| 反胶束提取小麦胚芽蛋白的工艺研究 |
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| 引用本文： | 葛焕生,李书国,于新.反胶束提取小麦胚芽蛋白的工艺研究[J].食品科技,2011(1):133-137. |
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| 作者姓名： | 葛焕生 李书国 于新 |
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| 作者单位： | 仲恺农业工程学院轻工食品学院;河北科技大学生物科学与工程学院; |
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| 摘 要： | 以副产品小麦胚芽为原料,用反胶束法研究了小麦胚芽蛋白的提取条件,包括前萃和后萃。前萃由琥珀酸二(2-乙基己基)酯璜酸钠(AOT)-异辛烷-氯化钾缓冲溶液组成的反胶束体系从小麦胚芽中提取蛋白;后萃先回收异辛烷,少量KCl的缓冲溶液溶解剩余的固形物,最后用丙酮沉淀得小麦胚芽蛋白。通过单因素实验,考查了AOT浓度、萃取时间、加入小麦胚芽粉量、温度、KCl浓度、缓冲溶液pH、W/O对小麦胚芽蛋白前萃率的影响以及KCI浓度、缓冲液pH、缓冲液加入量对小麦胚芽蛋白后萃率的影响。
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| 关 键 词： | 反胶束 小麦胚芽蛋白 提取 |
Extraction of wheat germ protein by reverse micelles |
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| GE Huan-sheng,LI Shu-guo,YU Xin.Extraction of wheat germ protein by reverse micelles[J].Food Science and Technology,2011(1):133-137. |
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| Authors: | GE Huan-sheng LI Shu-guo YU Xin |
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| Affiliation: | GE Huan-sheng1,LI Shu-guo2,YU Xin1 (1.College of Light Industry and Food Science,Zhongkai University of Agriculture and Engineering,Guangzhou 510225,2.Biological Science and Engineering College,Hebei University of Science and Technology,Shijiazhuang 050018) |
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| Abstract: | In the article,the extraction of defatted wheat germ protein(DWGP) by reverse micelles was studied,it consists the forward extraction and the backward extraction.The reverse micellar system was formed by sulphosuccinic acid bis(2-ethylhexyl) ester sodium salt(AOT),isooctane and KCl buffer solution;After recovery of isooctane and dissolution of residual solid with a small amount of KCl buffer solution,the wheat germ protein was preciptated by acetone,while AOT remained in the acetonephase.Through the single-... |
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| Keywords: | reverse micelles wheat germ protein extraction |
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