| 田口设计优化曲霉产多糖培养基 |
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| 引用本文： | 赵玉萍,朱春. 田口设计优化曲霉产多糖培养基[J]. 食品科技, 2011, 0(7): 16-20 |
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| 作者姓名： | 赵玉萍 朱春 |
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| 作者单位： | 淮阴工学院生命科学与化学工程学院; |
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| 基金项目： | 淮安市农业科技计划项目(SN0868); 江苏省优秀青年骨干教师项目; 江苏省科技支撑计划项目(BE2010357) |
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| 摘 要： | 采用田口设计对多糖产量的培养基成分进行优化。先进行单因素试验,再利用Minitab软件设计正交试验,采用田口设计方法分析正交试验结果各因素水平的均值情况和信噪比情况。分析结果证明麸皮、硫酸镁和磷酸二氢钾对多糖产量影响极显著。软件预测培养基的最优组合为麸皮20g/L、酵母膏40g/L、MgSO4.7H2O0.25g/L和KH2PO41g/L,预测产量为8.86g/L,信噪比为20.1547dB。验证试验多糖产量为8.95g/L,信噪比为20.9213dB,优化方案达到了预期效果,具有较好的稳定性。
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| 关 键 词： | 多糖 Minitab软件 田口设计 培养基优化 |
Optimization of culture medium for polysaccharide production by Aspergillus using Taguchi design |
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| ZHAO Yu-ping,ZHU Chun. Optimization of culture medium for polysaccharide production by Aspergillus using Taguchi design[J]. Food Science and Technology, 2011, 0(7): 16-20 |
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| Authors: | ZHAO Yu-ping ZHU Chun |
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| Affiliation: | ZHAO Yu-ping,ZHU Chun(School of Life Science and Chemical Engineering,Huaiyin Institute of Technology,Huai'an 223003) |
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| Abstract: | In this paper,culture medium for polysaccharide production by Aspergillus was optimized using Taguchi design.First,single factor was tested,then orthogonal experiment was designed to carry out using Minitab software,and the results of the mean and SNR of level of each factor were analyzed using Taguchi design.The results proved that the effects of wheat bran,magnesium sulfate and potassium dihydrogen phosphate on the polysaccharide yield were significant.Software predicts the optimal culture medium consiste... |
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| Keywords: | polysaccharide Miuitab software Toguchi design medium optimization |
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