| 电化学沉积二氧化锆制备DNA电化学传感器 |
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| 引用本文： | 刘百祥,邵志清. 电化学沉积二氧化锆制备DNA电化学传感器[J]. 传感技术学报, 2011, 24(12): 1672-1675 |
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| 作者姓名： | 刘百祥 邵志清 |
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| 作者单位： | 华东理工大学物理系;华东理工大学计算机科学与工程系 |
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| 摘 要： | 设计了一种简单、方便的电化学DNA生物传感器的制作方法,首先在硼掺杂的金刚石电极(BDD)表面电化学沉积一层二氧化锆薄膜,利用二氧化锆的分子结构特点,将探针DNA即5’末端修饰磷酸基团的寡核苷酸短链(ssDNA)连接到二氧化锆薄膜上.以亚甲基蓝(MB)为氧化还原的电子媒介体,应用循环伏安法和差分脉冲法测试了该电极的性能...
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| 关 键 词： | 硼掺杂的金刚石 二氧化锆 生物传感器 亚甲基蓝 |
Zirconia Modified Electrochemical DNA Biosensor |
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| LIU Baixiang,SHAO Zhiqing. Zirconia Modified Electrochemical DNA Biosensor[J]. Journal of Transduction Technology, 2011, 24(12): 1672-1675 |
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| Authors: | LIU Baixiang SHAO Zhiqing |
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| Affiliation: | 1.Department of Physics,East China University of Science and Technology,Shanghai 200237,China2.Department of Computer Science and Engineering,East China University of Science and Technology,Shanghai 200237,China |
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| Abstract: | An electrochemical DNA biosensor based on the boron-doped diamond(BDD)electrode modified with zirconia(ZrO2)was developed,the zirconia thin porous film was fabricated by cyclic voltammatic method in an aqueous electrolyte of ZrOCl2 and KCl at room temperature,DNA probes were attached onto the ZrO2/BDD electrode due to the strong binding of the phosphate group of the DNA with the Zirconia film and the excellent biocompatibility of the BDD.DNA immobilization and hybridization were characterized by cyclic voltammetry and differential pulse voltammetry,using methylene blue(MB)as indicator.After the hybridization of DNA probe with the complementary DNA,the peak current of MB decreased obviously.The response current was linearly related to the concentration of the target oligonucleotide sequence in the range 2.15×10-10 mol/L to 2.15×10-7 mol/L. |
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| Keywords: | boron-doped diamond zirconia biosensor methylene blue |
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