Super‐Resolution Microscopy Unveils Dynamic Heterogeneities in Nanoparticle Protein Corona |
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Authors: | Natalia Feiner‐Gracia Michaela Beck Sílvia Pujals Sébastien Tosi Tamoghna Mandal Christian Buske Mika Linden Lorenzo Albertazzi |
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Affiliation: | 1. Institute for Bioengineering of Catalonia (IBEC), The Barcelona Institute of Science and Technology, Barcelona, Spain;2. Inorganic Chemistry II, Ulm University, Ulm, Germany;3. Advanced Digital Microscopy Core Facility (ADMCF), Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology, Barcelona, Spain;4. Institute of Experimental Cancer Research, University Hospital Ulm, Ulm, Germany |
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Abstract: | The adsorption of serum proteins, leading to the formation of a biomolecular corona, is a key determinant of the biological identity of nanoparticles in vivo. Therefore, gaining knowledge on the formation, composition, and temporal evolution of the corona is of utmost importance for the development of nanoparticle‐based therapies. Here, it is shown that the use of super‐resolution optical microscopy enables the imaging of the protein corona on mesoporous silica nanoparticles with single protein sensitivity. Particle‐by‐particle quantification reveals a significant heterogeneity in protein absorption under native conditions. Moreover, the diversity of the corona evolves over time depending on the surface chemistry and degradability of the particles. This paper investigates the consequences of protein adsorption for specific cell targeting by antibody‐functionalized nanoparticles providing a detailed understanding of corona‐activity relations. The methodology is widely applicable to a variety of nanostructures and complements the existing ensemble approaches for protein corona study. |
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Keywords: | heterogeneity mesoporous silica nanoparticles protein corona super‐resolution imaging targeting |
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