| 响应面法优化成曲蚕豆瓣子中黄曲霉毒素B1的提取 |
| |
| 引用本文： | 靳学敏,车振明,汪颖.响应面法优化成曲蚕豆瓣子中黄曲霉毒素B1的提取[J].食品科技,2011(1):200-204. |
| |
| 作者姓名： | 靳学敏 车振明 汪颖 |
| |
| 作者单位： | 西华大学生物工程学院; |
| |
| 基金项目： | 西华大学食品科学重点学科资助项目 |
| |
| 摘 要： | 郫县豆瓣中所用蚕豆是产自四川省和云南省的优质干蚕豆,经筛选、浸泡、接种、制曲等一系列加工后形成成曲蚕豆瓣子。采用酶联免疫吸附法(ELISA),根据Box-Behnken Design软件的设计原理,检测成曲蚕豆瓣子中黄曲霉毒素B1。探讨了甲醇浓度、料液比、超声波提取时间等因素对样品中黄曲霉毒素提取的影响,以确定检测生产过程中样品黄曲霉毒素含量的最优方案。以黄曲霉毒素含量为响应值,利用响应面分析法对提取参数进行优化。结果表明:甲醇浓度39.66%,超声波提取时间27.70min,超声波提取功率88.97%是检测成曲蚕豆瓣子中黄曲霉毒素含量的最优条件,以该优化条件检测成曲蚕豆豆瓣子中黄曲霉毒素其值达到最大,为1.47μg/kg。
|
| 关 键 词： | 酶联免疫吸附法 黄曲霉毒素 响应面法 |
Response surface methodology to optimize the extraction of aflatoxin B_1 from koji bean |
| |
| JIN Xue-min,CHE Zhen-ming,WANG Ying.Response surface methodology to optimize the extraction of aflatoxin B_1 from koji bean[J].Food Science and Technology,2011(1):200-204. |
| |
| Authors: | JIN Xue-min CHE Zhen-ming WANG Ying |
| |
| Affiliation: | JIN Xue-min,CHE Zhen-ming,WANG Ying (Biological Engineering Institute of XiHua University,Chengdu 610039) |
| |
| Abstract: | Bean used in Pixian soybean paste are produced in Sichuan province and Yunnan province,koji bean are produced by screening,dipping,vaccination and a series of process.In this paper,according to the box-behnken desigh software,we used the enzyme-linked immuneosorbent assay(ELISA) to test the valve of aflatoxin B1 in koji bean.Taking over the influence about methanol concentration,the amount of methanol solution and ultrasonic time on the extraction of aflatoxin in the samples to determine the best proposal.I... |
| |
| Keywords: | enzyme-linked immuneosorbent assay aflatoxin response surface method |
| 本文献已被 CNKI 等数据库收录！ |
|
