan(Oil Crops Research Institute of Chinese Academy of Agriculture Sciences, Wuhan 430062, China;Key Laboratory of Biology and Genetic Improvement of Oil Crops, Ministry of Agriculture, Wuhan 430062, China) |
| |
| Abstract: | Culture conditions were adapted to obtain larger biomass and higher docosahexaenoic acid （DHA） yield of Crypthecodinium cohnii under flask fermentation level.Results showed that the optimal culture should be at 22℃,in carbon-source culture with 12g/L glucose for 4d and then shifted to the same media but with 3g/L sodium acetate instead for 4d.Under this optimal condition,the C.cohnii biomass yield,DHA yield and DHA content reached 234.85mg/（L · d）,17.45mg/（L · d）,74.36mg/g dry weight respectively. |
| |
| Keywords: | Crypthecodinium cohnii Culture condition Biomass DHA |
| 本文献已被 维普 等数据库收录！ |
|
