Modulation of talA gene in pentose phosphate pathway for overproduction of poly-beta-hydroxybutyrate in transformant Escherichia coli harboring phbCAB operon |
| |
Authors: | Song Byoung-Geun Kim Tae-Kwon Jung Young-Mi Lee Yong-Hyun |
| |
Affiliation: | Department of Genetic Engineering, College of Natural Sciences, Kyungpook National University, Daegu, S Korea. |
| |
Abstract: | The talA gene encoding transaldolase, the key enzyme in the nonoxidative pentose phosphate pathway, was amplified in a transformant Escherichia coli harboring the phbCAB operon to shift the metabolic flux of the hexose mono-phosphate shunt to the odd-ball biosynthesis pathway for poly-beta-hydroxybutyrate overproduction. The PHB content in the transformant E. coli coharboring the phbCAB operon and talA gene increased from 28.2% to 52.3%, and the retarded cell growth was overcome. This increase seems to be mainly due to the concomitant supplies of the intermediates NADPH and acetyl-CoA, which are from the activated pentose phosphate pathway through the modulation of the talA gene and from the Embden-Meyerhof pathway. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|